2
votes
1answer
393 views

How to prepare sample for SDS PAGE?

I usually take my protein sample 0.8ml and sample buffer(2X) 0.2ml for my sample preparation in SDS PAGE. Am I using correct proportion? my protein sample concentration is 4.1mg/ml. What is the ...
2
votes
0answers
27 views

Which agricultural crops attract rodents?

Are there any data on rodent populations per acre of different types of agricultural crops?
0
votes
2answers
36 views

Resource for finding what proteins bind?

I have two lists of proteins (including phosphorylated forms). I want to determine which members of each list act on which members of the other list. What online resources exist for finding out what a ...
2
votes
1answer
134 views

Why do chameleons move back and forth?

I was always curious, why do chameleons have this strange gait?
19
votes
1answer
3k views

Why do we have five fingers?

Humans (and other humanoid mammals) have five fingers in each hand. Curious to know, why five? Wouldn't more fingers be more useful? Is there any evidence that it used to be different and natural ...
0
votes
1answer
197 views

Apoptosis vs necroptosis

I understand that apoptosis and necroptosis share the same upper part of the pathway, but I cannot seem to distinguish when is each one activated? From my readings, it seems that when procaspases 8 or ...
0
votes
1answer
45 views

Clarification on BMI formula

In the BMI formula= (Weight) X 703) / (Height^2) , what does 703 represent? What is a reliable source that I can cite for this answer? weight= lbs height= inches
1
vote
2answers
1k views

Book Recommendations: GRE Subject Test In Biochemistry, Cell And Molecular Biology

There are probably a lot of really good answers that may vary significantly in terms of content. I'm looking for a set of books that I can read in preparation for the GRE Subject Test In ...
1
vote
1answer
21 views

What is the most common way of administering loop diuretics and what is their time course of action?

I am reviewing some material on loop diuretics, and I am curious to know how these drugs are administered. Also, I am interested in knowing their time course of action once they are administered into ...
2
votes
1answer
47 views

What are the features on a microscope one needs in order to do lab work?

By lab work I mean urinalysis, blood work(live as well), fecals, cytologies, histologies and all other. I have read(partly) a book(from 2002) on lab diagnostics and the author did not mention ...
0
votes
1answer
54 views

Is “Cisternal Twins” an actual classification of twin types and if so, describe it

I always assumed my brother and I were identical twins. Now my mother, in her eighties, says we are cisternal twins, but I cannot find any references on the matter. Is there such a thing? If so, what ...
2
votes
0answers
130 views

Pink E.coli cell pellet. Reasons? [closed]

I've harvested IPTG induced E.coli BL21DE3 cells' suspension culture by spinning at 5000 rpm/15 min/4 degrees C. The pellet after spin looked pale pink colored. What could be the reasons?
7
votes
1answer
84 views

B cell receptor editing

If a B cell contains two mu chains and two lambda chains and is self reactive can it go back and rearrange its kappa light chains? I'm not sure if it can only try to rearrange its lambda light chains ...
6
votes
1answer
249 views

Find protein homologs with BLASTp

I'm trying to find homologs of a set of proteins using BLASTp. I'm working with custom databases. I'm using evalue of 0.00001 as threshold. I would like to filter queries having hits with >90% ...
4
votes
1answer
32 views

Is genetic drift necessarily deleterious?

I read that genetic drift is random variation in the relative allele frequencies of a population. This question however seems to pin genetic drift down to increasing deleterious allele ...
3
votes
1answer
34 views

Gene Sequencing and Plasmapper

Is there anything similar to this in Java (especially the circular map sequencing along with hover effect)? For information I would like to convey that I am using Plasmapper and BioJava for achieving ...
1
vote
0answers
17 views

Recombination frequency as a function of genomic location in E coli?

Are there any databases or papers that give recombination frequency as a function of position in the E coli genome?
1
vote
0answers
20 views

Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
4
votes
1answer
105 views

How small does a nanobot have to be to “swim through the brain” and access any neuron it wants to?

I read on this question What is in the space between neurons in a brain? that there is actually not much empty space in a brain. But my question is slightly different. Is there a visual demonstration ...
1
vote
1answer
24 views

Centriole genes Knock-out Experiment in Common experimental animals?

Anyone know of any experiments that have knocked out the genes for producing centrioles in a worm, mouse, fish, fly or whatever animal? Are the genes for centrioles even identified? It has been shown ...
2
votes
1answer
61 views
3
votes
2answers
129 views

DNA polymerase I exonuclease activity

Does DNA polymerase I in bacteria use forward or reverse exonuclease activity to remove RNA primers? One of my books says it uses 5' to 3', but another says it uses 3' to 5' exonuclease activity. ...
1
vote
1answer
30 views

Where do I purchase a fly with the collagen tagged?

Where can I purchase a fly with tagged collagen? My senior undergraduate research paper is observing the collagen in larvae with normal diet compared to larvae with an experimental diet. I need ...
1
vote
1answer
46 views

Is the nuclear envelope present in G1 of interphase?

Is the nuclear envelope present in G1 of interphase of eukaryotic cell? If so how does a method like Calcium mediated transfection get DNA past the nuclear envelope?
4
votes
2answers
66 views

Do mosquito repellent sprays work on all insects?

I would like to know the mode of action of mosquito repellent aerosols. Are these specific to mosquito or are other insects affected too?
0
votes
1answer
41 views

Does vitamin D form crystals in the blood with calcium and magnesium?

I read on a vitamin-D product (2000NE) that I should not use calcium and magnesium with it, because it can result crystal formation in the blood. I googled, but find anything about this. Is it true? ...
5
votes
2answers
850 views

Why is the DNA codon table “equal” to the RNA codon table

Before anything else please pay attention of the double quotes on the "equal" in the title - I know they are not equal, but you will understand in a bit. If I look at the DNA codon table here or in ...
1
vote
1answer
107 views

How to separate peptide on SDS PAGE?

I want to separate 1600 Da peptide on SDS PAGE, on 20% GEL I didn't get any band, How can I separate on PAGE? Can I use 25% SDS PAGE?
1
vote
1answer
579 views

How to translate a blood type used in Eastern Europe?

What are the I-IV blood type descriptions shown below (commonly used in Eastern Europe), and how do you translate them into the ABO-system?
0
votes
1answer
46 views

Do hairs in the ear respond to things like shampoo?

Shampoo and conditioning products make claims to be the end all be all in hair care. I doubt that they are so advanced "growth formula/anti-breakage/oily treatment". Have any of the claims been shown ...
4
votes
1answer
55 views

Comparative leg sizes

When I was a child, my father showed me the classic essay "On Being the Right Size", J. B. S. Haldane. It talks (among other things) about how large animals need stockier legs to support their ...
2
votes
2answers
89 views

How does the cell regulate different metabolic pathways?

I heard somewhere that cells use different nucleosides bound to triphosphates e.g. ATP, GTP, CTP and other modified compounds: NADH, NADPH to distinguish between different metabolic pathways and so ...
2
votes
2answers
78 views

Does the cellular response to every receptor work the same way?

I heard somewhere that activating any receptor results in the same intracellular response (signaling) which involves NF-κB. If that is true, I hardly understand how the cells distinguish between ...
0
votes
0answers
12 views

Ambidexterity Advantages and Disadvantages? [duplicate]

I was looking into training myself into being ambidextrous, what are the neurological and physical advantages and disadvantages of learning (not being born with) ambidexterity [if there is a ...
2
votes
1answer
35 views

Confusion over role of current/potential in Nervous system?

I am taking a course on the nervous system and I am do not have any physics background. Therefore, I would like to know, what are we precisely talking about when we talk about current in the nervous ...
0
votes
2answers
103 views

Can Anyone Identify This Bug

I'm looking for someone to identify this bug. Thanks!
1
vote
1answer
241 views

How can I get brighter DNA bands?

I amplified my DNA with real time PCR. The amplification curve shows good amplification but not a single band is observed in my gel after gel electrophoresis. What would be the possible reasons? I ...
3
votes
1answer
56 views

Confusion regarding niches in evolution theory

This is the definition of a niche from Crash Course: An area of the environment that requires a special set of skills or traits to extract food and reproduce. Obviously, this version of the ...
5
votes
6answers
1k views

Can we harvest energy from plants?

This is a cross-cutting question but I think its core is about biology. Our society's need for energy is dramatically growing and we are messing up with our environment to answer them. Maybe another ...
7
votes
2answers
720 views

Does muscle get bigger by increase in size of individual cells or increase in number?

Somewhere in the back of my mind, I have the claim that a muscle never increases its amount of cells but, if the muscle gets bigger, it's simply because individual cells get bigger. The book Anatomy ...
1
vote
1answer
367 views

Do ants feel pain?

I have watched video on youtube where guy pours molten aluminium into fire ant colony to make casing. In the comments below there's huge discussion on is that a right thing to do. I am on the side ...
2
votes
1answer
58 views

What kind of animal can do this?

When I was moving to another house, I found this plastic bag on the top of the cabinet of my old house. It looks like someone cut it all with a scissor, but no one have access to my bedroom, and it ...
5
votes
1answer
88 views

Weird gelatinous substance in highland bog

I recently went walking in the highlands in Snowdonia, Wales and we came across this: Can anyone explain what it is? My guess was some kind of fungus but I'm not positive. The ground was very water ...
4
votes
1answer
74 views

Criteria for compound action potential thresholds

As opposed to action potential thresholds (which are binary yes/no events), electrophysiological thresholds of compound action potentials are arbitrary. Mostly a certain noise level is picked and when ...
4
votes
1answer
110 views

Will genetically modified food affect our health?

It's a popular public sentiment that - GM foods like tomatoes (flavr savr) will affect our health.. Is there any logical scientific explanation behind this?
0
votes
1answer
60 views

How do fish communicate?

Fish, as far as I know ( of course there may be exceptions) , don't have any vocal organ...but they are proven to be social...so how do they manage to communicate with each other...on land, organisms ...
3
votes
1answer
81 views

Which is better against genetic mutations? Asexual or Sexual Reproduction?

In terms of genetics, which type of reproduction is better against adverse mutations? I understand a bit of Muller's Ratchet—in which asexual organisms accumulate a number of deleterious mutations in ...
1
vote
1answer
140 views

Can one use a hemocytometer on a compound upright microscope?

I'm posting this as a follow-up on What to look for when buying a light microscope?. The answerer states that you would need to use a an inverted microscope to count cell in the hemocytometer(counting ...
4
votes
2answers
2k views

Importance of Double Helix DNA Structure

Gene expression involves transcribing only one strand of DNA molecule. So i was wondering what are some biological advantages of the double stranded DNA?
5
votes
3answers
107 views

Why don't fish groom each other?

Fish are often plagued by external parasites, which are presumably difficult for them to remove. There are well known cases where large fish come to coral reefs and allow smaller fish to pick off ...

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