3
votes
1answer
96 views

How do different tissue culture matrices affect background in fluorescent microscopy?

In response to my previous question, I've been reading up a little bit on poly-D-lysine, Collagen I, Collagen IV, laminin, and other tissue culture coatings that promote cell adhesion. I've always ...
1
vote
0answers
40 views

What parts of the brain are involved in regulating blood pressure in mammals?

I'm reading this Wikipedia article on blood pressure and its regulation, and it mentions that medulla is involved. however, I vaguely remember reading another article on Wikipedia and it mentioned ...
4
votes
1answer
77 views

Measuring sugar content of a tree

I would like to grow mushrooms on oak trees I can harvest from my property. I've read that the best results will be when the sap is rising but the tree hasn't budded yet. The mycelium feeds on the ...
4
votes
2answers
543 views

How to convince suspension cells to adhere more tightly?

I'm developing a cell-based assay in 96-well plates that requires adherent cells, as they need to be washed at least twice during the protocol. I'm using in-house strains of HT1080 cells (some ...
3
votes
0answers
48 views

Excretion of monovalent and divalent ions in sharks

I have heard that sharks excrete $\ce{Na+}$ and $\ce{Cl-}$ by their gill surfaces but divalent ions like magnesium are excreted through feces. What could be the reason behind this?
1
vote
1answer
230 views

How do changes in temperature affect body weight in humans?

What would be effect on weight of individuals if we bring a fully adapted (to cold climate ) human individual and a fully adapted to very hot climate individual to a moderate climate region?
0
votes
1answer
54 views

Why is epinephrine not part of the somatic nervous system?

I learned that the adrenal medulla releases epinephrine, which is a vasodilator of the vessels that lead to the skeletal muscle. I also learned that somatic nervous system only uses Achetycholine as ...
2
votes
2answers
81 views

Evolution after the development of sexual reproduction

My understanding of evolution is that genetic mutation occurs in individual members of a species, and they become a new species. Isn't a definition of species a group of genetically similar organisms ...
3
votes
1answer
94 views

Superposing DNA

I have a series of protein models with DNA docked. I now want to superpose the DNA on a reference DNA molecule and extract the translational distance applied and the rotation angle used. I can ...
2
votes
1answer
98 views

Does precipitation influence the UV spec reading?

I have been checking my sample for Hydroxyl radical scavenging activity. I have dissolved Hydrogen peroxide in Phosphate buffer. My sample is clear and reagent is also clear but when I have mixed my ...
6
votes
2answers
128 views

Can any species be bred selectively/engineered to become as diverse looking as dogs?

I've done some research and it appears that dogs are the most diverse looking single species of mammals. The questions that interest me is - are dogs special in respect to genes/gene activation ...
4
votes
1answer
80 views

What determined the evolution of different faces in humans?

The most distinctive characteristic of a human is it's face - it is unique among each individual (with the exception of identical twins). It is uncertain to me if whether we best identify other humans ...
3
votes
0answers
71 views

Food Intake versus ability to flee among birds, particularly the hummingbird?

Logically speaking, if a hummingbird drinks too much nectar, it will be temporarily overweight and less able or unable to fly to escape danger. However if the same hummingbird doesn't drink enough ...
2
votes
1answer
195 views

What is this insect?

These photographs were taken today in Marseille, in the Mediterranean coast of France. My French friends inform me that these beasties are called cousins because they are "cousins" of mosquitoes ...
7
votes
1answer
84 views

How do animals perform detailed active camouflage?

We often see videos of octopuses and chameleons changing their color to suit their surroundings. If it was a simple color change from white to red, it'd be understandable, but some of these animals ...
3
votes
1answer
77 views

Can you cure allergies?

A common solution is allergy shots, which helps you adapt to the allergy. But are there ways to tell the body that this allergy is safe and there is no need to amount a response to it? So that there ...
-2
votes
1answer
269 views

Are we evolving to age slower, or to live longer aging at the same rate?

I wonder why most of us find senior people lovely and charming. Is it because to love grandpa and granny is vital to our survival as a species, in the same way as we love babies? If so, it would mean ...
3
votes
1answer
66 views

Would non-human carnivores be able to survive on farmed foods?

Certain creatures that are considered carnivores are non-obligate carnivores and are technically omnivores (such as wolves/dogs). How different is the potential digestive ability of these species ...
2
votes
1answer
430 views

Optogenetics - How do microbial opsins work?

I'm just introduced to the optogenetics method and am having some trouble grasping the genetics (of the optogenteics) part of things. So we have Retinal and Opsin that form Rhodopsin molecule that ...
11
votes
1answer
1k views

Why does oxygen bind to hemoglobin in an specific angle?

There is an specific angle of the bond between oxygen and hemoglobin in lungs, which I don't know, but what interests me more is the question, why is it specific?
1
vote
0answers
46 views

Removing bacterial contaminants from resin

We have a few Strep-tactin columns that had some growth in them and we would like to regenerate the columns back since the resin is quite expensive. Basic goal: remove the brown stuff. So far, I've ...
0
votes
1answer
45 views

What is a good review article source for Physiology?

I am looking review articles for Physiology. For instance, Physiological explanation of steps of measurement of the metabolism by Douglas-Haldane. There is no section for Physiology or metabolism in ...
2
votes
1answer
183 views

How do fully dioecious plants determine sex?

I know that a minority of plants are fully dioecious, with adult plants producing either only male or only female flowers. When is this sexual differentiation determined? Is the seed of such a plant ...
2
votes
1answer
63 views

Identify this waterfowl species?

I've been unable to find anything on the Google resembling this bird. I took the photo in Virginia Beach, VA around 2010, October, I think... It appears to be between winter and summer plumage but I'm ...
5
votes
1answer
134 views

Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?

I'm currently working on a synthetic biology project which involves working with lots of different parts. I would ultimately like to integrate these genes by transforming a single plasmid. I've heard ...
5
votes
0answers
166 views

Do non-human animals suffer from insomnia?

And how do we know whether they do or not? § If sleep is a physiological need, it seems weird that we become unable to fulfill on demand even in the absence of any obvious physical issues. We would ...
2
votes
1answer
135 views

Can antibodies be formed against white blood cells after blood transfusion?

If antibodies are produced against other blood groups' red blood cells, why can't antibodies form against white blood cells, of any blood group? (even the same one, as MHC will be different in almost ...
3
votes
1answer
107 views

What is the difference between different brain regions

The brain is separated into different regions, and different regions perform different tasks. Well, what are the differences between these regions on the cellular/systemic level. The brain is made up ...
5
votes
3answers
248 views

Can extinct animals be cloned?

Scientists have found mammoth blood, and are planning to clone a mammoth. How does one go from having its blood to a full blown living mammoth? Is it possible? Why does it matter if the blood is ...
2
votes
0answers
57 views

Would my hands sweat this much if I wasn't wearing gloves?

I've wondered about this for a long time, and hopefully someone here has a reasonable answer. I'm a cell and molecular biologist, and much of my time at the bench is spent wearing (usually nitrile) ...
2
votes
1answer
208 views

Ammonia smell after physical exercise? What is the explanation? [duplicate]

After exercising vigorously one can sometimes smell ammonia and it feels like it's coming from within the nose actually. There is no indication that others can smell it when I do (i.e. seems to be ...
3
votes
1answer
149 views

What is the best way to clean plastic flasks that have been used for cell cultures - is virkon a good idea?

When you use cultures e.g. insect cells, which are infected with virus one way to clean the (plastics) shake flasks is with virkon. Which is the most effective way to clean your flasks in order to ...
1
vote
0answers
115 views

Ligase Chain Reaction: Determining the annealing temperature for gene synthesis

Using the tool Gene2Oligo I have a set of DNA oligonucleotides to synthesize a gene using ligase chain reaction (LCR). The average melting temperature is 72 degrees Celsius. I am going to use a the ...
3
votes
1answer
334 views

Meat and mushroom allergies, why are they so rare?

There are a lot of food allergies, but for some reason not to meat (mammalian meat or poultry) or mushrooms. I've googled a bit, and now I know that one can actually have a meat or mushroom allergy, ...
1
vote
2answers
92 views

Inverse of dopamine reuptake inhibitor?

The wikipedia article on the dopamine transporter gives examples of some drugs whose effects are mediated by the inhibition of the dopamine transporter, such as cocaine and amphetamines. Are there ...
1
vote
2answers
341 views

What kind of tree could this be?

This may be a strange question, but does anyone know what kind of tree this could be? I know, it's just a comic, but these trees, at “walking distance” within that comic are easily identifiable as ...
2
votes
3answers
108 views

Blood pressure during exercise

What happens to blood pressure when we exercise? Does it increase, decrease, or remain the same? If it does increase, why are patients with high blood pressure recommended to exercise?
5
votes
1answer
193 views

Skeletal muscles in clam

Although skeletal muscle fatigues fairly rapidly, clams have a protein called paramyosin that allows them to sustain contractions for up to a month. What might be the role of paramyosin at the ...
2
votes
1answer
100 views

What kind of mushroom(?) is this?

I found groups of these growing in the Lassen National Forest (Susanville, CA, USA). It looks like some sort of mushroom. I have Googled extensively, but can't seem to find a match.
2
votes
0answers
44 views

Why do conker flowers grow upwards?

Most trees I know that have their flowers in groups hang these groups down. I noticed, however, that conker flowers grow upwards! I guess that growing against gravity requires special effort, so ...
1
vote
0answers
38 views

To what extent is immature eggs in insects (beetles) a good approximation for maximum fecundity?

To what extent is the number of immature eggs from dissected, newly emerged individuals a reasonable approximation for maximum/potential fecundity in insects (more specifically beetles)? I know that ...
1
vote
0answers
33 views

Are there precedents for considering that the coral reef biome has higher biodiversity than rain forests?

I've found conflicting references on which biomes are the most biodiverse ones. Also, which are the measures of biodiversity? Average number of different species per volume?
5
votes
1answer
364 views

What happens to an ant colony when the queen dies?

Does the colony collapse? Do workers keep following the last orders? Or can future queens replace the dead one? I'm guessing this might also depend on each specific sub-species.
1
vote
1answer
84 views

What are the characteristics of a promising drug target?

When researchers are looking to start developing a drug, what characteristics do they look for in the potential proteins (assuming they already have good quality structural models)?
1
vote
0answers
54 views

Are there any examples where 'magic bullet' drugs have worked?

Magic bullets are drugs that can be administered on a micro local scale near the tumour by exploiting the different surface antigens that cancers expose. The drug attaches via an mAb (attached to the ...
1
vote
1answer
48 views

What does “tumour budding” mean?

tumour budding, lymphocytic infiltration and resection margins are established factors that influence the outcome of colorectal cancer (1) In this context what does "tumour budding" mean? Reference ...
3
votes
1answer
921 views

How do liver and lung metastases cause death?

Metastasis to the liver and lungs are the main causes of death in colorectal cancer.(1) I understand that colorectal cancer may have metastases. But how do these metastases cause death? Reference ...
0
votes
0answers
31 views

Ovalbumin Detection

Is there a practical way to detect whether or not a baked good has egg in it? I'm thinking of something that can be brushed on to evolve a color change. Any help is appreciated.
5
votes
1answer
197 views

Would a spinal injury decrease human height?

A woman I know has been losing height at a rate of few cm a year following a severe spinal injury. How is this possible? What kind of injury could cause such progressive loss of height? Could this be ...
0
votes
2answers
111 views

What beetle is this?

I've found it in my kitchen. It seems to be that he flew through the window from the street. The country is Russia.

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