0
votes
1answer
60 views

Interpretation of faint band for Linear Array HPV Genotyping Test

After having used the test for research it would appear as if it is very specific. What is one to make of bands that are there but faint? Or what if there are two bands and one is big and bold and the ...
2
votes
3answers
482 views

Amplification technique for proteins similar to PCR for DNA?

I know PCR can be used to amplify a tiny sample of DNA in order to perform experiments. Is there a similar technique to use on a protein sample? More specifically, I'm not interested in "cutting" up ...
6
votes
1answer
80 views

What's the name of this bug from Toronto, Canada?

It's about 3 millimetres in length. Found on September 10, 2015.
3
votes
1answer
116 views

What is this plant?

It was found in Eastern Pennsylvania (in the United States) in a deeply wooded area.
0
votes
0answers
78 views

Root etiology of non-pituitary low T4 and low TSH

Please note: I'd like to preface this question by stating that this is neither homework nor me seeking medical advice. I am simply trying to understand the biological, physiological root etiology ...
3
votes
2answers
236 views

How does Cro protein expressed by lambda phage kill its host?

I read that the DNA segment of lambda phage integrated in host DNA could switch between lysogenic state where cI represses the expression of Cro and lytic state where Cro expression takes over and ...
2
votes
1answer
42 views

Are there emotions that motivate pattern completion? If so, where in the brain are they located?

I have been studying musical "tension." Musical tension essentially refers to the "expectations" one builds up in a melody that are then "released" when a stimulus matching the expectation occurs. For ...
2
votes
1answer
23 views

What is the use of crystallized Photosystems from cyanobacteria?

I read a lot of articles about techniques for the crystallization of Photosystem I and II from cyanobacteria. My question is what is the reason for that and what can you do with Photosystem crystals?
0
votes
2answers
127 views

What do Illumina HiSeq/MiSeq paired end reads look like?

My understanding is that paired end reads from the Illumina HiSeq/MiSeq platforms looks something like this: R1: AAAAAACCCCCC R2: GGGGGGTTTTTT Where the ...
0
votes
0answers
21 views

What can cause diploid offspring being produced by a neotetraploid seed parent?

Although in Rhododendron neotetraploid (seed parent) X diploid (pollen parent) produces a high percentage of triploids, flow cytometry of resulting seedlings from our hand crosses indicates both ...
2
votes
1answer
34 views

gene duplicated on genome but is different

I've been looking at a miRNA cluster on a genome and I found it three times. The first two are right next to each other and look exactly the same, the third sequences is on a different scaffold of the ...
1
vote
1answer
80 views

term for species that produces males only to mate with mother or sisters

In reading about unique reproductive strategies I've run into two closely related and comparatively unique mechanics. Both involved a female who was born 'pregnant' with at least a male, but the male ...
0
votes
4answers
233 views

How to convert FASTQ file format into GTF file format?

I have a plenty of FASTQ files (FASTQ is a standard for storing the output of high-throughput sequencing instruments such as the Illumina Genome Analyzer) and need to convert them to GTF format (gtf - ...
3
votes
1answer
146 views

What is the last heavy atom of an amino acid?

I'm a electrical engineer and I'm learning about bioinformatics. I was reading this paper that uses the last heavy atom to search for active sites of a protein but what would be a heavy atom? Is it ...
2
votes
0answers
49 views

Variance in reproductive success and effective population size

Background The effective population size $Ne$ is the size of the Wright-Fisher population that experience the same amount of drift than the population under consideration. The higher the variance in ...
2
votes
2answers
65 views

Troubleshooting PCR Steps [closed]

I am troubleshooting a PCR reaction that gives me no product, and there are a lot of different approaches that I can find in books and just in google searching (things like running a gradient, ...
1
vote
1answer
78 views

What is the evolutionary tradeoff against primates producing multiple offsprings (litter)?

If we evolved to have multiples all the time we would more likely have extended families living together in 1 house. This would be better than the spouses by themselves for multiple reasons including ...
7
votes
2answers
433 views

Why Egg shell is not called a cell wall?

Egg is a single cell and has a outer hard covering outside inside which there is a cell membrane. Then why isn't the egg shell a cell wall? Is it because no exchange of materials take place through ...
5
votes
1answer
37 views

Colorado potato beetle diet

The Colorado potato beetle is best known for being a potato pest. However, I've just learned that it originates from Northern America and the potato itself comes from South America. So the question ...
6
votes
1answer
138 views

What are those huge plants / fungi on trees called?

I've seen them in Scotland in August 2015: The plants / fungi were quite high (over 2m) on the tree. They are almost circular if you view them from the top, I guess (except for the part where they ...
1
vote
1answer
48 views

What is the name of the plant with the down-falling light purple leaves?

I've seen this flower in Scotland in August 2015: What is the name of that flower? To help people google it: The plant has light purple leaves with some white spots. On those white spots are dark ...
4
votes
1answer
74 views

What plant is it?

Do you know what plant is it? I found it in France
0
votes
0answers
20 views

Model for calculating muscular activity from external measures

A physiologist wants to measure the intensity of the activity of a muscle or muscle group over a certain period of time. But the physiologist cannot measure what happens inside the the muscle itself ...
5
votes
1answer
47 views

Length of dispersal flight of European spruce bark beetle?

I'm interested in length of dispersal flight of Ips typographus. I can barely find some information about dispersal distances, height of dispersal flight, but not at all information about how many ...
1
vote
0answers
26 views

How quickly can lasers of various wavelengths and powers impair human vision or cause pain in the eyes?

There is a discussion on HackerNews about how self-driving cars might be susceptible to confusion attacks using a cheap laser pointer and a RaspberryPi. Some people argue that human drivers, too, are ...
0
votes
1answer
92 views

difference in genetic instuctions between male and female [duplicate]

I'm a computer science graduate so please bear with me the following computer program : mov ecx, -1 INC ecx consist of 2 instructions (mov,inc) each working on specific data , can genetics ...
1
vote
0answers
104 views

How to interpret this cuffcompare result? Sensitivity over 100?

I run a cuffcompare: cuffcompare -r transcripts.gtf TransStandard_1.0.gtf I got the following result: ...
0
votes
0answers
30 views

Consumption of different type of energy when one is inactive

I'm wonder if there is any study done on this and what are the results? how much of each type of energy one's body(healthy normal person) uses when inactive(seating down) I know there are alot of ...
1
vote
0answers
22 views

Will bacterial promoter without either -10 or -35 sequence allow no transcription?

I have a strong constitutive promoter on a plasmid inside of E.Coli, which expresses a gene. Having either -10 or -35 sequence modified to random sequence, will it ensure zero transcription from that ...
2
votes
2answers
90 views

It's right to say coding sequence is part of exon sequence?

Some basic ambiguities making me confused. I downloaded 5'UTR, CDS, 3'UTR, and exon sequences, separately from Biomart for a gene P4HA2 (Homo sapiens) and found some simple sequence repeat (SSR) on ...
1
vote
1answer
25 views

Cell-type specific methylation profiles: original reference finding?

This is a general question, and a specific question. I'm looking for the original reference that first talked about how DNA methylation profiles are cell-type specific. This is specific, because I ...
0
votes
1answer
60 views

IBD-value in pedigree with inbreeding

I have a question about IBD. Please see the figure below. How many pairs of alleles are IBD for X and Y, or what is the IBD value for X and Y. Is it four (4)? I have seen it to be maximally 2, but ...
3
votes
1answer
76 views

How C. Elegans is used for siliencing genes

The experiment that is using C. Elegans to silence the Genes. I have a question about Why and how C. Elegans can use the DNA plasmid that is generated with the gene of interest in the bacteria by ...
0
votes
0answers
14 views

What is the range of “reasonable” counts of a bisulfite sequencing?

I analyzed the bisulfite sequencing data of a mouse sample (1 lane, I think) and it got enormous methylated/unmethylated counts on some chromosomes, e.g. chromosome 2. I thought it was a very a ...
0
votes
0answers
11 views

how is humidity related to amount of water we should drink [duplicate]

Is there any kind of relation between humidity and the amount of water we should drink. I know there is a relation between the temperature and the amount of water needed by the body. Do we need to ...
28
votes
1answer
7k views

Why does a coconut have exactly three holes

A theory says: As coconut is a sibling of palm, somehow long time ago, three palms were in a same husk. Based on evolution theory, it's how the coconut was born in the world with three holes. ...
2
votes
1answer
108 views

Dwell time equations for ATP-sythase?

I have read that every 120 degree rotation of the F1 complex of ATP-synthase can be split into a 30 degree rotation and a 90 degree rotation. In between these two are dwell times, the one before the ...
3
votes
1answer
121 views

What is a holocentric chromosome?

I was doing this question that asked: "How many centromeres does a typical chromosome have?" I thought one and the answer was:"One, except for holocentric chromosomes." So then what are ...
0
votes
1answer
60 views

Why are siblings unidentical? (Which chromosome of the pair do gametes have?) [closed]

I know that a normal human cell has 23 pairs of chromosomes (total: 46). On the other hand, a gamete has only 23 chromosomes. Which chromosome does the gamete choose out of each pair? And if "One of ...
3
votes
2answers
69 views

Dragonfly Identification

I have been clearing brush by a lake in northwest Missouri and seen a lot of dragonflies. It's hard to get decent pictures because my phone camera sucks and they fly away before I get close. But here ...
0
votes
0answers
81 views

Overcoming Inbreeding Depression

When inbreeding depression occurs, a genetically unrelated individual is mated with the animal to introduce genetic variability and remove homozygosity. In this case either outbreeding or ...
1
vote
1answer
150 views

Why do the deoxyribonucleotides use T and ribonucleotides use U? [duplicate]

We all know deoxyribonucleotides form DNA while ribonucleotides form RNA, DNA is double stranded while RNA is single stranded, and RNA can transcribe from DNA. We also know that DNA use A, G, C, T, ...
2
votes
2answers
360 views

What kind of beetle is this (2cm long, brown, “V-shaped” body)?

I've seen this brown beetle, which was about 2cm-3cm long (I guess) near Loch Lomond (Scottland) a few days ago. What kind of beetle is it?
4
votes
1answer
3k views

How does this headless fish still move?

There is a (VIDEO) on Facebook where the fish starts to flail around despite no heart or internal organs. What causes the fish to flail around the way it does? My theories: It is because of ...
4
votes
2answers
128 views

Which primates can swim underwater?

I saw a documentary awhile back and they were profiling a certain species of monkey which regularly swims underwater. I can't recall which species it was but they said it was rare for primates to do ...
2
votes
1answer
53 views

Why the length of UTR in the genomic sequence of gene X is too much longer than the same region in the corresponding Refseq mRNA sequences?

I'm browsing in the UCSC genome browser and found that the UTRs length of KIAA0040 gene in the genomic sequences is too much longer than the corresponding the Refseq mRNA sequence. In fact, the total ...
1
vote
1answer
24 views

What is the convention for indicating genomic primer sites?

What is the most common notation for indicating genomic binding sites of primers relative to an ORF? For instance, if I want to indicate a primer like so: ...
1
vote
1answer
63 views

What is difference between several mRNA RefSeq for one gene?

I'm looking for mRNA RefSeq sequences for a given gene in the UCSC genome browser by searching gene name "HLA-F" in human (scroll to Refseq genes section). There are several RefSeq mRNA for the gene, ...
1
vote
0answers
43 views

Can a brown recluse bite lead to diabetes?

Ok, I realize that this question may seem crazy at first glance. I would agree. Also, this does deal with a medical diagnosis, however, as I'm more concerned with the explanation behind it, I figured ...
1
vote
1answer
51 views

Can someone die from pain? [duplicate]

I'm a CNA and I just got home from a very long day at work. I just started this job and I got hurt at work last night which made me wonder can enough pain cause death (without suicide)? I think the ...

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