5
votes
3answers
109 views

What makes DNA sticky-ends sticky?

When restriction enzymes jaggedly cut double stranded DNA it results in so called sticky ends. What is the substance that makes the DNA sticky?
3
votes
2answers
109 views

What is the scientific opinion on plus lens therapy?

Plus lens therapy is the theory that most myopia is environmentally triggered and that conventional corrective minus lenses increase nearsightedness over time. Thus the hypothesis that applying plus ...
1
vote
0answers
53 views

To define the total Resistance of Systemic Blood Circulation

Some systems are in parallel. For instance, the resistance of lungs $R_{\text{lungs}}$. Assume here for simplicity that these parallel systems can be handled linearly. We also consider only Total ...
1
vote
1answer
33 views

Clonidine's adrenonergic nature?

I am little confused here. I used the term adrenoagonist and sympatholytic to describe the compound. However, my teacher says that the correct term here is adrenomimetic -term. My understanding of ...
2
votes
1answer
63 views

Fast standing and the heart's insufficient accommodation of the increased venous return

Assume you are 45 minutes on the supine position. Furthermore: you stand all of a sudden and fast and without sympaticus activity. The venous return (smooth musculature of vessels) accommodates ...
3
votes
2answers
253 views

Why does methylation not occur in viral DNA?

Why does methylation not occur in viral DNA? Can viral DNA undergo the process of methylation? If not then why does this process does not occur in viruses?
4
votes
0answers
46 views

Why do some internal organs regenerate?

I have been reading about the human liver and zebra fish heart muscle having the ability to regenerate. It seems to me that these organs have very little chance to become damaged or worn out. At the ...
1
vote
1answer
69 views

Possibility of Human cloning

Technology has evolved so much as to clone animals. But, what about human cloning? Is it really possible to create humans in lab? Or is it that technology has not advanced to that level..? Why cant ...
2
votes
1answer
67 views

What causes a polypeptide to fold into an Alpha helix over a Beta pleated sheet

I know how they fold, but what causes some polypeptide chains to preferably fold into Alpha-helix rather than Beta-sheets (or vice-versa). What force makes it fold into one conformation over the ...
3
votes
1answer
42 views

Do invasive species survive in the long run?

If an invasive species preys on native species, spreads widely and becomes dominant, should it not become extinct soon enough because of a lack of food?
0
votes
0answers
37 views

From a list of protein accessions, how can I retrieve DNA sequences from GenBank?

I have 1000's of protein sequences in FASTA/their accession numbers. I want to go back into the whole genome shotgun database and retrieve all DNA sequences that encode for a protein greater than or ...
1
vote
0answers
34 views

Modified Mendelian Ratios Problem

I'm studying modified Mendelian ratios. I've got a theoretical problem that I'm stumped on: A species of bird may have normal wings, or a mutation that causes frazzled wings. I do 15 identical ...
5
votes
1answer
93 views

What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?

Reposted from Quora The Kozak sequence is shown to be important in translation initiation, and the A-site is the anti-codon recognition site. If the distance is not known, how could I find out what ...
7
votes
1answer
73 views

How significant is RNA degradation with removal of cap/polyA's in eukaryotes, or UTR's in prokaryotes?

Question is rather self-explanatory, but segmented into two parts. I'm attempting to make use of a repression system that employs cleaving RNA at specific areas with ribozymes with the intent of ...
1
vote
1answer
183 views

Is whey-protein folding an example of micelle in water? If not, what is it? [closed]

I can find a lot of information about protein folding in water but not about whey-protein-folding in water that body-builders use with filtered shakers. Without filters, you get protein balls -- is ...
1
vote
1answer
54 views

Plant tissue culture [closed]

Suppose there are two cases : a) A seed being embedded in an Agar media in a test tube, and then kept under controlled conditions. b) An individual cell being extracted from the parent plant ...
1
vote
1answer
33 views

Degenerate primer design for DIG in situ hybridization

New to molecular and have learned to design primers from google/youtube so any info would help Would someone be willing to share their protocol for degenerate primer design? Breakdown: Trying to ...
4
votes
1answer
31 views

A few questions regarding immunology [closed]

I know that there is a variable region on antibodies which can recognize a wide variety of antigens, and that germinal centers create more "fit" antibodies to respond to an infection. So I was just ...
1
vote
1answer
23 views

To understand synthesis of e-NOS and n-NOS with respect to Ca and calmodulin

I found this sentence in my notes interesting E-NOS and n-NOS are Ca-calmodulin dependent ferments. They are synthesized in small concentration as a response to physiological signals which ...
1
vote
1answer
39 views

Low copy numbers of plasmids

I have a plasmid with the P15A origin which apparently has a low copy number (see here). This would explain why my purification yeilds for subsequent digestion are low (gel shows the plasmid after ...
2
votes
1answer
27 views

Does amylolysis occur in vegetables if stored below 0⁰ Celsius?

Assume that we have a plant, e.g. kale, which is prone to amylolysis if not harvested during a frost period. If it is harvested and put in a household freezer, such that it is frozen solid slowly ...
1
vote
1answer
109 views

Energy flow in an ecosystem

What are the mistakes in here? The image below shows a part of the energy flow diagram in an ecosystem. 1. Part P of the biomass of the primary producers does not get consumed. Give a concrete ...
2
votes
1answer
49 views

Fusion of neuron with a S phase cell

There was some experiment in which a S phase cell was fused with other cell and the other cell also began to replicate DNA. Would the same happen if fused with nerve cell ? Why or why not?
2
votes
1answer
48 views

Duration of selection and survival of the fittest

I feel there is a part of the theory of evolution I'm not understanding correctly or maybe I'm missing it, but would like cleared up. From what I understand, over time random mutations occur which in ...
7
votes
3answers
68 views

If my brain lost all of its activity for a moment, would my memory be gone too?

I can't remember which question it was, but in the past here, it was explained to me that memory is actively stored in the brain, rather than "physically". Meaning, memories are made up of the brain's ...
1
vote
1answer
31 views

Anesthetics, specifically inhaled anesthetics

I have had a look at previous inhaled anesthetics and many of them appear to be fluorocarbons. What could be the mechanism behind fluorine's anesthetic properties? Is it the specific bonding pattern ...
2
votes
1answer
41 views

What roles do cyclooxygenase, lipoxygenase, and epoxygenase have in signal transduction? [closed]

Besides oxidizing fatty acids to form prostglandins, leukotrienes, and epoxides, what other roles do cyclooxygenase, lipoxygenase, and epoxygenase have in signal transduction?
3
votes
1answer
33 views

What are limitations on the number of objects the eye determine at a glance?

Take two people Sam (average human) and Jack. Jack says to Sam "I'm going to show you some skittles (or other object) and I want you to tell me as quick as you can how many I'm holding." He opens ...
1
vote
1answer
62 views

(how) is it possible to choose the biological sex of a child? [closed]

I recently read of a study that investigated the ethics of child's biological sex choice in humans, and now I want to find out how this can/could be done. Does anyone know of scientific papers or ...
0
votes
0answers
24 views

How do you accurately measure anguiliform (eel) locomotion?

In one of my courses, we were discussing a paper by Muller and van Leeuwen (2006) which made a lot of comparisons the differences between carangiform (mullet) and anguilliform (eel) locomotion. While ...
0
votes
1answer
20 views

If gene differs between chromosome pair does individual get a mixture of protein shapes?

Do diploids such as humans end up with a mixture of two different shaped proteins where the appropriate gene is expressed differently in the two halves of a chromosome pair or is there some governing ...
0
votes
2answers
56 views

Purifying a linear plasmid after restriction digest?

I expressed a yeast vector in E.coli and purified about 13µg of it. I then linearized it using a restriction enzyme, and attempted to gel purify it. I attempted this twice. The gel showed a clear ...
0
votes
0answers
34 views

Are ipratropium and tolterodine parasympatholytic?

They are nonselective cholinoblockers and antimuscarinic. Other cholinoblockers of parasympaticus, which I know, are parasympatholytic such as atropine, butylscopolamine, trihexyphenidyl, titropium ...
3
votes
1answer
51 views

PSI-BLAST website algorithm parameters

http://blast.ncbi.nlm.nih.gov/Blast.cgi In this website, when I want to apply the psi-blast algorithm on a sequence, under the section of algorithm parameters , what does PSI-BLAST threshold mean? ...
3
votes
2answers
38 views

What does “chlorophyll photosynthesis peak” mean in relation to photon wavelength?

When reading about how green / leafy plants work, I saw that they have chlorophyll A & B, which allow the plant to use the energy from light by capturing and transforming. When reading about ...
4
votes
1answer
51 views

How to evolutionary explain menstrural cycles synchronization? [closed]

Sorry, I can not indicate the source, but I read that menstrual cycles of women who live in the same house over time sync. Is it true? How does it work?
4
votes
2answers
44 views

Can the human eye distinguish colors in the periphery?

In the back of my mind I have the idea that human eyes can't notice the color of objects in the far periphery, and that any subjective perception of colors is done by the brain that tries to fill in ...
0
votes
1answer
13 views

Differentiation of norepinephrine and epinephrine in indications

Norepinephrine is less beta2 adrenomimetic than epinephrine so more selective so less bronchospasm so may be therofore better in treatment of cardiac failure and different shocks. However, I am not ...
6
votes
4answers
256 views

Is human vision sensitive to frequency or wavelength?

In a vacuum, there is a one-to-one correspondence between light frequency ($\nu$) and wavelength ($\lambda$), ie. $\lambda=c/\nu$. But in a refractive medium, $\lambda=v/\nu$, so while the frequency ...
3
votes
2answers
110 views

How many centrioles/basal bodies are there in multi-ciliated cells throughout the cell cycle?

I thought there were only two centrioles per cell, that convert to the basal body at some point during the cell cycle. I also thought there's one basal body per cilium, so I'm not clear on where the ...
1
vote
2answers
119 views

Theranos blood test's Specificity and Accuracy?

I am studying the limitations of Theranos device. They have 238 documents in Google Patents Search (Inassignee as Theranos), here. Which patents are relevant for their device? They have 70 tests ...
2
votes
1answer
34 views

Molds and fungi usually found on book pages: identifying and preventing further spread

I know I can use bleach to kill most of the spores that live on my walls. I know I have to keep humidity below a certain level and temperature above another to prevent the spread of mold. I have read ...
0
votes
1answer
23 views

Is there a stem cell dye for education purposes?

I need a dye that will stain stem cells (for this purpose the stem cells will proquired from chicken bone marrow). This will be an educational hands on activity for young teenagers so ideally the dye ...
1
vote
1answer
17 views

Determining break/border of movement

A population given inhabiting an area. I can trace every individual and I've got the hypothesis there is a break in distribution like individuals born in the upper half of the area will rarely move to ...
12
votes
2answers
2k views

How was birth control realized in ancient days? [closed]

In ancient days, there were no birth control methods like condoms or contraceptive pills available. How did they control pregnancy?
1
vote
3answers
116 views

Is there an online gene library? [closed]

Is there an online library that includes all known genes in stored in their nucleotide form of all living organisms? Note I am not necessarily looking for a human only database. If such a database ...
3
votes
1answer
42 views

What is the most recent well-attested common ancestor of chimpanzees and humans?

Humans and chimpanzees are related and thus have a most recent common ancestor. Of course pinning down this precise point is essentially impossible, so I'm interested in close ancestors of this most ...
3
votes
2answers
2k views

Presence of MHC on red blood cells

Do red blood cells have no MHC? (I have often heard that they do not.) If so why are they not destroyed by immune cells?
2
votes
1answer
48 views

Autoimmunity and central tolerance

Where in the body are self antigens important? In terms of central tolerance and autoimmunity, but also in terms of T cell activation?
2
votes
3answers
106 views

Is there a stem cell/ biogel method exist for muscle and tendon lengthening?

Is there a stem cell/ biogel method exist for muscle and tendon lengthening? It is known that height surgery is limit to six inches with two surgeries and three inches each, it is also true that ...

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