0
votes
1answer
12 views

What does this sentence about angina mean?

I do not understand this sentence Angina can be treated ambulatory (in out-patient) in stable angina. I think ambulatory refers to ambulatory ECG monitoring. So I understand this that you can ...
4
votes
1answer
50 views

Which are mobile and immobile elements in plants?

I am confused with this element or nutrient classification in the plants, since some authors set elements like $\ce{S}$ and $\ce{Ni}$ as mobile and other as immobile elements (Citation 1, 2, 3, 4, 5). ...
3
votes
1answer
56 views

Walk me through microsatellite markers and PCR

Three polymorph microsatellite markers are used to try and narrow down the location of a disease locus, with the use of PCR with 2 flanks on each side of the actual polymorphic area. The PCR ...
3
votes
1answer
38 views

Gene frequency problem

1/330 of white people in south Africa have a disease called Porphyri. What is the frequency of the gene if it is inherited autosomal recessive? This one I understand. The genotype frequency is $q^2 ...
2
votes
2answers
48 views

How do roots obtain nutrition?

I have a city garden (see picture below) and yesterday I was nurturing it and I pulled up a small plant. I saw its root system and laid it in the sun to dry a bit and then scraped of the rest of the ...
1
vote
1answer
34 views

Which tree's root can grow the longest?

In a recent TV documentary it was said that a particular tree root could grow miles long. However, I forgot the name of the tree and I don't remember the name of the TV program as well. I searched ...
5
votes
1answer
38 views

Independent contrasts with non-homologous traits

I have morphometric measurements for a trait across various animal taxa, and would like to study the relationship between the size of this trait, and the animals' body size. In theory, I can account ...
1
vote
1answer
18 views

How is magnet based bead capture used for indexing-first ChIP-seq?

The context for this question can be found in this paper by Lara-Astiaso et al. Here, to study H3K4 trimethylation in various cell types, they sort cells sonicate chromatin immobilize chromatin on ...
5
votes
2answers
148 views

Transformation of E.coli cells

As a team of undergraduate researchers we are looking to transform two genes of interest into our competent E.coli cells. Both of these genes are in separate plasmids and we would like to put them ...
2
votes
1answer
46 views

Is the golden ratio of anatomic measurements universally beneficial for sexual reproduction? [closed]

It is often claimed, in popular media, that the golden ratio (Fibonacci series) in for example some face measurements is aesthetically (and hence reproductively) attractive with respect to the humans. ...
2
votes
0answers
37 views

Write the haplotypes of the family

I'm doing old exam assignments to prepare for my finals on Monday and I've stumbled on one assignment that I'm not sure how to tackle. A family with 2 children is examined for cataracts using PCR ...
1
vote
1answer
38 views

Dynamic mutation and huntington's

I read that Huntington's is a disorder caused by dynamic mutations in the DNA, which means that a triplet sequence of DNA changes from generation to generation. Say we have the sequence ATGATGATGATG. ...
5
votes
1answer
69 views

How are behaviors genetically coded in animals?

I am curious as to how complex behavior is passed down genetically? For example, how is the building of a web genetically coded in a spider? And how is the complex behavior of constricting prey coded ...
4
votes
2answers
43 views

Flower identification (England, late May)

I took this photo near Stratford upon Avon by the side of a road. Can anyone identify this flower?
0
votes
0answers
27 views

Primer heterodimer problem

I design my primers in Primer3PLUS and analyze them in Oligoanalyzer3.1, A big problem that I have this is that I got very nice output in NCBI-Primer BLAST for their specificity but a very negative ΔG ...
3
votes
1answer
379 views

What is the name of this flower? The photo was taken in the Okanagan Valley, British Columbia

This little flower was growing on dry ranchland in Kelowna, it flowered in April:
6
votes
1answer
286 views

Can someone identify this plant?

Can someone identify this plant? It was growing under my 2 year old's climbing wall / play house thing. I live in West Virginia, USA and it's currently June 3rd (if that helps from a climate ...
0
votes
1answer
34 views

Are Humans Considered Keystone species? [closed]

Do humans have a huge impact on the ecosystem ? is it possible for other animals to live without us ?
3
votes
1answer
62 views

Do humans have skin cells that trigger oxytocin release in the brain?

I was reading an article by Montgomery on the therapeutic effect of pets (Boston Globe, January 12, 2015), and I found this quote: All animals appear to have cells directly under the skin that ...
2
votes
2answers
67 views

What is the difference between a spore and a cyst?

Last year I read a course in biology. During that course I learnt that Protozoa like Lamblia intestinalis or Entamoeba hystolitica form cysts in unfavourable conditions. This year I read a course in ...
0
votes
0answers
18 views

Help with STRING database

How can I generate a graph on the STRING web server for physical interacting proteins only? I know we could extract the data and use Cytoscape to create a graph, but I would prefer to have a graph ...
4
votes
1answer
60 views

How can electric fields inhibit tumor growth?

In MIT's Technology review it is explained that electric fields can cause dividing cancer cells to explode, while these fields have no significant impact on non-dividing tissues. The original research ...
1
vote
1answer
15 views

What's a segmented copy number profile

I am studying copy-number variation. I am reading C. H. Mermel, S. E. Schumacher, B. Hill, M. L. Meyerson, R. Beroukhim, and G. Getz, “GISTIC2.0 facilitates sensitive and confident localization ...
1
vote
1answer
39 views

Background correction when reading ELISA with TMB substrate

I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
4
votes
3answers
80 views

What is a genetic marker?

In DNA sequencing and analysis, what is a genetic marker? I've heard that microsatellites are genetic markers? Those are repetitive strands of bases such as GCAGCAGCAGCA etc. Why are they markers and ...
2
votes
0answers
44 views

Differing number of “things” in mammals [closed]

I'm not a biology expert, but I have often wondered about the following facts of life: All mammals have precisely 5 fingers on each of our 4 extremities. Granted, some may not be seen, but the bone ...
4
votes
1answer
199 views

Measuring body size in flies/insects

"Mesothorax length (the distance from the tip of the scutellum to the most anterior part of the mesothorax)" From Bergland et al 2008. Would that be this distance (green line)? If not, how ...
2
votes
1answer
70 views

From a computer science perspective, how is DNA compared for various purposes?

I am very interested in privacy preserving technolgies, such as Microsoft PINQ and would like to see if this is applicable to DNA comparison. Given that I don't have a background in biology, I ...
8
votes
4answers
70 views

Are there constraints on where intron/exon boundaries occur with respect to the triplet codons of the reading frame?

I did a quick search (here and elsewhere) and couldn't find anything on this subject. If all introns in a given primary transcript were spliced out in the same way, then this wouldn't matter. But ...
0
votes
1answer
15 views

Will tagging a protease with HlyA for secretion inactivate the protease?

We are looking to secrete the protease proline iminopeptidase by attaching the secretion signal HlyA to the protease in a gene circuit. Since the natural HlyA secretion sequence in Escherichia coli ...
2
votes
2answers
107 views

Value of mathematical models in biology [closed]

Sorry if this is too much of a soft question or if it is too broad for this site. As a person who has much more experience with math than biology, I've always been really interested in the ...
5
votes
1answer
51 views

Are there eukaryotes with fully working mitochondria but without mitochondrial genes?

Are there eukaryotic organisms with fully working mitochondria that do not have genes in the mitochondria? By fully working, I mean ones which can carry out oxidative phosphorylation, unlike reduced ...
2
votes
1answer
38 views

What is the distinction between the terms detrivores, decomposers, saprotrophs and saprozoic organisms?

All of them feed on dead and decaying matter (detritus). Detrivores and decomposers are distinct, as it says on Wikipedia, in the fact detrivores consume macroscopic clumps of detritus while ...
1
vote
1answer
32 views

Why is Electrical Cardioversion contraindicated in AF and Digitalis Poisoning?

I am thinking why electrical cardioversion is contraindicated in atrial fibrillation with digitalis toxicity/poisoning. Cardiac digitalis is also called digoxin and cardiac glycoside. It is ...
3
votes
1answer
30 views

Why do epithelial cells arrest in response to serum?

Primary epithelial cells, for example human mammary epithelium, fail to proliferate (arrest) in serum-containing medium. Therefore, a common growth medium for epithelium contains pituitary extract ...
7
votes
3answers
782 views

Why is triclosan not considered an antibiotic?

Triclosan is a chemical often referred to as a "biocide" instead of an "antibiotic". However, its mode of action seems to suggest that it is an antibiotic. Triclosan binds to bacterial enoyl-acyl ...
1
vote
0answers
18 views

Would it be possible to regulate proper phosphorylation so the UPR wouldn't initiate a reactionary cell death in important cells?

In studying the correlation of folded versus unfolded proteins and their impact on neuro-degeneration, it looks like improper phosphorylation in the chaperones (at least, in part) causes the ...
9
votes
1answer
847 views

How to prove that pork meat has parasites

I suspect that the pork sold at a certain shop is infected with the parasite pork tapeworm (Taenia solium). Is there a way I can prove this without special equipment? Would it work to leave the raw ...
2
votes
4answers
58 views

Do I need to serially dilute E. coli cultures for optical density measures?

I am expected to track cell growth by measuring the incubating culture's cell concentration every 30 minutes or so. So my questions are: Why do I need to do serial dilution (assuming that I do)? Why ...
4
votes
0answers
19 views

Non-cumbersome respiration sensor for sleep study?

I'm working on a project where I collect ECG signal from multiple volunteers, derive ECG derived respiration(EDR) from the timestamped ECG signal and calculate the cross correlation between EDR and ...
5
votes
1answer
28 views

How do nuclear membranes form during Telophase of Mitosis/Meiosis?

During Telophase, homologous chromosome pairs reach the poles of the cell with the help of microtubules. From there, nuclear membranes form around each new set of chromosomes. My confusion originates ...
2
votes
0answers
51 views

CRISPR-Cas Systems

In the context of the bacterial systems (not the gene editing tool), I was wondering what happens to the foreign DNA after the Cas proteins have created a new spacer. It is really not clear to me, ...
4
votes
1answer
21 views

Proline Iminopeptidase v Proline Aminopeptidase

We're an undergraduate independent research team and we are having trouble purchasing commercial proline iminopeptidase as it is unavailable on Sigma Aldrich and very expensive on other websites. We ...
2
votes
0answers
28 views

What is effectiveness of methyl-alcohol as sporicide?

Methyl-alcohol is the weakest alcohol in terms of bactericidal properties. However, I have read that it also works as sporicide. What is the effectiveness of methyl-alcohol in killing the spores of ...
6
votes
1answer
60 views

How can octopus walk if muscles can only contract?

From this video it is evident, that octopus can make tentacles push: https://youtu.be/zaE-LwDowcU How is it possible, if it is said, that muscles can only contract? What types of muscles can ...
2
votes
1answer
32 views

Vegetable Cellulose

I'm a pharmacy student working on my thesis. I just want to ask the probable sources of vegetable cellulose found in vegetable capsule? Note: I'm referring to the capsule itself not for its possible ...
4
votes
1answer
20 views

How do Mockingbirds find each other during mating season?

Since mockingbirds imitate songs of other birds, how do they find each other during mating season? How can they tell it's a mockingbird singing, instead of other birds? Is there something in their ...
0
votes
0answers
35 views

Can these “Promega Wizard genomic DNA Purification kit” be used for samples not stated directly?

I am not sure if this is a duplicate of my previous question, it is somewhat related, so sorry if I misunderstood. The question is, can i use the above kit for samples like hair, fingernails, etc? ...
1
vote
1answer
42 views

Why is the gene regulation in eukaryotic cells needs multiple level of control than in prokaryotic cells?

That "eukaryotic cells are more complex" and "compartmentalized" are used to justify the need of more level of control of gene expression. I get the basic idea but can't convince myself why complexity ...
3
votes
1answer
48 views

Why increasing the vector concentration does not increase the effeciency of bacterial transformation?

I was reading some old description of the protocols used for the transformation of bacterial cells. In the description I read that the transformation works best with low amount of DNA, and if we ...

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