5
votes
2answers
296 views

Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
5
votes
2answers
104 views

Creating a cell, not from another cell. Will it be possible?

If some time in the future, we can know exactly what a cell (for example simple prokaryote bacteria) contains, (I mean, exactly which molecules, the shape of them, the density of each, everything), ...
4
votes
1answer
403 views

Is there a relationship between Melatonin, Norepinephrine and depression in humans?

I'm reading a booklet on melatonin published in 1996, titled "Melatonin and the Biological Clock". This particular paragraph caught my attention and I would like to better understand what it means: ...
2
votes
0answers
184 views

What specifically allows alpha-complementation in beta-galactosidase?

I'm familiar with the use of alpha-complementation of beta-galactosidase with the pUC alpha-peptide and the M15 lacZ gene product, and would like to hypothetically apply alpha-complementation in other ...
6
votes
3answers
148 views

What reasons allow for women to outlive men?

It is a well-known fact that women tend to outlive men. I often hear people unscientifically stating that men generally generally die younger because of the higher stress encountered in their work ...
8
votes
2answers
163 views

How are antibodies designed?

Antibodies have the ability of recognising highly specific peptide sequences and bind it at their antigen-binding site. This ability is harnessed as a tool in research to purify target structures in ...
7
votes
1answer
260 views

Does DNA contain information beyond protein synthesis?

It's well known that genetic information is stored in DNA. As far as I know, DNA only has information at the protein level. What about higher levels, such as organelles, cells, tissue, organs? Is ...
3
votes
2answers
132 views

Why is most tissue cellular?

Most tissue is comprised of cells. Why? It would seem inefficient to have so many individual nucleus, membranes, etc.? Specifically: Not all tissue is cellular. Much tissue is extracellular matrix. ...
7
votes
1answer
192 views

When giving blood, what is the specific Iron measurement they take?

I went to give blood the other day, and they take an "iron" measurement prior to actually drawing the blood. My level was 16.4 -- but what specific type of iron were they measuring? I've heard of ...
7
votes
1answer
59 views

How well does it actually work to surgically reroute the optical nerve?

Two publications, Roe et al, 1992[1] and Metin & Frost, 1989[2], describe results pertaining to the ability of a region of cortex to process information from a different sensory mode than the one ...
6
votes
1answer
229 views

Influence of alcohol on brain cells

As I am not related to biology, I would appreciate if you can keep your answers as simple as possible. My question is about the influence of alcohol on the brain. As far as I know, drinking alcohol ...
6
votes
1answer
132 views

What is this crow eating, and is it a common part of the corvid diet?

Here's a picture (by Rob Curtis) of a crow carrying and eating the corpse of what looks a bit like a small hawk or falcon: Other pictures clearly show the crow is eating the dead bird. This image ...
4
votes
1answer
98 views

How does sex differentiation work in Paracerceis sculpta, the sexually tetramorphic isopod?

Paracerceis sculpta is a marine isopod species known for its unusual reproductive strategy: female: medium-sized; lives in harems run by an α male α male: large; keeps a harem of females β male: ...
6
votes
2answers
416 views

Methods of nuclear transfection - nuclear transport

I am reading through the ENCODE papers, which is taking me well out of my comfort zone in terms of modern laboratory techniques. At the risk of asking a question which may well be thoroughly answered ...
7
votes
1answer
68 views

Which poison makes seastars inedible to possible predators?

In the new citizen science project (see: Sea Floor Explorer), numbers of seastars, scallops, crustaceans and other animals are counted. Already one can see a heavy bias in favor of seastars, both the ...
3
votes
2answers
90 views

Have proteins been observed to come into existence through mutations and natural selection?

What is an example of a functional protein that has been observed (in real time) to have come into existence through mutations and natural selection (not through an existing one being made defective). ...
4
votes
2answers
115 views

Ultrasound during pregnancy

In a hospital I can see a long queue of pregnant women waiting for their turn for ultrasound. Is it safe to go through the ultrasound during pregnancy, especially during last few weeks? Is ...
2
votes
1answer
89 views

Which cells will pass cancer to offspring?

Each of these types below contains a DNA mutation. Which type(s) will affect the children of the individual whose cell it is. Red blood cell T cell Skin (epithelial) cell Neuron from the brain Sperm ...
2
votes
1answer
61 views

Age-associated neurodegenerative diseases

Age-associated neurodegenerative diseases encompass Alzheimer's Disease and Parkinson's Disease. What other neurodegenerative diseases could be described as age-associated? Multiple Sclerosis? Brain ...
9
votes
1answer
1k views

What determines testosterone exposure in womb, baby's genetics or mom's body?

I read this article which has the following quote: The ratio between index and ring finger is believed to be linked to exposure to the male hormone testosterone in the womb. And I wonder what ...
9
votes
1answer
993 views

Do fish break a water molecule to absorb oxygen?

How do fish separate oxygen from H20 & consume it? Do they break the water molecule and absorb the oxygen only?
1
vote
1answer
3k views

Which enzyme catalyzes transcription and which translation?

My attempt: Transcription - RNA polymerase Translation - aminoacyl tRNA synthetase I know that the ribosome almost runs translation, but I don't think ribosomes are enzymes, which is why I went ...
5
votes
1answer
192 views

How are DNA polymerase error rates measured?

It is well known that the first DNA polymerase, Taq, is quite error prone. Newer generation commercial enzymes that have either been isolated from different thermophile species or have been improved ...
8
votes
1answer
598 views

Digital Genomic Footprinting for ENCODE

I'm reading over the ENCODE Nature papers, and one of the papers referred to is "Global mapping of protein-DNA interactions in vivo by digital" by Hesselberth et al[1]. Genomic footprinting is a ...
11
votes
2answers
177 views

Why do most breast cancers occur in women?

According to Korde et al. (2010): Male breast cancer accounts for less than 1% of all cancers in men and less than 1% of breast cancers. This raises the question: Why do most breast cancers ...
7
votes
1answer
378 views

How to prevent protein precipitation?

I'm trying to label a protein with fluorescent dye (TMR succinimidyl ester), but having trouble getting the protocol to work. The protein seems to be stable in distilled water at a the recommended ...
6
votes
1answer
197 views

Models of quorum sensing for multi-agent systems

Quorum sensing is a system of stimulus and responses correlated to population density that is used by bacteria to coordinate gene-expression. I am looking for a simple computational/mathematical model ...
2
votes
2answers
197 views

Is consuming sugar with food as beneficial as consuming glucose (~half of the amount of sugar)?

A few years back I heard at biology class that sugar's molecule consists of half the number of elements of a glucose molecule. So not counting the energy needed to transform two sugar molecules in one ...
9
votes
1answer
817 views

Why are some berries poisonous?

In my understanding, the evolutional function of berries is to be eaten and pood out somewhere else, so that the seeds of the plant spread. Is this so? Then why are some berries poisonous?
5
votes
0answers
98 views

Pharmacologically, can tricyclic antidepressants have a side-effect profile similar to neuroleptics?

Torticollis (wryneck, cervical dystonia) is a neurologic movement disorder causing involuntary muscle spasms in the neck. Often, neuroleptics can cause such a side effect. I'm wondering if this ...
3
votes
2answers
68 views

Microbiome Data

How is it possible to access microbiome data like that found here? I'd like to perform analyses on similar type of data, but cannot find OTU data like that at the Human Biome Project. Thanks for any ...
3
votes
2answers
359 views

Is there a physiological difference between love and infatuation?

I find that people generally distinguish between love and infatuation. Is there a physiological difference between the two? Or are they biologically identical, and the difference is circumstantial?
6
votes
3answers
568 views

How exactly are game theoretical evolutionary models described during implementation for computer simulations?

When a biologist or a layman tries to reason the evolutionary explanation for something, they would simply use English with some math thrown in (for a random example, pick any explanation out of "The ...
3
votes
1answer
855 views

Can a person become addicted to tea?

Can one become addicted to tea like coffee addicts or smokers? I heard there are some common substances in tea and coffee, do they cause addiction?
5
votes
1answer
104 views

Phenotypic Variation of cattle - looking for academic sources

Can someone please point me in the direction of a good academic article on the following: What are possible sources of phenotypic variation of different 400 day weights of cattle? Furthermore how ...
3
votes
1answer
133 views

What is the relationship between Homocysteine and Norepinephrine metabolic cycles?

I've been researching neuromodulators and have taken a look at the Norepinephrine/Noradrenaline system. The diagram of biosynthesis of Norepinephrine is presented in the wikipedia article: ...
8
votes
2answers
227 views

Does human digestive system benefit from eating same kind of food consistently?

Here's the reason why I'm asking: I've heard that it takes some time for pets(cats/dogs) to adjust to a different dry feed, where the new feed has to be mixed with the old one, slowly replacing the ...
2
votes
3answers
79 views

Is DNA mutation locally energetically stabilizing the DNA molecule

I am no biologist, but as a physicist, a spontaneous mutation (seen as a chemical transformation) should lower the energy of the system, at least locally. So I wonder if any research has been done ...
7
votes
3answers
203 views

Is there any documented evidence for suicide among vertebrates other than humans?

Is there any documented evidence for suicide among vertebrates other than humans? Lemmings not accepted !
3
votes
2answers
128 views

What is the species of these mushrooms?

The mushrooms are gilled with a light-brown cap. The stem is widened to the base. What species is it? Is it considered edible? They are found in mixed forest in Moscow, Russia. They grow in the ...
2
votes
1answer
166 views

How to learn DNA Origami

Can you tell me is there any good softwares and tutorials which can be used to learn DNA Origami. I am new to this and want to learn from basics. Advance thanks for your help edited: INSILICO
4
votes
1answer
51 views

Does retinal detachment happen more frequently at night?

My understanding and experience is that during the night photoreceptors are less tightly coupled to the retinal pigment epithelium. Does this make it more likely for a retinal detachment to happen at ...
3
votes
2answers
260 views

Why is the frog genome so much larger than a fish's?

As we have heard in the summaries of the human ENCODE project, 80 per cent of junk DNA appears to have an essential function. Many fish have a genome with only one tenth the size of a usual vertebrate ...
6
votes
2answers
390 views

What distinguishes “coding” and “noncoding” DNA from each other?

I've been reading a bit about "junk DNA" and how much of our genome consists of this "non coding DNA" in comparison to "coding DNA". I'm just an interested layperson but I thought all combinations of ...
0
votes
1answer
373 views

Is breathing in deodorant fumes dangerous for my brain?

I noticed that I sometimes get dizzy when I accidentaly breathe in too much Axe. Also, I find the smell of alchohol rich deodorants quite unpleasant. But, I was wondering, is it actually dangerous? ...
5
votes
1answer
109 views

Mutation-immunity in Luria-Delbruck experiment

If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...
14
votes
2answers
1k views

Is there an advantage to linear chromosomes?

The DNA copying enzymes have a hard time working to the end of a chromosome. For circular chromosomes this is not a problem, since there is not a sharp 'end'. However, for a linear chromosome, without ...
4
votes
1answer
141 views

Is cell senescence in culture comparable to that in vivo?

A cell is 'senescent' when is has permanently left the cell-cycle. This can be caused by stresses, or by reaching the 'Hayflick limit' (the cell has reached its replicative lifespan, as defined by its ...
4
votes
1answer
129 views

How does “inheritance of methylation” of DNA and/or histones work?

What are the current models/ideas describing the mechanisms explaining inheritance of methylation on DNA resp. histone level? Is there evidance of this "setup" information being really ...
2
votes
0answers
70 views

Terminology question: the scope of an allele in an organism

Let us consider a gene FOO with novel type foo. If I were discussing an organism that has inherited foo in every cell during classical zygote formation, then I would ordinarily just say that the ...

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