1
vote
0answers
40 views

Protocol for immobilize oligo to solid [closed]

I'm learning about microarrays and understand that an oligonucleotide can be immobilized to a solid like a CD acrylic plastic by modifying the oligo. Does anyone have a good protocol for modifying a ...
1
vote
1answer
116 views

DNA Replication

I just wanted to understand the basic steps behind the replication of the lagging strand of DNA: Have helicase unwind it first DNA Primase lays down RNA primers in fragments, called Okazaki ...
1
vote
1answer
40 views

Can antimicrobial peptides be called antibiotics?

Antibiotic peptides are present in almost all multicellular eukaryotes. So can they be called antibiotics ? ( which are usually defined only for fungi and bacteria )
2
votes
2answers
89 views

What causes autism?

What causes autism? By this I don't mean what is to blame i.e. Vaccines, Gluten or Pharmaceuticals etc.. I mean what exactly is happening in the brain to cause the autistic behaviors such as little ...
2
votes
0answers
111 views

Good poly-A filtering rules or tools

I am aligning a large number of ESTs. It seems poly-A tails show in many different ways. In addition to occurring at the very end, they can be flanked by the cloning sequence one one end, or have ...
7
votes
4answers
427 views

Suitable introductory book on Bioinformatics for a computer scientist?

Do you have any suggestions on a suitable introductory text on bioinformatics for a computer scientist? Any recommendations with pros and cons of different books would be appreciated. I'm mainly ...
4
votes
0answers
65 views

What is the limit of time resolution in fMRI?

I was reading about a few studies on estimating functional connectivity between brain areas using fMRI signals. However, as far as I know that fMRI has a very poor time resolution, roughly in the ...
3
votes
1answer
134 views

Hearing and neurons- do ears have a sampling period?

From what I have read, outer hair cells in the human ear amplify incoming signals and inner hair cells "pick-up" the signals and generate action potential. However, neurons have refractory periods ...
3
votes
1answer
1k views

Does photosynthesis require only direct sunlight

I have learned throughout my basic science classes in high school that in the process of photosynthesis, plants absorb sunlight & produce food. They use carbon dioxide & water vapour. My ...
6
votes
3answers
317 views

Mitochondrial DNA and recombination

Firstly I could do with a brief description of mitochondrial DNA. How does the structure of DNA in mitochondria compare to animal DNA (for the sake of simplicity let's say human - some animals might ...
5
votes
1answer
158 views

Do thymine and uracil ever appear in the same strand of RNA?

Wikipedia says that "in RNA, thymine is replaced with uracil in most cases." I'm curious what are the cases when this does not happen? Does this ever occur in normal functioning, or is it an error? ...
2
votes
1answer
724 views

Difference between Bioscience and Lifescience

Are bioscience and lifescience the same things? I am a little bit confused about it. If not, then what is the difference between the two?
2
votes
1answer
129 views

DIY extraction and long term storage of human DNA sample?

What is the recommended DIY way of extracting and long term storing of human DNA samples to store at home and send for sequencing in years time? Considerations: Sample material: cheek swab, hair, ...
4
votes
4answers
395 views

What is the viability of Intelligent Design as a supplement to chemical abiogenesis and Darwinian Evolution?

First of all I am not endorsing Intelligent Design (Wikipedia link); I'm asking this because I (someone who does not have a background in biology, organic chemistry, or philosophy) got into a ...
1
vote
1answer
150 views

Can PolyA tails be within Expressed Sequence Tags?

Can polyA tails occur within (rather than at the end) of a sequenced tag? Consider, for example the following two sequences from NCBI: DY008075 ...
0
votes
1answer
58 views

Some question about materials for PCR

I'm planning to order oligo, polymerase, nucleotide, buffer for my first diy PCR experiment (now I have nothing), so I have some questions. How and how long I can storage them? I have only a fridge ...
3
votes
2answers
2k views

Why do we squint when tasting very sour things?

Sometimes while tasting a very sour thing (like tamarind, lemon etc.) our eyes squint immediately and involuntarily for a second, but a little later becomes normal again. Why, and how, does this ...
0
votes
1answer
329 views

How different is an introverts brain different from an extroverts?

How is an introverts brain wired differently than an extroverts? Just to give you an example. On an average, the processing that goes on in an introverts brain is greater than in an extroverts ...
0
votes
1answer
54 views

Carotenoid biosynthesis in yeast

Does Budding Yeast Sacchromyces cerevisiae produces significant amount of carotenoids? Have anybody estimated the ratio of flux going in branches 1. Cholesterol synthesis(via squalene) 2. Coenzyme Q6 ...
5
votes
2answers
200 views

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?
2
votes
2answers
223 views

Can a dNTP be built into a RNA strand?

DNA consists of deoxyribonucleotides, RNA consists of ribonucleotides. They differ mainly (apart from the uracil / thymine difference) in the sugar part, the deoxyribose and the ribose. Those two ...
0
votes
1answer
274 views

Confusion regarding photosynthesis and respiration in plants

During 24 hours there is a time in i.e. twilight when plants neither give oxygen nor carbon dioxide why is it so? This also suggests that neither of the two vital processes i.e. respiration or ...
3
votes
1answer
116 views

Method to recognize the age of a firebug from the pattern on its back

Can I recognize the age of a firebug from the pattern on its back? If yes, what pattern shows what age/stage? Below is what I extrapolated from Wikipedia, but is there a more precise scale with more ...
1
vote
0answers
57 views

Which are the most extreme artificial environments that lifeforms/ecosystems have adapted to?

I have heard of 'extremophiles', lifeforms that naturally thrive in all sorts of extreme environments. Is there any evidence to suggest that lifeforms are adapting to the extreme environments (such ...
0
votes
0answers
47 views

Are stable proteins tagged by ubiquitin? [on hold]

What would happen if an extremely stable protein was created because of some error? Would that protein be tagged by ubiquitin?
1
vote
1answer
39 views

What Effect would Cannabinoids have on the Acute Porphyrias?

I should be clear in saying that this question is NOT intended for personal medical advice. Rather what I am looking for is journal articles and/or books that touch on this subject because I think it ...
4
votes
2answers
609 views

How does ubiquitin recognize misfolded proteins?

Misfolded proteins are tagged by ubiquitin and then are destroyed by proteasomes. How does ubiquitin know which protein to tag?
5
votes
1answer
60 views

Selective Androgen Receptor Agonist

I'm looking for an inducer that strongly activates the androgen receptor, but not the glucocorticoid receptor that is not DEA regulated. I know that SARMS (Selective Androgen Receptor Modulators) are ...
3
votes
1answer
1k views

What biological constraints are there on how large a spider can grow?

Spiders fascinate me, but what intrigues me the most is just how big some spiders can get, which leads me to my question. What biological constraints (if any) would limit how large a spider could ...
10
votes
1answer
213 views

What are the functions of magnetic bacteria?

I'm trying to understand why are bacteria "equipped" with magnetosomes and why/what for do they need such organelles.
3
votes
1answer
1k views

What insect is this? (Black body two orange lines in its back and six legs)

I found this today in the library while studying. I am very curious to know whether this particular insect has a name that I can look up and find more information about: Full length is almost one ...
1
vote
1answer
211 views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
5
votes
3answers
128 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
2
votes
3answers
105 views

What do cells do when they aren't creating proteins?

I've always thought that the majority of the "work" in a cell is protein production, until I read the following. The Wikipedia article on the central dogma of molecular biology states this: 80% ...
1
vote
2answers
316 views

Does osmosis occur when we have two different substances?

Assume that we have a semi-permeable membrane with water on both sides. First, straightforward, case: On the right side, we have a concentration of the substance A, and on the left side, we have pure ...
1
vote
2answers
8k views

What are the functions and differences between axons and dendrites?

My textbook doesn't do a very good job of pointing out what the differences between the two are. It basically mentions axons only in the same breath as the synapse (that synapses are the endings/tips ...
4
votes
2answers
392 views

Why are enzyme-catalysed reactions slower at lower substrate concentration?

Suppose I'm using 200 nmoles of enzyme and 2 mmoles of substrate. The enzyme should be saturated but if I use 50 mmoles of substrate, the reaction will be faster. Why? I just can't get it! Even at ...
0
votes
3answers
122 views

Pump to separate protein/DNA instead of electrophoresis?

I know that DNA molecules or proteins can be separated in electrophoresis because the electrical charge is used to pull the DNA through the gel. So instead of using electrical, can I using physical ...
1
vote
1answer
92 views

How potent is the venom from the Australian platypus?

The Australian platypus, is a marsupial with a unique feature - in that the males of the species have venom sacs around their hind-claws. My understanding is that this venom is not lethal to humans, ...
2
votes
0answers
110 views

Coiling of chromatids during cell division

What is exactly coiling of chromosomes? I just heard about the names i.e paranemic, plectonemic, orthostichious, anorthospiral. I have ecaxtly no idea of what phenomenon is this. Also what type of ...
4
votes
2answers
7k views

What happens to the holes in the skull where teeth were developing?

In Skeptics.SE, a question regarding the skulls of children arose. Depicting a scary skull where the permenent teeth are "hidden" inside the jaws and the milk teeth are in the their place. After all ...
1
vote
0answers
58 views

How to ligate DNA to an RNA molecule

Is it possible to ligate a small DNA molecule to mRNA? I was reading a protocol from Current Protocols in Molecular Biology and one of the procedures involve ligating a DNA molecule to mRNA using a ...
0
votes
2answers
133 views

What do you call the non-Wild Type allele?

Can I have a list of suggestions, such as the mutated allele, other allele, etc. ? Are there any blatantly missing ones on my short list?
2
votes
1answer
67 views

Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
3
votes
1answer
827 views

What is the exact principle of capillary isotachophoresis?

I know it's a type of capillary electrophoresis, but I don't get how does the separation happen exactly.
0
votes
1answer
139 views

How do you remove DNA/RNA from plastics?

I want to know how to make plastic containers free of DNA & RNA? this is just for knowledge
3
votes
2answers
76 views

Why not use Cu(OH)2 as a dye for protein electrophoresis?

As I know, $Cu(OH)_2$ $+$ Protein $\rightarrow$ Purple. So why not use $Cu(OH)_2$ as dye in protein electrophoresis?
3
votes
1answer
140 views

Why Is The Toughness Of Skin Different On Different Parts Of The Body?

My cat was licking my arm with his sandpaper like tongue. It hurt and the area he was licking was slightly smarting afterwards. However, when he licks the palm of my hand the feeling is rather ...
5
votes
2answers
1k views

What is dental health like in animals?

Do animals suffer from tooth decay, such as cavities? Is it to the same extent as in humans, or do animal teeth decay more or less? If teeth decay in animals as well, are there natural strategies ...
11
votes
4answers
162 views

Is there any other function of DNA?

We all know that DNA acts as a genetic molecule. Does DNA have any other function in the cell other than being a genetic material and carrier of information?

15 30 50 per page