4
votes
1answer
221 views

Body size, what are the evolutionary trade-offs?

Background Considering just the "Kingdom Animalia" branch of organisms. It is clear that bigger does not necessarily mean better - there is large variation in body size... From the 94 µm long ...
4
votes
3answers
181 views

Why do we need deep sequencing?

Why do we need deep sequencing? Why cannot the sequencing technologies read all the nucleotides correctly at the first read? Sorry since this question is too trivial, I don't have a biological ...
1
vote
1answer
54 views

The reason why researchers usually use cell lines from “blast cells”?

What's the reason why researchers usually use cell lines from "blast cells" (so, immature, like lymphoblastoid cells) for measuring gene expression data? Is that they are growing up, which would make ...
1
vote
2answers
306 views

Blast a sequence against multiple databases

I would like to BLASTP a list of protein sequences in fasta format against multiple protein databases. Since I'm only interested in the first hit of each database, it is possible that BLAST result ...
0
votes
1answer
21 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
3
votes
2answers
200 views

Modern immunology textbooks

I am looking for recommendations to good textbooks introducing modern immunology. Review articles, preferably modern (post 2000) would also be useful. Please, include a brief comment with each book ...
1
vote
1answer
274 views

What are the differences between HPRD and BIOGRID databases?

What are the differences between HPRD and BIOGRID protein-protein interactions databases? What are their purposes? Why do we need two different databases? How is data collected into each one? How ...
1
vote
1answer
116 views

How is deviated nasal septum related to cold

I have a deviated nasal septum and I always suffer from common cold I was just wondering how a deviated nasal septum can be the cause for chronic common cold
3
votes
1answer
291 views

Is there a maximum pain threshold?

Is there a maximum threshold of pain a Human can experience, beyond which point there is no noticeable difference? I ask this question in part to better understand the definition of pain and its ...
5
votes
1answer
98 views

Could murder be modeled as an infectious disease?

Background "When swine flu hit the population it spiked in certain areas and tapered off in neighboring regions, it hits hardest where people have least protection and this pattern is more ...
3
votes
1answer
60 views

Gradual slowing breath, its effect on health

I've written a computer program which beeps, then beeps after 10 sec, then beeps after 11 sec, then beeps after 12 sec, etc. I tried the following "experiment" on myself: do only one breath between ...
2
votes
1answer
38 views

Calculation of saturation of carbon in surface waters

I've been looking at some journals lately that look at carbon emission from lakes where some lakes act as sources and other act as sinks. The paper that I have been looking at is ...
1
vote
1answer
137 views

Can anyone identify this caterpillar / cocoon?

This insect is about 0.5 mm in length, so it is very small. It doesn't have legs I don't believe, I am not too good at this but I don't know if it's a worm or more like a cocoon for something. I do ...
5
votes
1answer
114 views

By what mechanism can hypoglycemia induce fainting?

Hypoglycemia (Low blood sugar) is said to be among the potential causes of human-faintings. I was wondering by which mechanism this happens. Google search results are abundant; there are lists of ...
4
votes
1answer
137 views

Does conjugation support gene selection?

I have already posted this on chat but haven't got any response. A recent question on group selection stimulated me to ask this here. QUESTIONS: Why should bacteria conjugate? If we consider that a ...
2
votes
1answer
324 views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
1
vote
0answers
21 views

what are hyperbranched amplicons in DNA sequencing?

I am reading an article about single-cell sequencing: http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.2720.html And came across the concept of "hyperbranched amplicons". I googled for it but ...
4
votes
1answer
231 views

Time spent in phases of cell cycle

I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am ...
1
vote
3answers
169 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
2
votes
3answers
190 views

Bacterial cell lysis buffer used in proteomics procedures

What kinds of detergent-free bacterial lysis buffers exist? The proteins we're extracting will be later analyzed by LC-MS/MS, and we're looking for a lysis buffer that won't interfere with this ...
2
votes
2answers
127 views

Interdependency between organsims on our planet and its relation to ecosystem?

I am not a biologist but I just wonder if all species are interdependent. I mean if the tiger becomes extinct does it affect the ecosystem? If the mosquito or mouse become extinct does it affect the ...
3
votes
1answer
154 views

Empirical evidence for Group Selection?

A controversial concept in Biology "Group Selection", has caused confusion and conflict amongst scientists since the since the mid 1990s. The more general realm of study is termed the "unit of ...
1
vote
0answers
143 views

On the definition of gDCW (gram dry cell weight)

I need some clarification on the concept of gram dry cell weight. The unit acronym gDCW stands for gram dry cell weight. My interpretation is that 1 gDCW is equivalent to 1 gram of dry cells, but I ...
1
vote
1answer
2k views

Is it safe to look at infrared LEDs?

What happens if one looks directly at infrared LEDs? Sometimes I see this kind of LED at night as red dots and I'm also courious about it. I was wondering what would happened if one looked directly ...
3
votes
2answers
150 views

Are these cats calico?

Calico cats are cats that have 3 fur colors and are always female or males two X chromosomes. I've seen many cats that will have 2 fur colors (one of them is usually white) and another, third, color ...
1
vote
2answers
226 views

Is the SRY gene the sole responsible of biological male sex?

It's very well known that the Y chromosome is what determines maleness, but more specifically this seems to happen thanks to the SRY gene located on it. Some individuals have an XX karyotype, but ...
4
votes
2answers
71 views

Question about the expression of MHC

Is it possible to express both the MHC class-1 and MHC class-2 both in one cell?
10
votes
2answers
269 views

Why, specifically, does each generation, on average, improve upon the design of the species rather than degrade it?

In every non-life example I can envision, a copy of a copy is always a degraded or less pure version of the original unless some outside influence acts to correct the copy back toward the ideal ...
4
votes
2answers
87 views

Do all humans experience flatus regardless of their diet?

Flatus frequency is affected by diet, so I was wondering if flatus production solely depends on the food eaten, meaning that some people never ever release flatus because they don't eat any ...
2
votes
1answer
79 views

Determining sequence of oligoribonucleotide

Oligoribonucleotide X was treated with phosphatase (for removal of 3' and 5' - terminal phosphates), then with RNAase T1, which cleaves all phosphodiester bonds located in a 3' position of ...
0
votes
1answer
177 views

What are the universal laws of Nature concerning animals' behaviour? [closed]

I'm looking for very old (centuries) treaty or recent (20 & 21th century) research that state what some of the laws of Nature are, particularly concerning wild animals' behaviour. Such as; every ...
1
vote
0answers
1k views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
1
vote
1answer
147 views

Do genes play a role in the athleticism and shape of a person?

I am an Indian with a small thin stature with some deposition of fat around my belly.I was wondering if genes play a role in fat deposition in the body for Indian, Chinese, European or African people. ...
4
votes
4answers
283 views

Biodiversity is restricted by genome combinatorics?

Me and some friends are interested in opinions for the following: Conjecture The maximum number of species must be limited by the maximum combinatorial/permutational space that can be occupied ...
4
votes
1answer
114 views

Natural enemies boost deep evolutionary fitness?

"Two species that reduce one another's fitness on microevolutionary (short-term) timescales can increase each other's macroevolutionary (long-term) fitness" Opinions? Intuitive Examples: ...
4
votes
1answer
80 views

What makes alcohol harmful to mammals?

During the alcohol metabolic pathway, harmful by-products are made like acetaldehyde, hydroxyethyl, superox­ide anions, and hydroxyl radicals. How do these toxic compounds harm our tissues? Many of ...
5
votes
1answer
423 views

Could alcohol be used as an antibiotic?

I was thinking about how alcohol is used to sterilize, and I was wondering if it would be possible to use alcohol as an antibiotic? if it kills bacteria, couldn't (conceivably) alcohol in the ...
3
votes
1answer
633 views

Problem with bacterial transformation with electroporation

I have a problem with a bacterial transformation of a yeast gene that I can not solve. I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. I digest ...
1
vote
2answers
39 views

Regulation of the replication of mtDNA at embryonic level

While reading an article on mitochondrial inheritance I came across this link. The results state that mitochondrial DNA replication is regulated in different cells of an embryo at different levels. ...
1
vote
1answer
222 views

What is the most recent non-primate ancestors of humans?

If we go down the evolutionary tree of humans, (Homo sapiens, Homo erectus, ...), what will be the first non-primate ancestors?
3
votes
1answer
52 views

Are there cannibalistic k-selectors?

My understanding of r- and k-selectors does not go deeper than the wikipedia article. I understand k-selectors to be creatures that put tendentially more effort and energy into rearing their ...
0
votes
1answer
960 views

Are Italians genetically separated from other Europeans?

I was reading this Wikipedia article about the genetic history of Italy, and I found it interesting. There are however a few things that puzzle me, because they seem to contradict each other. For ...
3
votes
1answer
77 views

What's a replicate line?

The methods section inside a paper I'm reading make mention of replicate lines. Example: "We founded 10 replicate lines from a single clone". This is in the context of experimental evolution and ...
1
vote
2answers
1k views

Definition of “Niche Complementarity”

What does "Niche Complementarity" means? On this website they define "Niche Complementarity" as: The tendency for coexisting species which occupy a similar position along one niche dimension ...
1
vote
0answers
62 views

What are the ingredients of Pheromone Trap using for controlling Fruit flies of cucumber?

Pheromone Traps are used for fruit fly control. But I have no idea which ingredients or chemicals are used for preparing Pheromone trap
2
votes
2answers
3k views

Why aren't we allowed to refreeze previously thawed breastmilk in refrigerator?

https://www.breastfeeding.asn.au/bf-info/breastfeeding-and-work/expressing-and-storing-breastmilk Previously frozen— thawed in refrigerator but not warmed 4 hours or less (ie the next ...
0
votes
1answer
21 views

Order of events in hibernation

Arrange this in sequence : i. Heat loss exceeds heat production. ii.As body temperature falls, heat loss decreases. iii.Body temperature equals environmental temperature. ...
0
votes
1answer
22 views

Is ability to easily pollinate desirable?

In Mendel's crosses was it desirable for the plants to be able to cross-pollinate and self pollinate easily ?
0
votes
0answers
29 views

Where can I get yeast mutants? [duplicate]

I want to use some yeast mutants to conduct gene functional analysis, and the gene was isolated from plants. Can anybody give me some information regarding the websites or agencies from which I can ...
3
votes
0answers
45 views

Why is this dry pepper fruit still “powered”?

Check out this photo of my pepper plant this cloudy morning: (click for full res) Usually (and this can be seen on 2 and a half of the 4 fruits in this pic) the fruits are "disconnected" from the ...

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