0
votes
1answer
52 views

What's food mode?

I've been watching a The Crocodile Hunter episode about Komodo dragons, and Steve Irwing kept speaking about the animals being in "food mode", in which they apparently change their behavior to one of ...
1
vote
1answer
31 views

cell uptake prediction

I'm generating random molecules I would like to know if they are able to pass through the cell membrane. Are there any ways (preferably computational) to predict cellular uptake of an organic molecule ...
1
vote
1answer
228 views

Mapping a mutation to known SNP, 3' UTR, miR

I've parsed out a very large TCGA cancer ssm (single mutation file) file to give me the essential information. The ssm is in the following format: ...
3
votes
4answers
123 views

How did the activities of protocells become inheritable?

I have learned that first a protocell came to exist and its characteristics came to be passed on by genetic material. So how come genes for all the activities come to incorporate into genetic ...
6
votes
1answer
553 views

Cat purring: What are some possible underlying mechanisms behind purring and bone remodeling and formation?

http://www.scientificamerican.com/article.cfm?id=why-do-cats-purr The article above says that cats purr mostly when they're wounded or under duress. They hypothesis that cats purring leads to ...
1
vote
0answers
187 views

What mammalian cell lines are easiest to grow and transfect? [closed]

I'm working with HEK293 cells, which are very easy to transfect. Does anyone have other suggestions for cell lines with very high transfection efficiency? Ideally the chromosomes would also be diploid ...
1
vote
0answers
56 views

Reverse complement of reconstruction model for assembling reads

One way to assemble fragments produced by DNA sequencing (often called reads) is to seek for the shortest common superstring that contains all the reads of a given set of reads. One model for this ...
2
votes
2answers
2k views

Why does the sweat of children not smell like adults?

My kids sweat plenty in this hot heat, but they don't smell afterward. I on the other hand need all kinds of deodorant. Why doesn't kid sweat smell?
1
vote
1answer
35 views

Transplantation of stem cells

During the autologous transplantation of stems cells from bone marrow to produce new blood cells why all the other stem cells other than the one obtained are destroyed?
5
votes
2answers
574 views

Can proteins move outside cells?

I am trying to learn about basic cell biology, and have what is probably an extremely simple question. So this is how I understand it so far: Proteins are made from amino acids. This process is ...
4
votes
1answer
47 views

Immunity during pregnancy

I have learnt that our immune system distinguishes self from non-self by using MHC. So a mother during pregnancy should also develop antibodies against the fetus as it is also a genetically different ...
1
vote
3answers
359 views

Diffusion across cell membranes

I got this question and don't really understand the difference between the answers. Diffusion is (in cell membrane): a) passive by nature, no metabolic energy is needed b) driven process by ...
1
vote
1answer
61 views

Is Schoons Hard Shell muskmelon a hybrid?

Would Schoon's Hard Shell muskmelon be considered a hybrid as I have read it was a cross between a Bender's Surprise and a Honey Rock.
5
votes
2answers
295 views

What medical and commercial proteins are made using genetically modified animals?

Recombinant technologies in micro-organisms being used to produce commercial and medically useful proteins like insulin are fairly common. However some proteins are still produced commercially in ...
1
vote
0answers
37 views

Hippocampal regulation of hypothalamus [closed]

What is the difference between CA1-CA4 hippocampal subfields and dentate gyrus (DG) in negative regulation of hypothalamus? I`ve found only evidence that GR expression is more in CA1-2 and DG, while ...
2
votes
0answers
44 views

Endophytic Xylariaceae: diversity and taxonomy inferred from rDNA sequence analyses

"Thailand is considered as one of the areas containing a high percentage of unknown taxa of Xylariaceae (Rogers 2000). In Thailand, several studies on endophytic fungi have been documented, namely, ...
1
vote
0answers
211 views

By what mechanism does taking a shower wake up and energise? [closed]

My personal experience is that a warm or hot shower can make me feel good, alert and energised. I've heard others having similar experiences, some mentioning cold shower as a good method to wake up. ...
0
votes
1answer
392 views

TCGA data, and bioinformatics design questions for SNP/ mirna analysis

It's my first time posting to this forum but was looking for some help on the data aspects of this project. My tools of choice would be in python/R . Goal: I'm looking to create a disease specific ...
2
votes
0answers
51 views

How to use mechanical microstrainer to extract tissue proteins from human? [closed]

Background: There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
1
vote
3answers
130 views

What kind of arthropod/animal is this?

Can anyone identify this strange creature? I live in a very urban part of Montreal, Canada. It wasn't moving (seemed dead) and although the image is huge, it wasn't as big as it seems (a couple of ...
1
vote
1answer
2k views

How difficult is to renature a protein?

I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
1
vote
2answers
205 views

Has medical progress stopped human evolution? [duplicate]

We can cure many diseases nowadays, and thus the natural selection is very limited. Plus, mankind spent the whole beginning of its existence in almost the same hostile environment, and that's where he ...
1
vote
1answer
58 views

What is the disadvantage of having no anticoagulation after pulmonary embolism?

Is chronic anticoagulation always given after pulmonary embolism? What are the risks or disadvantages if anticoagulation is not given? if terminated early?
3
votes
1answer
148 views

By what mechanism is Streptococcus bovis acting as a risk factor for colorectal cancer?

Streptococcus bovis bacteremia/endocarditis is considered a risk factor for colorectal cancer. What pathophysiological mechanism may link the two together?
0
votes
1answer
659 views

Writing methods section on PCR amplication in a paper

Considering that I am writing a paper for a journal, could the following phrase be understood? or should I put the 'step-by-step' way by giving numbers? PCR Amplification and sequencing PCR ...
1
vote
1answer
56 views

What's seen when stress echocardiography shows ischaemia? Can this happen without angina?

What can be seen on the screen when stress echocardiography (a pair of heart ultrasounds before and after exercise) suggests ischaemia? Can this happen without chest pain? Illustration from ...
2
votes
3answers
1k views

Primer design for Gibson assembly

I'm trying to design a primer for Gibson assembly. My gene of interest is on a plasmid, and I want to copy that gene, and put it into a different plasmid. I am unsure how to design my primers for ...
3
votes
3answers
555 views

Why do neurones use chemical signalling at synaptic junctions?

Problem. When a neurone fires, it sends an electrical signal that jumps down the axon via the nodes of Ranvier very rapidly. At a synaptic junction, chemical brownian diffusion signalling with ...
3
votes
1answer
967 views

What is the purpose of Y-shaped adapters in Illumina sequencing?

Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library. The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary. ...
1
vote
2answers
518 views

General undergraduate zoology textbook recommendation?

I am very interested in zoology,and I want to study zoology in my spare time. Any undergraduate level zoology text book recommendation?
3
votes
3answers
1k views

Knockdown of long noncoding RNA (lncRNA) - how is it done?

I don't work at the wet lab and don't know all the details about the knockdown techniques. My question is: How lncRNA knockdown is done? For example - you have lncRNA that is functional in the ...
0
votes
1answer
100 views

Are humans more aggressive during a full moon? [closed]

Is it true that we are more agressive during the night when there is a full moon? If true, could this be a remnant from times where we needed to hunt for our food and so could see more at night when ...
5
votes
1answer
163 views

What effect do oligosaccharides, like those found in legumes, have on the composition of intestinal flora in humans and if so how so?

I know that intestinal flora metabolising oligosaccharides, like those found in legumes, is the cause for the well known fact that legumes cause flatulence, but does an oligosaccharide-rich intestine ...
6
votes
1answer
495 views

Why does it hurt more when you touch a nerve directly?

I am not a biologist nor know much about biology (so please explain in layman's terms) however I have always been curious as to why this is. What causes the difference in pain between touching an ...
2
votes
1answer
256 views

How is transport of glucose into prokaryotic cells different from transport into eukaryotic cells?

I was reading page 92 of Fundamentals of Microbiology, 4th edition, which states In facilitated diffusion, the substance (glucose, for example) to be transported combines with a plasma membrane ...
2
votes
1answer
39 views

What are ways to compare the “costs” and “gains” of food production systems?

I'm looking for methodologies to compare the costs a food production requires with the gains it delivers (not in economic terms). I'm aware of energy returned on investment. Are there other methods ...
9
votes
3answers
743 views

How are our senses dimmed during sleep?

Our senses are not as sensitive during sleep compared to wake. There is an arousal threshold during sleep that only when it is overcome will it wake a person up. My question is how is this arousal ...
7
votes
1answer
2k views

Why aren't organelles considered alive?

I'm taking a 3-week Bio I summer course, and my textbook says the cell is the smallest/basic unit of life. I asked my professor why organelles aren't a living unit, and her reply was that they aren't ...
2
votes
1answer
423 views

Why is the blood pressure in the superior vena cava during inhalation less than during the exhalation?

For further investigation into the progress of the blood pressure, we will measure a patient's blood pressure in the superior vena cava measured during inhalation and during an exhalation. His heart ...
4
votes
1answer
118 views

What are some examples of scaling laws in biology?

I've seen that metabolic rate scales logarithmically as function of mass for many animals over an extremely large span of parameters. What other scaling laws exist at the individual level?
2
votes
2answers
389 views

Why is a slow worm not considered a snake?

Slow worms are considered lizards as opposed to snakes, both are reptiles. Now I get that there are traits that distinguish them (eye lids, ears ...). But snake species themselves vary already quite a ...
6
votes
4answers
506 views

If Evolution Is In Progress, Why Fight Extinction?

Natural selection is a central tenet of evolution. However, most biologists seem determined to prevent the extinction of the species that have been selected against. Why is this? Preservation of ...
3
votes
0answers
91 views

Why do ants dig surface paths?

A few days ago I observed a corridor built and used by ants that went over a path in fields: Why do they build it? It seems it doesn't bring any considerable protection from the surrounding ...
2
votes
1answer
845 views

Why is Sanger sequencing inferior for detecting SNPs in cancer cells?

I am familiar with Sanger sequencing, but at the level of an undergraduate. A lecturer of mine tried to describe Sanger sequencing as losing the sequence information in noise when used to detect ...
0
votes
1answer
67 views

SNPs mapping into protein

Starting a new project on protein-protein interactions and SNP analysis tool development. I would like to ask how does SNPs is mapped into protein? What does mapping means?
2
votes
1answer
43 views

Storing Apples at 30F?

This article about storing apples for the winter says: You do not want the apples' temperature to fall below 30°F (-1.1°C), however, because that will make them freeze and turn to mush when ...
4
votes
1answer
471 views

Breeding laboratory mice with cancer; how does this work?

It has recently been brought to my attention that live laboratory mice with specific cancer strains can be readily purchased for medical research. For example, the Jackson Laboratory sells mice with ...
1
vote
1answer
406 views

What is solid-phase bridge amplification?

During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
5
votes
2answers
5k views

What's the difference between shotgun sequencing and clone based sequencing?

What are the main differences between shotgun sequencing and clone based sequencing?
7
votes
1answer
721 views

Does the human ear adapt to noise levels?

I have noticed lately that if I go to sleep, when I have my radio running, it is on lowest volume, I still consider it kind of loud. In the morning, when being on the edge of waking up, I hear the ...

15 30 50 per page