5
votes
3answers
188 views

How easy is it to carry out de novo sequence assembly?

Today a colleague of mine asked the following question: " Assuming I need to build from 0, a chromosome of a fish, with short reads but no other reference whatsoever [de novo assembly]: ...
3
votes
1answer
135 views

What is the most genetically simple organism except viruses?

What is the most genetically simple organism (except viruses) on this planet? By simple I mean the least number of genes.
0
votes
1answer
58 views

How do transplanted stem cells find their way to bone marrow?

Miraculously, transplanted stem cells find their way to the bone marrow missing the stem cells. How do they achieve this?
-4
votes
1answer
80 views

Basic definitions for biology terminology [closed]

I am currently writing a mathematical biology paper, however it is very hard for me to follow the existing literature due to specialized terms. I have tried googling but most explanations are very ...
4
votes
1answer
108 views

pfu Turbo DNA polymerase AD

What is the duration (Life") of pfu turbo DNA polymerase AD. I am trying o Amplification a 3.3kb fragment and the PCR schedule is 6 hours long. The manufacturers notes just specify that its activity ...
1
vote
0answers
51 views

What is the cause of singularity in phase response curve of human sleep and bright light?

I have no background in science as I am a computer programmer, but I am trying to get information on phase response curves, human sleep and exposure to bright light as I am trying to get to grips with ...
3
votes
1answer
83 views

How can this bird be identified? Can someone please identify this bird?

These photographs have been taken in Warora, India. Could someone please help identify this bird?
4
votes
1answer
66 views

What evidence is there on the medical effects of circumcision?

If this is the wrong place to post this question, then I will delete it and post it were it belongs. I have heard many conflicting things about circumcision. I have heard that it reduces the rate of ...
5
votes
1answer
159 views

What about 23andMe's SNP test gives it such bad efficacy as a diagnostic tool?

The recent news about the FDA stopping the google backed 23andMe service selling any more kits got me thinking. I understand the company may have been selling it as a medical tool prematurely, but ...
1
vote
1answer
160 views

What type of mutation causes Progeria?

I'm doing my High School biology final on Progeria, and am wondering what type of mutation causes this disease. I know that the LMNA gene codes for the "prelamin A" protein, and that protein contains ...
1
vote
1answer
57 views

Clarification of the procedures to amplify cDNA needed

The typical procedure to obtain cDNA from genomic extracted DNA is the following: RNA is extracted from desired tissue, RNA/DNA hybrid is obtained by virtue of reverse transcriptase RNA dependent DNA ...
4
votes
1answer
51 views

Finding RNA Loops

I was rooting through the literature, but there is too much work on the topic of detecting loops in RNA and the RNA helices that go with them. Can anyone point to a good review on this topic or even ...
2
votes
1answer
29 views

Difference vector target and genomic target

In Pfu turbo DNA Polymerase AD information sheet (and possibly in many other) different amplification targets are divided in Genomic DNA targets, Vector DNA targets and cDNAs. What is the difference ...
0
votes
1answer
223 views

Static in limbs

I have noticed that while sitting in the Sukhasan posture(picture below) for a long time, my feet suddenly become all stiff and i am unable to move it. What I deduce from the experience is that it is ...
20
votes
4answers
8k views

How many genes do we share with our mother?

Somewhere I have read we share more than 99% of our genes with every other other person and 98% of our genes with chimpanzees. What does this mean? Don't we share 50% of our genes with our mother and ...
4
votes
0answers
249 views

Statistical Coupling Analysis (SCA) to identify coevolved residues: use of ICA [closed]

We've begun to try out the SCA Matlab toolbox (latest version) downloaded from Dr. Rama Ranganathan's website, and, following the included tutorials, would like to apply it to our protein family. The ...
4
votes
1answer
3k views

Under what conditions does the body burn fats, proteins and carbohydrates?

Searching the internet, I have found lots of conflicting information from various fitness sites concerning when the human body burns fats, proteins and carbohydrates. My basic understanding was that ...
1
vote
0answers
21 views

mixing beer with spirits, increase alcohol content? [duplicate]

I decided to mix beer with vodka hoping it would make me more drunk due to the alcohol content but then remembered the rest ISNT alcoholic and i cant figure out if mixing beer with vodka or other ...
1
vote
3answers
357 views

What does orbital mean in orbital frontal cortex?

Orbital frontal cortex is where decisions are made. What does the word orbital there mean? I looked around in wikipedia and never find it.
3
votes
2answers
78 views

Why not use Cu(OH)2 as a dye for protein electrophoresis?

As I know, $Cu(OH)_2$ $+$ Protein $\rightarrow$ Purple. So why not use $Cu(OH)_2$ as dye in protein electrophoresis?
2
votes
1answer
60 views

Why is Thermus aquaticus and Taq Pol stable?

What is the molecular basis of the stability of the bacterium Thermus aquaticus and the enzyme Taq Pol it produces ? I have tried googling this but did not get a good answer.
1
vote
1answer
116 views

How is deviated nasal septum related to cold

I have a deviated nasal septum and I always suffer from common cold I was just wondering how a deviated nasal septum can be the cause for chronic common cold
5
votes
2answers
823 views

Isotype control antibodies in Flow Cytometry

In a Flow Cytrometry, one can add an Isotype Control Antibody to allow another antibody to bind more specific to the cells. My question is, how can the Isotype Control Antibody add specificity to the ...
0
votes
2answers
139 views

Epinephrine vs. Adrenaline

Both names are widely used, with what appears to me as a slight prevalence of “epinephrine” in scientific literature and an overwhelming prevalence of “adrenaline” in popular media. Are there any ...
5
votes
1answer
48 views

Is there any evidence telling us what the oxidation state of early earth's atmosphere was?

I ask this question in relation to the origin of life. I realise it would probably be better suited to a geology stack exchange, but it does not yet exist. The Miller-Urey experiment gives a lab ...
0
votes
1answer
114 views

Does avocado and orange have a common ancestor?

While I'm eating my orange, I felt as if I was eating avocado. It might be genetic manipulations perhaps but it made me think if they have a common evolutionary ancestor. Is there?
3
votes
2answers
1k views

The number of nipples (breasts) a species has?

Only mammals have mammaries and mammary glands. What evolutionary factors determines the number of mammaries (nipples/teats/breasts) a species has? Is it always an even number?
2
votes
1answer
382 views

Excretion of various wastes and water requirement

A common fact is that Ammonia, Urea and Uric acid are the most common excreted metabolites and their removal needs varying amount of water, highest for ammonia (hence suited only for aquatic animals) ...
2
votes
3answers
170 views

Bacterial cell lysis buffer used in proteomics procedures

What kinds of detergent-free bacterial lysis buffers exist? The proteins we're extracting will be later analyzed by LC-MS/MS, and we're looking for a lysis buffer that won't interfere with this ...
6
votes
3answers
5k views

What is the distinction between chemokines, cytokines, interferons and interleukins?

They all seem to describe molecules of similar function and many people seem to use them interchangeably. Also please include any other similar molecules if I've forgotten any in the list above.
1
vote
1answer
18 views

Cancer history on environment-cancer relationship

I am interested to know how environment-cancer relationship knowledge has developed. I know that all began when Muller first proved that mutation could be induced via ionizing radiation, X rays in the ...
3
votes
1answer
78 views

Chemoautotrophy in large organisms?

The major nutritional mechanisms include chemoautotrophy, heterotrophy (including parasitism and saprotrophy) and photoautotrophy. All of these modes of nutrition developed first in prokaryotic ...
4
votes
1answer
73 views

How predictably will RNA polymerase not translate repetitive sequences?

I am working on an (expensive) synthetic construct, which happens to have many "repetitive" sequences within it that are integral to its function. Primarily, the two sequences that are worrying me ...
2
votes
1answer
73 views

Is there a limit to acceptable brightness for plants?

Can overly bright artificial light harm plants? Is it possible to achieve a level of brightness so high that it does more harm than good to plants? If sunlight can be filtered of infrared light ...
0
votes
4answers
287 views

Punnett Square Help

In fruit flies, red eyes are dominant over white eyes. Show a cross between two white-eye fruit flies. My question is... How do I know if the white-eye fruit flies are homozygous or heterozygous?
2
votes
0answers
72 views

Organisms with the most extreme sensory capabilities? (sight, smell, sound) [closed]

Background It is quite mind boggling to imagine what it would be like to be missing sight, sound, touch, smell, taste etc. Question Which organism holds the record for most extreme sensory ...
4
votes
1answer
323 views

How much does the Hayflick limit/telomere length vary across taxa and within humans?

Since they seem to be quite connected, I'm curious if anyone knows of research comparing the Hayflick limit (and presumably by extension telomere length) between different taxa. I've heard the ...
4
votes
0answers
175 views

Genetic expression in interspecific hybrids

Referring to interspecific hybrids, I have the following two questions:- Quoting from wikipedia:- The offspring of an interspecific cross are very often sterile; thus, hybrid sterility ...
2
votes
2answers
78 views

How does promoter sequence affect initiation?

I don't know if this might have been highlighted in recent research, but a textbook I have states that "the exact way in which promoter sequence affects [transcription] initiation is unclear" I'm ...
1
vote
1answer
1k views

Cats, Dogs and Bears - how are they related?

What is the ancestral relationship between cats, dogs and bears? I presume they are more closely related to one another than other paraphyletic clades of mammal. Which came first? What did their ...
1
vote
1answer
687 views

Does mitosis include cytokinesis?

I found that some books include cytokinesis within mitosis, but some other books I have referred to include only the dividing of the nucleus as mitosis and do not include cytokinesis. Can anyone help ...
3
votes
2answers
2k views

Cell life: division for immortality or reproduction with aging

Are the two cells that are derived from one cell, ‘twin sisters’ or a ‘mother and a daughter’? In other words, can a cell really be divided to live an "immortal" life or is cell reproduction the ...
1
vote
0answers
31 views

RT-qPCR of selected differential expressed genes: which dilutions in calibration curve?

I've seen in MIQE guidelines that calibration curves a needed when performing RT-qPCR. I'll use genomic DNA to perform these calibration curves. My question is that I don't know the range of ...
2
votes
1answer
88 views

How many plants are needed to survive in an airtight chamber?

How large a garden do you need if you are to survive on them producing enough oxygen in a closed chamber? And which are the most effective plants?
1
vote
2answers
174 views

Effect of zero selection (pressure) on the population health

Evolution naturally produces better features: stronger muscles, teeth and minds. Killing the weakest, evolution wipes defective genes out of populations. The mutations are necessary for advance. ...
1
vote
1answer
44 views

Removing Sugars Bound to a Protein

I recently purchased some Maltose Binding Protein and as the name suggests this protein binds to maltose. The problem I have is the protein arrive with maltose bound. I know this through native mass ...
3
votes
1answer
276 views

Is there a maximum pain threshold?

Is there a maximum threshold of pain a Human can experience, beyond which point there is no noticeable difference? I ask this question in part to better understand the definition of pain and its ...
1
vote
0answers
16 views

Are there any differences between qRT-PCR and GEO (microarray data from NCBI) regarding dealing with internal control genes?

As we know, when we do qRT-PCR, the two most commonly used methods are absolute quantification and relative quantification, both the methods are involved in dealing with normalization of house-keeping ...
2
votes
1answer
236 views

Is tongue rolling genetic?

Is the ability to roll your tongue or not truly inherited or genetic? What I have gathered from school has been contradicting with evidence I found elsewhere.
0
votes
1answer
89 views

How do birds learn their tunes in isolation from their own species?

I wonder what a bird would sing if it didn't have its parents around (or any other birds for that matter) to learn its chirping sounds from. I'm interested in how a bird would sing... in complete ...

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