6
votes
1answer
146 views

If life is discovered on another planet, will it likely be classified using the current domain/kingdom/phylum system?

This may be a stupid question showing my lack of understanding of taxonomy, but I was wondering if the current classification system we use contains broad enough categories to include new life forms, ...
5
votes
1answer
111 views

Significance of basal lamina for outer layers of epithelium

In stratified (not pseudostratified) epithelia such as the epidermis, what purpose does the basal lamina serve for the outer layer cells which do not even stand in contact with it? Also, how do these ...
5
votes
2answers
422 views

What metabolic activities are performed by a developing human fetus's liver?

I understand that organ function varies with the stages of development. Does a fetal liver EVER perform lipogenesis, gluconeogenesis, make bile, etc? Or does it only begin performing these actions ...
3
votes
1answer
141 views

How might gene clusters like the lac operon evolve?

The obvious answer for a gene cluster is gene duplication and mutation of one or both genes. But the genes in the lac operon seem not be so functionally/structurally similar (as compared to the Hox ...
6
votes
2answers
683 views

How long can I store autoclaved disposables and reagents?

I want to stock up on autoclaved disposables, such as Eppendorf tubes, pasteur pipettes etc., and some buffers. If I don't open the container after autoclaving, how long can I store autoclaved ...
1
vote
1answer
235 views

What is the brightest color a light source that only emits blue frequency light can achieve?

Let's say there is a luminous light bulb that only emits blue light, and you (or a camera if you prefer) look at it from a close distance. Will the maximum brightness perceived not have any "white" to ...
0
votes
1answer
266 views

Least painful way to die

There is a lot of talk about inhumane treatment of animals and how we don't kill them in the proper way. I wonder how much research is done on this subject. What is the overall least painful way for ...
0
votes
1answer
590 views

DNA gel extraction: chemical contaminants

I am performing a gel extraction to purify DNA after a double digest with EcoRI and BamHI. After the gel extraction I need to complete a ligation step before bacterial transformation. The problem I ...
2
votes
0answers
46 views

Onset of Autophagy

Fasting and Intermittent Fasting (IF) have been proven to start autophagy (cellular self-digestion). How long does the average man and woman have to wait for autophagy to begin? Does diet prior to ...
1
vote
1answer
40 views

What does “Psychomodulatory” mean in the context of potentially psychoactive drugs?

What does "Psychomodulatory" mean in the context of potentially psychoactive drugs? That is, in this journal article http://www.sciencedirect.com/science/article/pii/S0028390812001931 in the ...
0
votes
1answer
462 views

What's the difference between life expectancy of cigarette smoker and general population?

Some say that smoking cigarettes will shorten lifespan. By how many years is the lifespan of a typical smoker shortened? What are the common cause(s) of death among smokers? Are there any known ...
1
vote
1answer
50 views

How are geneticists able to isolate gene functions?

As an example, this Scientific American article describes a known area in the dog genome that metabolizes carbohydrates. How is it that researchers are able to determine specific functions such as ...
3
votes
1answer
2k views

What factors are known to affect evolution?

My understanding is that the possible mechanisms of evolution are: Environmental changes Cultural/mating preferences Population Immigration Genetic Mutation Am I missing anything? I've heard that ...
1
vote
0answers
76 views

Can mammals from different species reproduce?

I'm curious on how genetically different can two animals be in order to reproduce. Could they belong to different species? One example is the mule which is the offspring of a donkey and a horse, in ...
8
votes
1answer
860 views

Under what circumstances and why is terrific broth preferred to LB broth for E.coli growth?

Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
4
votes
1answer
1k views

What is immunopanning (vs. immunoprecipitation and FACS)?

I had never heard the term before today. From what I can tell, it's using antibodies to purify a cell population of interest. I would appreciate more details, especially in how it differs from ...
0
votes
2answers
130 views

Why do some people find vegetables so repellent when evolutionarily they should find them an attractive and thus tasty food?

Why do certain people (especially kids) find vegetables so "repellent" when evolutionarily they should find them an attractive and thus tasty food? I ask this question because if Darwin's theory of ...
2
votes
0answers
67 views

Is there a Pharmacology Textbook that Satisfies the Conditions listed in the Body of this Question?

The conditions are: As extensive and explanatory as Goodman and Gilman's The Pharmacological Basis of Therapeutics, Twelfth Edition With additional extensive and thoroughly explanatory information ...
2
votes
1answer
109 views

Expanding the SETI initiative to seek intelligent data within DNA sequences?

I've recently read a couple articles dealing with long term data storage, and DNA was suggested as one of the prime candidates for long term storage of digital data additionally, there is this article ...
1
vote
1answer
103 views

What is background binding?

I'm reading a journal article about mitochondrial protein import and it mentions that one of the mutant proteins tested showed background binding with another protein. What the heck is background ...
3
votes
1answer
309 views

Can Naegleria fowleri enter through the eyes ( example rinsing/splashing eyes with water)

I understand that it primarily enters the body through the nose by by "feeding on the olfactory neurons in the nose" as answered here in a different question. Would the nerve cells in the eyes present ...
3
votes
0answers
25 views

A program for cell motility assessment with a batch process function?

Cell motility assessment is a branch of experimental biology or medical science. One example could be an assessment of treatment effects on sperm motility of an animal. The standard procedure involves ...
1
vote
1answer
66 views

Is stereo olfactory ability restricted to humans?

Most animals I see around seem to have two nostrils - humans, snakes, birds, fish .. and so on. From reading online I see that 2 nostrils provide a stereo olfactory effect. This stereo effect is ...
10
votes
2answers
170 views

What makes E. coli yellowish?

LB, TB media, and yeast extract are also yellow but at the heart of the question. What chemical(s) make E. coli yellow?
4
votes
1answer
128 views

What limits the size of fish compared to marine mammals?

The largest extant fish species is the whale shark (Rhincodon typus). With a length of 13 meters and a mass of about 20-30 metric tons, the whale shark is relatively small compared to the largest ...
0
votes
0answers
50 views

what's an ethics-friendly way to measure the protocol of electric shocking mice?

We have to measure the protocol of electric shocking mice. The mice are head-fixed, we have to guarantee that the electric current is significant to make the mice learn, but not too big to make the ...
2
votes
3answers
1k views

Improving Gel Extraction yields

How can I improve my Gel Extraction yields. We use the standard protocol from Qiagen, gel extraction, dissolve in QG buffer at 42C and purify via anion exchange columns. However, with 500 ng we ...
4
votes
1answer
187 views

What is a simple protocol for staining cells in suspension?

I am an engineering student studying how electric fields affect cells, specifically the phenomena of electroporation in living cells. I know that electroporation is widely used for introducing genes ...
2
votes
1answer
178 views

One flybase gene number (FBgn), many Affymetrix Id's

I am trying to convert a set of Affymetrix ID's, like this one 143053_at_3745, to Flybase Gene Numbers (FBgn) like this one FBgn0000015. I have downloaded the Flybase file required to do so (as ...
6
votes
1answer
294 views

Why human cannot stand still in one place?

When a human stands in one place, he cannot stand still but we can see the body slightly swings. Is this due to the Earth rotation or it has something with biology and muscles relaxations? I have ...
2
votes
1answer
71 views

What causes tree lines?

There is an area of where there is a distinct line of only trees on one side and only shrubs on the other (right next to each other). What could cause this? My attempt: I said there could be a ...
3
votes
1answer
143 views

What kind of caterpillar is this?

I've made a photo using the reversed 58 mm lens and my cropped DSLR: Could you please help? I really don't know where this came from. I found it in Russia.
1
vote
0answers
146 views

Are there nutritional benefits to drinking diluted urine?

I know that urine drinking is a widespread practice (with a long history). The main reason that people give today involves there being nutrients in urine. If there are nutrients to be gained, why is ...
4
votes
1answer
58 views

Do kidneys have the enzyme 3-ketoacyl-CoA transferase?

I am thinking if kidneys can use ketone bodies as fuel. Ketone bodies are water-soluble so no problem because of solubility. The existence of the enzyme determines the answer. Can kidneys use ketone ...
1
vote
1answer
73 views

Is there an optimal illumination level for human habitats/workplaces?

I know of melanopsin, a photo pigment in mammal eye that helps regulate circadian rhythms. It responds most strongly to blue spectrum light. There have been experiments that demonstrated that ...
2
votes
1answer
408 views

What controls the breast size?

I am not a biology student, just a curious person. To be specific, I'm talking about humans and specifically about females. I know that it might apply to other mammals. What controls the size of ...
1
vote
0answers
40 views

Why is important to get saturated fatty acids from diet?

I am thinking why our body must get saturated fatty acids from diet. We can make them, since they are not essential fatty acids. The body can make all fatty acids except linoleic acid and ...
3
votes
2answers
97 views

Do dolphins really sleep?

I have always wondered, if dolphins sleep with one eye opened, do they really sleep at all? According to this article dolphins indeed shut down their brains. I already googled it. No help from ...
0
votes
3answers
211 views

Can this theory on the evolution of human appearance be flawed?

This is what a friend of mine said: When there's human offspring, it will look a rough 'medium' between the two parents, with it sharing features from the two. well if the offspring of one set ...
0
votes
1answer
44 views

Complexity in creating transgenic animals (e.g., mice)

Many papers I have seen describing transgenic rodent models (and presumably applicable to other model organisms) involve the knock-in, or modification to, a single gene, possibly two genes. With ...
3
votes
2answers
82 views

Is there a way to measure the amount of bytes that are possible to encode in a DNA molecule?

When I saw a DNA molecule for the first time, it kinda reminded me of a hard drive. It consists of slots and there are some possible combinations for each slot; in the hard drive these possible ...
0
votes
1answer
97 views

RACE pcr product

I'm a molecular bio novice, and I'm trying to understand the differences between regular PCR and RACE PCR. If it wanted to sequence my PCR product that might be hundreds, if not thousands of bps ...
2
votes
1answer
115 views

What's the difference between a free chromosome fragment and an extrachromosomal array?

This is reference to a review on C. elegans mosaic analysis by Yochem and Herman, in which the authors make a distinction between free chromosome fragments and extrachromosomal arrays. For the ...
4
votes
0answers
55 views

Does preservation in ethanol alter leaf litter mass?

I have benthic samples that were collected with an Ekman dredge from some small ponds. The samples contain quite a bit of coarse particulate organic matter (CPOM, basically dead leaves). I would ...
8
votes
1answer
376 views

Can Bioluminescence drive photosynthesis?

I'm trying to construct an anaerobic kitchen-waste digester at home. The major output from the digester is methane - with a significant component of carbon dioxide. To scrub/reduce the CO2 I was ...
4
votes
2answers
3k views

Why is that all the animals can swim in water without learning to swim but humans cannot?

I had a question since I was a child. I was always curious that all the animals can swim in water. They don't need any training or to learn swimming. But humans need to learn to swim. Why ? I am a ...
-3
votes
2answers
104 views

Specific enzyme for C. elegans [closed]

We are looking for an enzyme that appears in C. elegans but isn't common for: human, Drosophila or mouse. We need it for a little research project, thanks for your help!
2
votes
1answer
61 views

Are (baseline) high blood levels of melatonin associated with either circadian rhythm or mood disorders?

(Exogenous) melatonin is administered at night for treatment of circadian rhythm disorders, but melatonin taken during the day is linked with depression. I would like to know if high blood levels of ...
3
votes
0answers
78 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
7
votes
3answers
7k views

Why do I smell flowers after sneezing?

Often when I sneeze, I smell flowers for a few seconds afterwards. I've done some searching on the internet, and apparently it's something that is at least not entirely uncommon, although the exact ...

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