1
vote
1answer
35 views

What are the movement mechanisms of thrombus?

I am thinking how thrombus (veins, arteries and heart) can move. Secondary mechanisms come to my mind only: some enzyme which lyses it, probably adhesion mechanism. Blood circulation (flow rate) ...
3
votes
1answer
243 views

Benefits of CLARITY?

What are the benefits of CLARITY over this technique that was published more than a year earlier? Of course the second technique needs a fancier microscope that is likely more expensive and requires ...
0
votes
2answers
137 views

PAH gene mutation

Analysis of the DNA of the Phenylalanine hydroxylase (PAH) gene in a patient with phenylketonuria revealed a mutation in the protein coding region whose predicted effect would be to replace the amino ...
1
vote
1answer
85 views

Diffusion of FAD+

Why is NAD+ free to diffuse within the mitochondrion whereas FAD+ is not ? What biochemical properties cause this difference ?
0
votes
2answers
81 views

Why is succinate dehydrogenase attached to the inner mitochondrial membrane?

Succinate dehydrogenase is attached to the inner mitochondrial membrane.All the other enzymes of the Krebs cycle are located in the matrix of mitochondria. What is the biochemical reason behind ...
3
votes
1answer
88 views

Comparing SIFT and PolyPhen to other methods

I'm looking for a database of nsSNP (non synonymous single nucleotide polymorphisms) and their effects. As I understand, the common methods to predict this are SIFT and PolyPhen, and I want to compare ...
1
vote
1answer
474 views

basic programming and bioinformatics [closed]

As a molecular biology graduate student I have decided to learn some basic programming and bioinformatics since everybody says that it is crucial. For example, what would you learn if you need to work ...
1
vote
1answer
55 views

RNase and EtOH issue

I work with RNA and I have some RNase issues. For example I am using RNeasy mini kit and at one step I use 100% EtOH. It does not say it is RNase free or not. Does absolute EtOH inhibits or removes ...
2
votes
2answers
124 views

Is there any evidence of increased life expectancy for animal species?

Life expectancy for human has significantly increased during the last century or so. We all know that there are many reasons that are not linked with "evolution", but I am wondering if such change in ...
0
votes
1answer
94 views

calculate the number of heterozygotes [closed]

The ability to taste PTC is due to a single dominant allele "T". You sampled 215 evolution students, and determined that 150 could detect the bitter taste of PTC and 65 could not. Assuming this trait ...
1
vote
0answers
214 views

Microtiter Dish Biofilm Formation Assay- Pseudomonas and Crystal Violet

If Pseudomonas is a gram negative bacteria, it does not retain crystal violet but why is it that so many people are using crystal violet staining in theri Microtiter Dish Biofilm Formation Assay?
1
vote
1answer
39 views

Reverse tomography to create 3D scalpel

Isn't it possible to reverse tomography methods to create means to deliver heat or radiation precisely in 3D? This would be "3D scalpel" i.e. device which would be able to eradicate tumor cells ...
0
votes
1answer
26 views

How many systemic reactions in inflammation?

I have this sentence in my notes Inflammation consists of two local reactions and one systemic reactions. which is difficult for me to accept. Two local reactions are vascular and cellular. I ...
2
votes
2answers
79 views

Clarification on Hatch and slack pathway

The following is a minor clarification that I want to make, since it is rarely addressed directly in most of the texts I have gone through. In C₄ pathway, the chloroplasts are dimorphic, that is, ...
1
vote
2answers
55 views

Multicellular organisms as DNA banks

Animal -> bacterial gene transfer seems easier than bacteria -> animal (rotifers are an interesting exception); there are bacteria live in close contact to us but our germ-line cells are protected. ...
0
votes
1answer
40 views

Why do doctors do chemo or transplantation for cancers that don't respond well to these treatments? [closed]

From the UpToDate article on multiple myeloma: In most people, chemotherapy partially controls multiple myeloma; rarely, chemotherapy leads to complete remission. Also: Transplantation, ...
3
votes
2answers
98 views

Why do humans circulate monosaccharides instead of disaccharides as in plants?

Plants transport food mostly in the form of disaccharides like sucrose but humans transport them in the form of a monosaccharide - glucose. What is the reason behind this ?
0
votes
1answer
228 views

What is the benefit of using Gonoline-Euroline in combination? [closed]

I was heard that this combination first Gonoline and other culture another culture on Euroline is good one. What is the benefit of using Gonoline-Euroline in combination?
1
vote
1answer
35 views

What are the best detection medias for cholera?

I heard this fact that you can use some [hypertriade] for vibrio cholera diagnosis which has compontents sucrose (yellow) mannose (yellow) arabinose (do not ferment; stay dark pink) I did not find ...
0
votes
2answers
93 views

What is the right spelling for this agar? [closed]

I could not spell the agar [gonoline-uroline] which I heard yesterday. My spelling is so wrong that I could not find it in Google. What is the right spelling for this agar?
6
votes
3answers
384 views

Does a neuron ever generate an action potential without stimuli?

Most accounts I read involving action potentials and synapses and the like tend to focus mostly on the action potential as a mere automatic reaction to another similar event happening upstream. From ...
0
votes
3answers
894 views

Why do we exhale after we hold our breath?

I tested this out with my friends, and I find that after they hold their breath and can't hold it anymore, they exhale air, instead of inhaling air. Interestingly, they all try to inhale in as much ...
0
votes
1answer
53 views

Shigella's O antigen (PAI synthesised) and passage through host defences

The O antigen is synthesised by Pathology islands (PAI). O antigen may be a factor why Shigella survives the passage through host defences. I am interested in which step of pathogenesis. There are ...
2
votes
1answer
58 views

GULO only for mammals?

I am not a biology student, but just want to know if GULO gene are present only in mammals or all species possess it ? And is GULO gene active in human fetus?
0
votes
0answers
37 views

Can we define biological age? [duplicate]

How can we define ageing from a biological standpoint without making an appeal to chronological age? Related, but less important, are there any metrics we can use to determine someone's biological ...
6
votes
1answer
122 views

Hill-Roberston effects and effective population size

From this article, first page, middle of the second column: Even if harmful alleles do not become fixed, they can still reduce the efficacy of selection on neighbouring loci through a process ...
1
vote
2answers
81 views

Can viruses be used to extract oil from algae?

I read that one hard step in algae oil extraction is to rupture (or fracture) the cell walls to get the contents inside. Some people use chemical methods; some use sonication or mechanical forces. I ...
2
votes
1answer
2k views

Isopropanol precipitation of DNA - duration and magnitude of cold storage

DNA prep protocols often include a final precipitation step with alcohol, often isopropanol, where the DNA must be kept in the alcohol, at a low temperature such as -20C or -70C, often overnight. ...
3
votes
1answer
226 views

Meiosis vs. Mitosis

Meiosis is a type of cell division that reduces the chromosome number from 2n to n to make gametes viable for reproduction in humans. I know that during meiosis, there is independent assortment and ...
2
votes
1answer
116 views

Does recombination increase the additive genetic variance for fitness?

On this article, first column, eighth line of the introduction: By bringing together favourable alleles from different chromosomes, sex and recombination increase the additive genetic variance for ...
1
vote
1answer
37 views

What indices can we use to describe fitness landscapes?

We usually talk of smooth or rugged fitness landscape. Are there any (standard) indices to measure the "structure" of fitness landscapes? For example, one might consider the mean epistatic ...
4
votes
1answer
3k views

Rate of cell division in humans

On average, how many cells divide each day in a human being? How long does a cell wait before dividing itself ? I have tried to look on the internet but surprisingly the answer is difficult to ...
2
votes
2answers
186 views

Primer design and BLAST E value stringency

When searching for misprimings I have been told that an e value that is higher than 0.01 is ok and will produce no significant amounts of mispriming. Yet I searched some and it would seem that the e ...
0
votes
1answer
353 views

Difference between Cary-Blair and Amies transport media for Staphylococcus and Streptococcus?

I normally use Amies medium, but I today heard that Cary-Blair is also possible. When should you use Cary-Blair medium?
2
votes
2answers
2k views

Why there is a very high drop in pressure from arteries to arterioles?

Laplace law explains why the pressure is the same in aorta and arteries (100). I am thinking why there is so big drop in pressure when going from arteries to arterioles (40-60). Why there is a great ...
1
vote
1answer
62 views

Genome Assembly Using Reads

I'm taking an online bioinformatics class and I am stuck on a problem. The resources I have found don't help that much. Here is the problem: "Assemble the error-free reads from a circular genome on ...
0
votes
2answers
167 views

Systemic sensory confusion?

Sometimes when a person gets touched on one part of the body they feel it else where and may think it came from a different part of the body. What causes this? Other examples are similar to why you ...
1
vote
1answer
35 views

Difference between Fulminant and Acute Meningococcemias

Fulminant seems to mead rapid. There is however some articles with both phrases: "fulminant meningococcecemia" (about 5000 Google matches) and "acute meningococcecemia" (about 3000 Google matches). I ...
2
votes
1answer
42 views

What's the best way to measure/calculate the size of a light beam at the sample of a microscope?

I am using a microscope with an LED derived light through the epi-fluorescent port of a microscope. I know that the "field of view" for a given objective is equal to the field number/magnification ...
4
votes
2answers
296 views

How does the brain know where a signal came from? What is the addressing system

I am an electronic engineer so I am thinking about this from an electronics outlook. How does the addressing system work, As I see it, the nervous system is small parallel branches attached to larger ...
0
votes
1answer
21 views

Is Rheumatic fever more Chronic than acute?

It follows from the complication of S.pyogenes' pharyngitis. I am thinking how the inflammatory response behaves: acute or chronic or something between. I think chronic disease is better description ...
0
votes
1answer
91 views

M-type potassium channels by muscarinic receptors

How does the closure of M-type potassium channels by muscarinic receptors result in increased excitation of the target neuron?
0
votes
1answer
202 views

Pathway mediates nitroglycerin-induced relief from angina pectoris [closed]

what kind of Pathway mediates nitroglycerin-induced relief from angina pectoris,please provide some idea...
2
votes
0answers
124 views

Best way to automatically link Gene Entrez ID with Gene Symbol in TCGA

I am trying to figure out how to link Gene Entrez ID with Gene Symbol, for TCGA dataset. So far, I have found this ftp directory with Gene info updated daily. But, for Entrez ID 728661, I have found ...
0
votes
3answers
106 views

Phylogenetic tree

I want to find the conventional phylogenetic tree of human, mouse, C elegans and drosophila, without all the other organisms. Do you know where can I get it? thanks, Noga
0
votes
1answer
29 views

Is endoderm visible in the germ layer?

This picture is my drawing about germ layer - not embryonic folding as I wrote initially. Where exactly is the endoderm here in the picture? The known things Ectoderm Neural tube Notochord ...
4
votes
1answer
95 views

Does adding milk to cereal make it harder for our body to absorb the vitamins and minerals from the cereal?

I add about 3/4 cups of Blue Diamond almond milk (unsweetened) every morning to a single serving of Cheerios. The milk contains filtered water, and I read that drinking water with food at the same ...
-1
votes
1answer
77 views

Why do mammals in the wild retain the “trait” of menstruating with blood?

This question intrigued me a lot since if animals in the wild did have menstruation with blood flow, wouldn't all that blood be attracting every other predator in the vicinity?
2
votes
1answer
147 views

Solubility of Forskolin in ethanol

I am interested in using forskolin in cell culture medium. Does anyone know how to make solution of 10 microM forskolin in 5% ethanol or less. I would like to avoid using DMSO as a solvent. Thank you. ...
0
votes
1answer
59 views

How do neurons send electric pulses even though they are surrounded by liquid which is mostly water?

I just thought about the fact that people move through electrical pulses traveling through neurons in the brain and muscles through the body. Here is what i know: the body is full of 97 or more, ...

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