8
votes
3answers
518 views

Pipetting damage on cells

I'm curious how much damage is potentially inflicted by shear stress by pipetting. I know that with syringes for stem cell injection cause a lot of damage. However, to what extend does this happen ...
1
vote
1answer
39 views

Modelling how much cadmium a cell will be able to chelate with phytochelatins

I am involved in a synthetic biology project where I have to do modelling with ODEs. The biological process I want to model is the chelation of Cd2+ with phytochelatins, to know how much can be ...
0
votes
0answers
6 views

Pharmacology: Drug Administration

Why is the enteral route considered to be the safest way to administer a drug into the body?
0
votes
1answer
27 views

Why are human children more likely to get lice (nits) than adults?

By my own experience, lice seems to be prevalent in the hair of human children, but not in adults. My question is Why are human children more likely to get lice than adults? Assumptions: Can be ...
3
votes
1answer
24 views

Is it essential to add cholesterol to agar or liquid culture for C. elegans?

In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious ...
3
votes
3answers
49 views

Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
3
votes
1answer
20 views

Does isolation have any effect upon mouse behavior?

I work with a lot of mice. I don't do any behavioral analysis, I just inject DNA or RNA and do imaging assays. However, I have noticed some effect of isolation on mouse behavior. Normally, the mice ...
6
votes
5answers
814 views

Why do most mammals have long snouts?

It seems like most mammals, e.g. dogs, have long snouts. My pet dog's snout would seem to me like an evolutionary disadvantage, since her canine teeth are way out at the end of her jaw, which acts as ...
10
votes
4answers
676 views

Why is it sometimes difficult to resuspend E. coli in P1?

It's a curiosity question. When I'm doing minipreps after pelleting the bacteria sometimes it's very easy to resuspend them in P1 (Qiagen kit), but sometimes they form a rubbery clump that is very ...
20
votes
1answer
10k views

How does laughing gas (N2O) work?

Laughing gas (N2O), well, makes people laugh. How does just a gas make us do that, there has to be some hormones at work... So, I wanted to know how this works? What is the mechanism?
2
votes
2answers
33 views

What is a membrane potential?

I know this may be silly, but I am confused to what a membrane potential actually is. I understand that at resting membrane potential is -70- -80 mV. But what does that exactly mean and how does this ...
-1
votes
2answers
39 views

Do non-stick Teflon coated frying pans cause cancer? [on hold]

Is it true that using Teflon-coated pans contain carcinogens, and if so, can they be consumed through the food cooked in them? E.g. The deadly toxins from non-stick frying pans
2
votes
0answers
24 views

Genome-wide methylation analysis: sources of technical error?

We are doing a genome-wide analysis of methylation via bisulfite sequencing for an insect species. Previous experimental techniques have suggested the presence of methylation in this organism, ...
0
votes
1answer
29 views

Spermatogenesis; maternal homologs

What is the probability that, in an organism with a haploid number of 10, a sperm will be formed that contains all 10 chromosomes whose centromeres were derived from maternal homologs? I have ...
3
votes
2answers
2k views

How does extracellular potassium ion concentration and calcium ion concentration affect the excitability of a cell?

When extracellular $K^+$ concentration increase by a certain amount, excitability of cells is higher because the resting potential shifts toward a higher equilibrium potential of $K^+$, therefore ...
3
votes
1answer
22 views

What is the best current understanding of how yeast transformation works?

I would like to get myself up to speed with what is currently known to science about yeast transformation. Specifically, transformation of plasmids and linear DNA fragments. I am particularly ...
2
votes
2answers
25 views

Premise of life cycle synchronization between predator and prey

While reading about the predator satiation hypothesis of the periodical cicadas' 13/17 year life cycles, I started wondering about its premise. By the way, I understand the math behind the prime ...
1
vote
0answers
17 views

Evolution of sexually concordant genetic variation on the X-chromosome

It was theorised in 1984 that sexually antagonistic genetic (SA) variation should more easily evolve on the X-chromosome. This is partly because female beneficial/male deleterious mutations face less ...
1
vote
1answer
149 views

Change of phase from juvenile to adult in plants

I tried googling this but couldn't get much information on this. Most of the articles were about induction of flowering. I am more interested in knowing what factors influence the conversion of ...
1
vote
0answers
22 views

Pharmacokinetics: why do certain drugs follow zero-order kinetics?

I understand that alcohol and phenytoin are two examples of drugs that follow zero-order kinetics. Why do these two particular drugs follow zero-order kinetics as opposed to first-order kinetics?
0
votes
1answer
32 views

Are there other methods besides surgery and hormonal treatments to help undescended testicles? [on hold]

I've been doing some reading on undescended testicles and common treatments. It looks like the most common treatment for a baby is to give it time to see if the testicles drop on their own. If not, ...
7
votes
2answers
4k views

Why do dogs try to cover their poop?

It may seem obvious to us humans to hide our excreta, but this is not quite normal behavior for animals. Interestingly, dogs have the habit to pee all over the place. It's know that they do so to ...
4
votes
1answer
149 views

Microsatellite shifts (peak calling) GeneMapper! Thesis help!

I'm a masters student attempting to conduct a parentage analysis on a population of fish for my thesis. My advisor and post-docs haven't been very helpful, so I need some help! I have dinucleotide ...
1
vote
2answers
69 views

Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
3
votes
2answers
46 views

Does osmosis take place in prokaryotic cells?

As far as I know, osmosis occurs in Eukaryotic cells, and I'm wondering if it could take place in prokaryotic cells too.
4
votes
1answer
66 views

What is this creature?

Since it has six legs, I believe it's some sort of insect, but I could be wrong. I spotted it in my Central Texas backyard (Austin area). It was very slow and not at all skittish. For size ...
0
votes
0answers
11 views

How are isopotential maps computed from EEG channel data

I have some EEG recordings of $1$ minutes, with $500$ samples per seconds and $63$ channels. For each recordings, this effectively yields a $30,000\times 63$ matrix $M$. How do I extract isopotential ...
4
votes
0answers
33 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
6
votes
1answer
53 views

Are recessive, deleterious alleles less common on the X chromosome than the autosomes in humans?

As there is a potential for them to be more readily purged in hemizygous males (and in cell lineages in females with the deleterious-allele-bearing chromosome activated), I would expect the frequency ...
7
votes
3answers
16k views

What is the difference between an antibiotic and an antibacterial?

Concerning medicine, what are the differences between antibiotics and antibacterials?
8
votes
1answer
339 views

How does yeast dissolve in sugar?

There's this fascinating "kitchen experiment". Take a "brick" of baker's yeast (not dry yeast), and a couple teaspoons of sugar, put them together in a cup and stir together. There's no doubt both are ...
0
votes
0answers
10 views

Does the Y chromosome carry structural information, or only switches + traceable ID?

The Y chormosome almost doesn't crossover with the X chromosome, and so the evolution of anything it encodes must be much slower, because by the Hill–Robertson effect beneficial mutations must occur ...
5
votes
2answers
4k views

Do other male mammals except for humans have nipples?

When I grew rabbits, I had a pair, one male and one female. And while the female's nipples were quite prominent, especially after giving birth, I don't remember the male having any nipples at all. Do ...
6
votes
1answer
361 views

Do birds have navels?

This picture shows the development inside a bird egg: This shows the connection of the embryo to the yolk sac. Does this mean birds have navels? And if so, what happens to the umbilical cord once ...
0
votes
1answer
41 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
0
votes
1answer
47 views

Can a guy be turned into a girl near perfectly? [on hold]

I have always been told that during gender realignment there is some damage to the body which is why even if you had all the money in the world you can't keep changing between male and female. ...
10
votes
2answers
25k views

How many human cells are there in our body, on average?

How many human cells are there in our body, on average? Wikipedia says 1013: Bacterial cells are much smaller than human cells, and there are at least ten times as many bacteria as human cells in ...
2
votes
2answers
495 views

What happens if a person has the right side of the body totally removed (including the brain)? [on hold]

I guess humans could live without just the right lobe of the liver or the right brain. Could a person without the whole right side of body survive? The left brain controls the right side of the ...
8
votes
1answer
74 views

Dogs bark at familiar person in Santa costume. Why? Don't dogs trust smell over vision?

Last night 10 humans and 2 dogs spent Christmas together. At some point, one human dissappears for a few minutes and comes back wearing a full Santa outfit. Upon respawning, the dogs start barking ...
1
vote
1answer
13 views

can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?

Dear fellow biochemists, I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore ...
3
votes
2answers
51 views

In practice, do fishes die from old age?

I was watching sea birds eating fishes and wondered if fishes actually died from old age, or if they are all eaten before this can happen? I reckon some big predator fishes must be able to live long ...
5
votes
0answers
47 views

When did CRISPR/Cas9 evolve and what is the likelihood that a superior system for live cell genome editing has already evolved on earth since then?

I've read that CRISPR/Cas9 is currently being implemented and tested for its ability to edit genomes in live cells, and that it is supplanting other genome editing tools in labs, such as TALENs and ...
-1
votes
0answers
43 views

Is there any evidence that soft tissue can be preserved for 65m+ years and if not what hypotheses exist to explain it?

I read an article on howstuffworks.com and they mentioned how we have found soft tissue in dinosaurs. According to the article even Mummies and Mammoths have no soft tissue left. Is there any evidence ...
0
votes
0answers
15 views

Is there a natural defense against an increase in E.Coli level in natural water systems?

I was recently on an Oceanology coliform collection vessel in the Long Island Sound and I found out that human waste contains E.Coli and when not properly treated the bacteria can run off or ...
3
votes
2answers
314 views

19th century Latin species description

While reading a 19th century text of new species description, I'm confused by some Latin phrases. The case here is: Corp. long. 8 poll., caud. 9 poll. I guess 'corp.' and 'long.' are ...
2
votes
1answer
43 views

NMR spectroscopy detection methods for proteins

While studying, I've stumbled upon a table comparing the sensitivities of different detection methods in NMR spectroscopy: "Direct", "DEPT" and "Inverse", where "Inverse" seems to be the most ...
0
votes
0answers
26 views

Is it possible to convert nerve signals or electrical pulses into a readable format? [duplicate]

Can we have the technology to recreate the informations (like images, sounds etc.)[as in the form of signals in our mind] from human mind to human understandable format? In short, can we read human ...
1
vote
0answers
28 views

Are there any species which can live off rocket fuel and cosmic radiation? [on hold]

It was recently reported that cosmonauts have found sea plankton on the outside of the space station. Space agencies are nowadays very careful to sterilize their interplanetary landers, and orbiting ...
45
votes
11answers
10k views

Can humans survive without consuming life? [on hold]

Some people say that it's awful that humans eat animals. They feel that it's barbaric, because you're killing life and then on top of that, you're eating it, and that you should eat vegetation ...
0
votes
0answers
15 views

Bipolar minus end directed molecular motor

like the title explains I am looking for a minus end directed bipolar molecular motor. Any ideas? Best wishes.

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