0
votes
0answers
6 views

Pharmacology: Drug Administration

Why is the enteral route considered to be the safest way to administer a drug into the body?
3
votes
1answer
13 views

Is it essential to add cholesterol to agar or liquid culture for C. elegans?

In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious ...
0
votes
1answer
26 views

Why are human children more likely to get lice (nits) than adults?

By my own experience, lice seems to be prevalent in the hair of human children, but not in adults. My question is Why are human children more likely to get lice than adults? Assumptions: Can be ...
-1
votes
2answers
39 views

Do non-stick Teflon coated frying pans cause cancer? [on hold]

Is it true that using Teflon-coated pans contain carcinogens, and if so, can they be consumed through the food cooked in them? E.g. The deadly toxins from non-stick frying pans
3
votes
3answers
49 views

Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
2
votes
2answers
25 views

Premise of life cycle synchronization between predator and prey

While reading about the predator satiation hypothesis of the periodical cicadas' 13/17 year life cycles, I started wondering about its premise. By the way, I understand the math behind the prime ...
1
vote
0answers
17 views

Evolution of sexually concordant genetic variation on the X-chromosome

It was theorised in 1984 that sexually antagonistic genetic (SA) variation should more easily evolve on the X-chromosome. This is partly because female beneficial/male deleterious mutations face less ...
0
votes
1answer
29 views

Spermatogenesis; maternal homologs

What is the probability that, in an organism with a haploid number of 10, a sperm will be formed that contains all 10 chromosomes whose centromeres were derived from maternal homologs? I have ...
3
votes
1answer
19 views

Does isolation have any effect upon mouse behavior?

I work with a lot of mice. I don't do any behavioral analysis, I just inject DNA or RNA and do imaging assays. However, I have noticed some effect of isolation on mouse behavior. Normally, the mice ...
1
vote
0answers
22 views

Pharmacokinetics: why do certain drugs follow zero-order kinetics?

I understand that alcohol and phenytoin are two examples of drugs that follow zero-order kinetics. Why do these two particular drugs follow zero-order kinetics as opposed to first-order kinetics?
2
votes
0answers
23 views

Genome-wide methylation analysis: sources of technical error?

We are doing a genome-wide analysis of methylation via bisulfite sequencing for an insect species. Previous experimental techniques have suggested the presence of methylation in this organism, ...
3
votes
1answer
146 views

Microsatellite shifts (peak calling) GeneMapper! Thesis help!

I'm a masters student attempting to conduct a parentage analysis on a population of fish for my thesis. My advisor and post-docs haven't been very helpful, so I need some help! I have dinucleotide ...
0
votes
1answer
31 views

Are there other methods besides surgery and hormonal treatments to help undescended testicles? [on hold]

I've been doing some reading on undescended testicles and common treatments. It looks like the most common treatment for a baby is to give it time to see if the testicles drop on their own. If not, ...
0
votes
0answers
11 views

How are isopotential maps computed from EEG channel data

I have some EEG recordings of $1$ minutes, with $500$ samples per seconds and $63$ channels. For each recordings, this effectively yields a $30,000\times 63$ matrix $M$. How do I extract isopotential ...
3
votes
1answer
22 views

What is the best current understanding of how yeast transformation works?

I would like to get myself up to speed with what is currently known to science about yeast transformation. Specifically, transformation of plasmids and linear DNA fragments. I am particularly ...
4
votes
0answers
33 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
0
votes
0answers
10 views

Does the Y chromosome carry structural information, or only switches + traceable ID?

The Y chormosome almost doesn't crossover with the X chromosome, and so the evolution of anything it encodes must be much slower, because by the Hill–Robertson effect beneficial mutations must occur ...
3
votes
2answers
46 views

Does osmosis take place in prokaryotic cells?

As far as I know, osmosis occurs in Eukaryotic cells, and I'm wondering if it could take place in prokaryotic cells too.
0
votes
1answer
47 views

Can a guy be turned into a girl near perfectly? [on hold]

I have always been told that during gender realignment there is some damage to the body which is why even if you had all the money in the world you can't keep changing between male and female. ...
2
votes
2answers
33 views

What is a membrane potential?

I know this may be silly, but I am confused to what a membrane potential actually is. I understand that at resting membrane potential is -70- -80 mV. But what does that exactly mean and how does this ...
4
votes
1answer
66 views

What is this creature?

Since it has six legs, I believe it's some sort of insect, but I could be wrong. I spotted it in my Central Texas backyard (Austin area). It was very slow and not at all skittish. For size ...
1
vote
2answers
69 views

Can viruses that normally infect eukaryotic cells also infect bacteria?

Can standard viruses infect bacteria? I'm not speaking of bacteriophage but typical RNA and DNA virus such as influenza and Ebola.
1
vote
1answer
13 views

can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?

Dear fellow biochemists, I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore ...
0
votes
1answer
41 views

Does E. coli survive at any pH level?

Does E. coli survive at any pH level? If I was to incubate it in agar of different pH, would it still form a bacterial lawn as it's called?
0
votes
0answers
15 views

Is there a natural defense against an increase in E.Coli level in natural water systems?

I was recently on an Oceanology coliform collection vessel in the Long Island Sound and I found out that human waste contains E.Coli and when not properly treated the bacteria can run off or ...
3
votes
2answers
314 views

19th century Latin species description

While reading a 19th century text of new species description, I'm confused by some Latin phrases. The case here is: Corp. long. 8 poll., caud. 9 poll. I guess 'corp.' and 'long.' are ...
1
vote
1answer
38 views

Modelling how much cadmium a cell will be able to chelate with phytochelatins

I am involved in a synthetic biology project where I have to do modelling with ODEs. The biological process I want to model is the chelation of Cd2+ with phytochelatins, to know how much can be ...
0
votes
0answers
26 views

Is it possible to convert nerve signals or electrical pulses into a readable format? [duplicate]

Can we have the technology to recreate the informations (like images, sounds etc.)[as in the form of signals in our mind] from human mind to human understandable format? In short, can we read human ...
2
votes
1answer
43 views

NMR spectroscopy detection methods for proteins

While studying, I've stumbled upon a table comparing the sensitivities of different detection methods in NMR spectroscopy: "Direct", "DEPT" and "Inverse", where "Inverse" seems to be the most ...
1
vote
0answers
28 views

Are there any species which can live off rocket fuel and cosmic radiation? [on hold]

It was recently reported that cosmonauts have found sea plankton on the outside of the space station. Space agencies are nowadays very careful to sterilize their interplanetary landers, and orbiting ...
0
votes
0answers
15 views

Bipolar minus end directed molecular motor

like the title explains I am looking for a minus end directed bipolar molecular motor. Any ideas? Best wishes.
1
vote
1answer
18 views

How to identify the genes that distal enhancers pair?

I am writing a project proposal and I have to talk about this problem: how to identify the genes that distal enhancers pair? I am really new to this topic and I don't know what it is all about. I ...
3
votes
1answer
16 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
-2
votes
1answer
29 views

What prevents this blue lobster from becoming dominant?

Like everyone else in the world I saw the blue crustacean from becoming dominant? I would think the blue camo would be an evolution advantage.
2
votes
0answers
17 views

What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
2
votes
1answer
25 views

Enzyme Assay, absorbance change to concentration change

I have a few issues with this question. An enzyme assay is performed and the kinetic data graphed. The y-intercept of a Lineweaver-Burk plot is 0.6 min/ΔA. The substrate being assayed has a molar ...
2
votes
2answers
495 views

What happens if a person has the right side of the body totally removed (including the brain)? [on hold]

I guess humans could live without just the right lobe of the liver or the right brain. Could a person without the whole right side of body survive? The left brain controls the right side of the ...
0
votes
1answer
35 views

Global Acidification or Warming [on hold]

Which poses the greater threat to ecology Global Ocean Acidification or Warming?
3
votes
1answer
41 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
3
votes
1answer
55 views

Main mechanism of the evolution of sexual dimorphism

Sexual dimorphism is very common and (normally) requires the evolution of sex-specific genetic variation/regulation so the genetic correlation between the sexes can be <1. Is there consensus as to ...
1
vote
1answer
20 views

How long does it take for commercial fertilizers to break down? [on hold]

Sometimes we buy carrots that are abit bitter, it's the taste of fertilzer. Once i put a tomato in the fridge, and when i ate it, it was bitter like shweppes, and i figured that was fertilizer also. ...
1
vote
1answer
17 views

Calories burn rate calculation — scientific studies

There are many online calculators for measuring walking/running/bicycling calories burn rate. They differ in input parameters like some might need my weight as well as height and some don't. Almost ...
1
vote
2answers
22 views

Deduce length order of 4 decapeptides based on the Ramachandran plot

I have stumbled upon the following past exam question: 4 decapeptides A, B, C, D, made only of glycine residues, have different conformations, and the phi and psi angles of individual peptides ...
7
votes
2answers
46 views

Is mammalian vision processed as a sequence of frames?

I often read that people believe that human vision has an inherent frames-per-second rate (FPS) that causes stroboscopic effects - such as seeing the spokes of a rotating wheel apparently rotating at ...
2
votes
2answers
30 views

Western Blot: Fischer ECL substrate 2:1 instead of 1:1?

i recently developed a b-actin-ab-incubated PVDF-WB-Membrane with the Thermo Scientific Pierce ECL Kit. According to the manufacterers orders i mixed Reagent 1 and Reagent 2 1:1. But unfortunately i ...
1
vote
2answers
39 views

what is the modern, molecular explanation of independent assortment of alleles?

in case it isn't obvious i'll begin by stating that i'm a layman, trying to understand something about this subject from reading books. the books i've looked at so far give murky and confusing ...
-1
votes
0answers
43 views

Is there any evidence that soft tissue can be preserved for 65m+ years and if not what hypotheses exist to explain it?

I read an article on howstuffworks.com and they mentioned how we have found soft tissue in dinosaurs. According to the article even Mummies and Mammoths have no soft tissue left. Is there any evidence ...
0
votes
1answer
10 views

Rhizosphere vs. Endorhiza?

In microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In this case I ...
1
vote
2answers
22 views

Does bioluminescence communcation occur on macroscopic level?

Some bacteria are known to communicate using bioluminescence (quorum sensing). Do we observe the same phenomena in any macroscopic species?
1
vote
0answers
15 views

Given a microscopic image of a plant, is it possible to determine its age?

Given a microscopic image (let's assume a standard brightfield image) of a plant, is it possible to determine its age?

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