0
votes
0answers
6 views

What is the concentration of leptin in white adipose tissue in mice?

I am trying to perform an ELISA experiment and for this I need to know the physiological concentration of the hormone leptin in white adipose tissue in mice.
1
vote
1answer
78 views

Beetle with long, slender iridescent green body and orange legs

I recently saw this beetle while on a trip in central Texas, USA (in a rural area just before sundown). The body was about 2-3 inches long (compare to nearby chair leg and floorboards) and had a long ...
1
vote
0answers
13 views

How many animal species are larger than humans?

I sometimes think about just how huge humans are in comparison to most animals. Even compared to a squirrel, which is really a pretty large organism in the grand scheme of things, humans are hulking ...
1
vote
1answer
15 views

Choosing the best assay for my experiment?

Which of the following assays can be used to determine whether cell death specifically due to apoptosis has occurred in a given cell sample? Choose all that apply. 1) WST-1 Assay 2) Caspase ...
2
votes
0answers
42 views

What is the name of this bird?

Does anyone know the name of the bird in the pictures? It was found in the south of Spain in the month of June. (it is certainly neither a house sparrow nor a swallow nor a robin). Thanks for the ...
2
votes
0answers
21 views

Is perceived time compressed or expanded while dreaming? (or does it match reality) [on hold]

I often dream long extended dreams. Sometimes I wonder if the timeline of the story of my dream occurs compressed, in a moment, or other times extended slowly over a long duration. Is there any way ...
0
votes
0answers
16 views

Design arbitrary degenerate primers (with non-binding criteria)

I would like to design a number of arbitrary degenerate primers (primers with variable bases, e.g. NGATWGCTSATNGC) for a TAIL-PCR. I would like to be able to ...
0
votes
1answer
37 views

Why does my anti-ubiquitin antibody visualization not work on my PAGE gel?

I am using 2D gel electrophoresis to visualize polyubiquitinated proteins. However, while I can see actin and heat shock protein using when appropriate antibodies, I cannot visualize them using ...
8
votes
2answers
555 views

Linear and Circular DNA

So school has taught us that eukaryotes have DNA that is linear and inside of a membrane - called the nucleus. And that prokaryotes have circular DNA that is free floating inside of the cell. We ...
-2
votes
0answers
21 views

Plankton growth as a function of temperature [on hold]

I have a data set like folow Time-Temperature(0C)-Species_1(individuals) 01-T1-S1 02-T2-S2 n-Tn-Sn Now I want to caculate growth of species_1 as a fuction of temperature. How I can do that from the ...
6
votes
1answer
250 views

How bad is ethidium bromide in your plasmid for downstream applications?

I want to transfect Adipose Derived stem cells using a clone of my own. My problem is that I get a lot of contamination (not only genomic, but due to the nature of the technique, I get undesired ...
1
vote
1answer
45 views

What concentration of table salt is needed to kill bacteria in sweaty gym clothes?

My gym clothes retain an unpleasant musk even after washing them with warm water and detergent. I've experimented with soaking my gym clothes in salty water before washing them. My hypothesis is that ...
7
votes
2answers
1k views

Is this a crayfish hole? or wasp nest?

I'm suspicious that it might be crayfish, which I'd think was insane (I still kind of do), if I hadn't found a dead one around my house earlier this summer. Is that definitely what it is, or do I ...
1
vote
2answers
101 views

What is a substance that is safe to eat, not absorbed and not metabolized?

I would like to know whether there are substances that are safe for humans to consume, are not absorbed and do not interact with any digestive process. So, food is disqualified because although it ...
2
votes
1answer
39 views

(How) Does the human digestive system process petroleum jelly, aka petrolatum?

Does the human digestive system processes petroleum jelly? In other words, does it pass through the digestive system without any interaction, or is it absorbed and/or metabolized?
0
votes
1answer
38 views

Which ORF will be translated

I've a question about ORF and translation into protein. Say I have an RNA transcript that contains two ORFs, one in phase 1 ; one in phase2 ; as: ORF number 1 in reading frame 1 on the direct ...
-2
votes
0answers
35 views

Buccal vaccine for viral infection of MALT

What would be the effect of a daily (repeated for several months) buccal or sublingual vaccine in the form of a deactivated virus - say HSV1 - to a person already infected with it? Whilst also ...
0
votes
1answer
21 views

What does the “cov” mean in a velvet assembler generated contig name?

A exmaple of a contig name generated by velvet assembler: NODE_127_length_39203_cov_244.873016 What does cov_244.873016 mean? ...
0
votes
2answers
91 views

Why a striped baby is not the result of a white and a black parent? [on hold]

Someone asked me: If a striped cat is the result of a white and a black cat, why the result of a white woman and a black man is not a striped child? I know the answer. I also know that the ...
0
votes
0answers
25 views

What are the effects of diuresis and limited water intake? [on hold]

What are the effects on the kidney when taking a loop diuretic and drinking too little water? Loop diuretics like furosemide cause elimination of water by inhibiting water reabsorption in the nephron ...
0
votes
0answers
12 views

Upcoming advancements in bio technology [on hold]

What are some upcoming advancements in biotechnology which have a drastic impact on life of common man. For example in mobile technology we saw drastic advancement in mobile technology like touch ...
21
votes
1answer
3k views

What is it that creates that wonderful smell after a fresh rain?

I've heard that the wonderful smell of a fresh rain is actually chemicals released from the trees and grass and other plants. What is the process that allows these chemicals to be released? What ...
4
votes
1answer
51 views

What is the scientific name of this evergreen plant?

This plant is found in India. It is an evergreen plant. Would love to know the scientific name.
2
votes
2answers
49 views

How do researchers map root systems of plants?

For example, here is a picture of the root systems for some prairie grasses: (Click image for higher-resolution PDF) from: http://www.conservationresearchinstitute.org/educational-offerings.html How ...
-1
votes
0answers
22 views

Why haven't humans 'evolved away' aging? [duplicate]

Here's a weird question: why haven't we evolved to live longer, as opposed to living longer through healthcare etc.? It makes sense that if a person lives longer, they have more offspring, ergo in the ...
1
vote
0answers
11 views

Application of Latin names for Taxonomy

The current taxonomy has family names like Canidae and Felidae. But Felidae has sub families like Felinae and Pantherinae. Roaring cats and non-roaring cats. My question is based on the fact that we ...
1
vote
1answer
34 views

How do I find the sequence of the phage BxB1 gene?

Specifically, I'm trying to find the sequence of BxB1 and its att sites. I have success finding the att site sequences, but I'm having a bit more of an issue with finding the sequence of the actual ...
-1
votes
0answers
43 views

Do I still have a chance of growing taller? [on hold]

I'm 20 and 5'6". My dad is 6' and my mom is 5'7". I don't believe I went through puberty abnormally early or late. My back molars still have not grown in, and my beard hasn't filled in completely (no ...
4
votes
1answer
86 views

How do steroids travel in the bloodstream?

Cholesterol, which is hydrophobic, cannot simply travel in the bloodstream, and instead is enclosed in lipoprotein (LDL, HDL). Steroids, which are derived from cholesterol, are also hydrophobic ...
4
votes
1answer
35 views

Forward versus backward numerical simulations in population genetics

My question is closely related to this post. There are a number of existing platforms to perform numerical individual-based simulation in populations genetics. An almost exhaustive list of such ...
3
votes
0answers
44 views

Why do some medicines induce sleep? [on hold]

I have seen lot of people sleep or say that they are feeling sleepy after taking some medicines. Why? I mean, if a medicine acts on some cell of the body because that cell has got its receptors and ...
5
votes
0answers
29 views

What have the L-systems (Lindenmayer systems) been actually used for in biology?

Wikipedia: An L-system or Lindenmayer system is a parallel rewriting system and a type of formal grammar. L-systems were introduced and developed in 1968 by Aristid Lindenmayer, a Hungarian ...
-2
votes
0answers
23 views

How hot can coffee be for it to burn the tongue? [duplicate]

How hot can coffee get before the tongue will get burned? I want to know an accurate temperature. Specifically coffee. I felt that the other question didn't have a definitive answer.
4
votes
1answer
64 views

Identify bee in photo (found in Utah, USA)

A friend took this photo of a bee in his back yard in central Utah (© Jon Mott, CC-BY 3.0). I have no formal training in systematics or entomology, so to me everything looks like the bees' poster ...
1
vote
0answers
46 views

Where do the H+ ions come from in light reactions?

In the light-dependent reactions of photosynthesis, H+ ions pass to the thylakoid space. Where do these protons come from?
1
vote
0answers
12 views

Calculating the canopy size (crown) of a newly planted tree at a future date

Assuming that one knows the maximum area of the canopy and the age of the tree when it achieves this. And given an initial canopy size from the nursery. How would I calculate the canopy size at a ...
38
votes
1answer
3k views

What could cause a forest of bent trees?

While hiking on the northern Idaho-Montana border, I encountered a large area where virtually every tree is bent at the base in the downhill direction. Only the very largest and very smallest trees ...
3
votes
2answers
243 views

What does this equation about DNA replication mean?

Could someone help me understand this equation please? I found it in a paper which said that it was DNA replication, but why? $\ce{dNTP + dNMP_{n} -> dNMP_{n +1} + PPi}$ I found that dNTP means ...
5
votes
1answer
43 views

Does the anatomy of our hand result in (computer) mouse-accuracy that varies by direction of movement?

First off, I apologize if this isn't the right stackexchange sub-site for asking thsi question. I was torn as to where I should post this question: it could plausibly belong in Biology, User ...
2
votes
1answer
54 views

Can you recharge without sleeping? [on hold]

Sometimes it's difficult falling asleep for almost an entire night. You might feel like getting up and being productive instead, or 'pull an all-nighter'. Or you can try to simply lay in bed to relax ...
2
votes
1answer
58 views

Has anyone modelled calcium oscillations using Matlab software? [on hold]

I have been trying to model calcium oscillations using MatLab, but I cannot obtain the oscillation. I created a function where I put all the equations and used ode15s to call the function. I have ...
3
votes
1answer
36 views

How does tearing work?

I found the question about tear gas very interesting. Shigeta says there that crying helps the cells of the eye to wash themselves clear of the toxic components in the tear gas. Does non-emotional ...
3
votes
1answer
23 views

Crow branch pecking behaviour

I was walking through a small park when two crows started cawing at me, and followed me, flying from tree-to-tree as I walked. I speculate that this is a territorial or protective behaviour, but what ...
3
votes
1answer
22 views

Phosphorylation-Dephosphorylation : ATP-driven VS spontaneous

Is protein dephosphorylation most commonly spontaneous (without need of ATP)? I came across some papers that mention it, as well as the opposite case (ATP-driven), but I can't figure out which is ...
2
votes
0answers
21 views

What are all of the currently known variations of the G zipper motif?

The G zipper motif is found in transmembrane proteins at an above random frequency and there are models explaining how it might help with multiple transmembrane intra-membrane helix bundle assembly. ...
1
vote
2answers
67 views

Why do many DNA solutions contain additional compounds?

DNA solubility data in only water is scarce. A previous question asked for a quantification of DNA solubility in water. It seemed like it would be easily answerable, however isn't quite that simple ...
3
votes
1answer
19 views

Accuracy of best plant growth models?

Given a plant $P$, predict its height $H$ at time $t$ since planting given some environmental variables $E_1, \dots, E_n$. How accurate are the state-of-the-art models for such thing? What data is ...
7
votes
1answer
373 views

Toronto spider identification

I found it in Toronto, Ontario, during early June, 2015. When prodded it seemed to jump around, or at least to move very fast. I can also post a short video of it if needed.
2
votes
1answer
39 views

Animal identification (larva)

I found a tiny, white animal in my mouth on the morning of June 28, 2015, in Toronto, Ontario. Luckily I don't seem to have hurt it. I was eating some sunflower and pumpkin seeds at the time. I ...

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