9
votes
0answers
103 views

Using the IMGT/GENE-DB service to find RSS

I'm trying to get the data for the Human and Mouse 12 and 23 Recomination Signal Sequences (RSS), to run a classification algorithm on it. I'm not a biologist, so I apologise in advance for my ...
7
votes
0answers
121 views

How does Sodium Valproate cause neural plasticity

I have been reading a fascinating paper: Valproate reopens critical-period learning of absolute pitch 18 individuals were given Sodium Valproate (VPA) for a fortnight during which they trained on a ...
6
votes
0answers
47 views

B cell receptor editing

If a B cell contains two mu chains and two lambda chains and is self reactive can it go back and rearrange its kappa light chains? I'm not sure if it can only try to rearrange its lambda light chains ...
6
votes
0answers
72 views

effect of pupil responses on the electroretinogram

The electroretinogram is generally considered to reflect the retinal processing of a light stimulus, typically a photoreceptor response followed by secondary neuronal activity. However, I am wondering ...
6
votes
0answers
168 views

Additive genetic variance components from LMER in R

I've set up some dummy data in R which makes 40 genetically related lines, they are all siblings within a line so are genetically related by a factor of ½ thus additive genetic variance should be ...
5
votes
0answers
19 views

Why do humans hear better between 2kHz and 5kHz?

I've recently seen the following image: Source: https://commons.wikimedia.org/wiki/File:Hoerflaeche.svg Translation (from German): The upper boundary (Schmerzgrenze) is where it starts to hurt ...
5
votes
0answers
41 views

How to define “Quasifixation” in continuous approximation of finite population?

Background Many models including the famous very first models derived by Sir Ronald Fisher in his early career, assume infinite population size. In an infinite population, an allele can rise in ...
5
votes
0answers
48 views

Hill's function for translational regulation

Transcriptional regulation is generally modeled as a Hill's function (similar to Michaelis-Menten Kinetics): $$\frac{dm_X}{dt}=\alpha _{m_X}.\frac{R}{K+R} -\beta _{m_X}.m_X$$ Where $m_X$ is the mRNA ...
5
votes
0answers
175 views

Cytoplasmic determinants - protostomes and deuterostomes

Cytoplasmic determinants are spread unevenly in the egg, and so when embryo starts forming (cells start dividing), the determinants are also unequally divided between cell. This unequal distribution ...
5
votes
0answers
103 views

Model for fluctuating selection

Is there any mathematical model to predict the behaviour and long-term consequence of counter-acting selection at different time scale? For example, let's consider the bi-allelic gene A, with alleles ...
5
votes
0answers
53 views

Honeybee decision process: new food, new home, new threat

I have heard that honeybees send out scouts, then based on the scout reports more bees go then come back and report and so they are supposed to be efficient decision makers. Can you give as complete ...
5
votes
0answers
273 views

Reason for seeing grid-like patterns when strobing a solid black-white image?

I was trying to do some stimulation for SSVEPs by strobing a solid black square and a solid white square. Weird thing was, I ended up seeing weird grid like patterns that looked like this, always of ...
5
votes
0answers
98 views

Do mammals develop tolerance to anticholinergics?

I know that first generation H1 antagonists, commonly known as antihistamines have anticholinergic effects. Their sedative side effects go away due to tolerance, but as for their anticholinergic side ...
5
votes
0answers
126 views

What temperature should mammalian B-Cells be stored at outside of the incubator?

I'm working with murine B-cells. The general protocol is to keep cells on ice to keep them from dying but I've noticed that it makes these cells aggregate and precipitate out. I've heard suggestions ...
4
votes
0answers
21 views

Why should a tumor look like a crab?

Origin of the word cancer The disease was first called cancer by Greek physician Hippocrates (460-370 BC). He is considered the “Father of Medicine.” Hippocrates used the terms carcinos and ...
4
votes
0answers
46 views

Why do some internal organs regenerate?

I have been reading about the human liver and zebra fish heart muscle having the ability to regenerate. It seems to me that these organs have very little chance to become damaged or worn out. At the ...
4
votes
0answers
34 views

What are parental antagonism & parental conflict?

I've been reading the chapter by Haig et al in The Genetics and Biology of Sexual Conflict. I think this chapter has them quite unclearly defined. Could anyone describe these two phenomenon more ...
4
votes
0answers
23 views

Mechanisms of Gene*sex / Genotype*sex interactions

I'm looking for suggestions of the mechanistic level at which genotype/gene * sex interactions can occur. These give different phenotypes from the same geneotype dependent on the sex they are ...
4
votes
0answers
13 views

Would constant light or fading light be registered as more wakefulness promoting by the Supra Chiasmatic Nucleus?

During the fall and winter season, a number of people are experiencing Seasonal Affective Disorder. One of the way it has traditionally been treated is with a dedicated lightbox - a very high ...
4
votes
0answers
87 views

What is this fuzzy, black fungus that grew on my plates in the 4⁰C room?

I often find the fungus below growing on my (ostensibly) sterile plates in the 4⁰C room. Presumably it takes a few days to reach this size. The colony looks puffy and dimpled in the middle, like a ...
4
votes
0answers
49 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
4
votes
0answers
36 views

How was the cama's life expectancy computed?

A cama is a hybrid between a male dromedary camel and a female llama. The first cama was born on January 14, 1998, yet on the Wikipedia page it is said that a cama's life span is 30–40 years. How was ...
4
votes
0answers
29 views

Why doesn't Clopyralid damage cool season lawn grasses?

I use Clopyralid (3,6-dichloro-2-pyridinecarboxilic acid) to kill broad leaved weeds in lawns. From my understanding, it works by mimicking an auxin which affects plant growth. Naturally this pulls ...
4
votes
0answers
42 views

How does the frequency of a visual stimulus affect the steady-state visually evoked potential?

I want to make a project for EEG signal processing, and in my research I found the concept of SSVEP, which means that if you have a stimulus with low frequency applied to the eye, the electrical ...
4
votes
0answers
84 views

How do duplicate brain regions (ex: left/right amygdaloid body) operate together?

I frequently hear talk about parts of the brain like "Amygdala" or "Hypothalamus", so I looked them up in an app called "essential anatomy". What I see is that there's mirror symmetry, and most of ...
4
votes
0answers
88 views

Does Lucid Dreaming decrease the regenerative effect of sleep?

After watching this SciShow video on sleep, I wonder about lucid dreaming. Specifically: Does lucid dreaming and associated directed agency within Lucid Dreaming decrease the regenerative effect of ...
4
votes
0answers
83 views

At which step apply a detection filter when preprocessing microarray data? (Before/After normalization, batch effects removal)

I am trying to understand what is the best practice when preprocessing microarray data using a detection filter (on scanner p-value). Suppose I have a microarray dataset that I have to normalize with ...
4
votes
0answers
157 views

Genetic expression in interspecific hybrids

Referring to interspecific hybrids, I have the following two questions:- Quoting from wikipedia:- The offspring of an interspecific cross are very often sterile; thus, hybrid sterility ...
4
votes
0answers
51 views

Does DHA increase the effects of clarithromycin?

I've read on this publication that every drug that induces CYP3A can reduce the effectiveness of clarithromycin. Clarithromycin inhibits CYP3A. But it does not say anything about the opposite ...
4
votes
0answers
67 views

What is the limit of time resolution in fMRI?

I was reading about a few studies on estimating functional connectivity between brain areas using fMRI signals. However, as far as I know that fMRI has a very poor time resolution, roughly in the ...
4
votes
0answers
59 views

Does preservation in ethanol alter leaf litter mass?

I have benthic samples that were collected with an Ekman dredge from some small ponds. The samples contain quite a bit of coarse particulate organic matter (CPOM, basically dead leaves). I would ...
4
votes
0answers
85 views

What controls leaf senescence in deciduous tree species, and how can I predict it?

If I want to predict litterfall, what data do I need to collect, and what statistical model might I use? For example, I might use the following coding to record phenological stage every other day, or ...
4
votes
0answers
98 views

Comparative cost of RNA-seq vs sequencing full length cDNAs

I am in the process of assembling and annotating the genome of a non-model organism, using almost exclusively short read (paired-end Illumina) data. Throughput is one obvious benefit of these data ...
4
votes
0answers
98 views

Terminology question: the scope of an allele in an organism

Let us consider a gene FOO with novel type foo. If I were discussing an organism that has inherited foo in every cell during classical zygote formation, then I would ordinarily just say that the ...
4
votes
0answers
69 views

What is the frequency of double-hets between parent and child?

Say both parent and child are genotyped for all SNPs. In this setup we are only looking at variant positions between one parent and child - so neither parent nor child are homozygous reference (no ...
4
votes
0answers
55 views

Does a mydriatic drug neutralize the action of a miotic?

If a person were administered a mydriatic, would the subsequent application of a miotic neutralize the action of the former? If the sequence were reversed would a mydriatic neutralize the effect of a ...
4
votes
0answers
69 views

effect of background selection on promoter regions compared to distant enhancers?

Has anyone looked at the effect of background selection on the levels of conservation of promoter regions compared to distant enhancers? Do promoter regions have a higher conservation due to ...
4
votes
0answers
27 views

What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
4
votes
0answers
101 views

Dimensionless number for blood volume

Comments from the question How is the blood volume of a living organism measured without killing it? by @Nico discussed that the time of blood recirculation scales with the size of the organism. I was ...
4
votes
0answers
44 views

Serological assays not detecting native proteins

Is there anyone out there who has done much work with serological assays? We have antiserum for a manufactured viral protein but no luck so far getting it to detect native protein (unless today's ...
4
votes
0answers
110 views

If D1 receptors stimulate adenylate cyclase (through GPCRs) and D2 receptors inhibit it, then why do mutations in both have similar effects?

D1 and D2 both refer to specific types of dopamine receptors. I'm sure it has something to do with the fact that the D1 receptors are in regions different from D2 receptors. I know that adenylate ...
3
votes
0answers
38 views

Single long axon vs serial neurons

Based on the comments in this post and also this chat. For discussions and speculations please comment in the chat. The basic question is what is the advantage of having a single long axon such as ...
3
votes
0answers
30 views

Statistical method for characterizing the relationship between body mass and metabolic rate

There is a dataset that contains body mass ($x$) and metabolic rate ($y$) from many different organisms. It is common to fit the data to the model of the form $y=ax^b$ and estimate the parameters $a$ ...
3
votes
0answers
22 views

Circulating factors affecting human health/longevity

Circulating factors present in young mice have been shown to promote rejuvenation of aged mice, suggesting that tissues have inherent capabilities to regenerate, and circulating factors may be ...
3
votes
0answers
22 views

Why are most transcription factors enhancing the expression rather than repressing?

One can classify the effects of Transcription Factors (TF) on gene expression into two types: it either enhance or repress the gene expression. I have always been told that most of transcription ...
3
votes
0answers
22 views

Well to well variation in thermal cycler fluorescence?

We have an old BioRad ICycler Thermal Cycler with MyIQ single color fluorescent detector. While it's meant for RT-PCR, we've been using it for melt curves and binding assays for different types of RNA ...
3
votes
0answers
118 views

Evolutionary reasons why you cannot tickle yourself but masturbate

There are obviously good reasons that explain why you cannot tickle yourself (see e.g. here). This got me thinking why it is possible to masturbate... Wouldn't it make more sense to not being able to ...
3
votes
0answers
36 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
3
votes
0answers
35 views

How many eukaryotes are there on Earth?

I have been reading: William B. Whitman, David C. Coleman, and William J. Wiebe, "Prokaryotes: The unseen majority", Proc. Natl. Acad. Sci. USA 95, pp. 6578–6583, June 1998. [Full Text] [PDF] ...
3
votes
0answers
29 views

Biological age of grafted plants

Suppose you graft a piece of an existing 'old' plant. Will it continue to grow having the same biological age as its parent? I.e., would it die at the same time as its parent? Or would the process of ...

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