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If protein 1 contains a glutamate near the binding site with protein 2 and is close enough to the lysine on protein 2, it may be possible to link the glutamate to the lysine through EDC coupling. EDC is 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, which reacts with carboxyl groups to form an "active ester". This ester is highly reactive with primary ...


There are general guidelines for cross-linking protein-protein interactions, but specific parameters (i.e. cross-linker sub-types, spacer length, linker concentration, reaction pH and temperature) are often determined experimentally. Common protein cross-linkers target reactive functional groups that are more likely found on the surface of the protein than ...

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