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The rapid screen usually tests the oral mucosa by swabing the inside of the cheek and checking for the presence of anti-HIV envelope antibodies. Although the precise target of these antibodies seems to be kept propriatary, my guess would be the detection of either p24 antibodies or envelope antibodies that bind the V3 region. Regardless, the point is they ...


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Clarify, when you say "antibody" do you mean a monoclonal antibody (where every antibody is identical, and therefore a pure population), or do you mean a polyclonal antibody (which is a mixture of antibodies that all recognize the same antigen, but may each be recognizing different epitopes on the antigen)? A monoclonal Ab (mAb) recognizes a single epitope. ...


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TL;DR Specificity of antibody can be not superb. It can give false-positive results in up to 90% of experiments. However, some essay are 90% specific. That is an interesting question and there sure a lot of research done to understand specificity and strength of binding of antibody to its target. First of all, let me say that antibody-epitope interaction ...


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You can compare directly coupled antibodies to the classical indirect primary-secondary systems: Directly coupled antibodies Advantages: quicker workflow as only one antibody is used eliminates the chance of a cross-reacting secondary antibody (possibly less background) possibility to use differently labelled antibodies on the same blot Disadvantages: ...


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The reason why the secondary antibody only recognizes the primary antibody is relatively simple: It is directed against the Fc-part of the primary antibody. As this part is species specific (but constant between all antibodies from one species, hence the name constant) a secondary anti-mouse antibody is directed against all mouse antibodies. The general ...


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Antibodies are very specific on what they recognize (this is called epitope that is the small part of the antigen that is recognized by a specific antibody), and secondary antibodies are made to attach to a specific part of the primary antibody - you can achieve this with monoclonal ABs. Antigens can be recognized by many antibodies (because they can have ...



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