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Like any other protein, antibodies will aspecifically bind nitrocellulose or PVDF membranes, but any other protein present in your antibody preparation will also do. Depending on the antibody class, more specific binding can be obtained with protein A or protein G, that recognize the Fc domain. It's usual to have protein A/G immobilized on a stationary ...


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It is unlikely that an antibody would bind another antibody within just the human species. Human antibodies which bind to the human Fc portion cannot exist as they would be filtered by self tolerance mechanisms (as these B cells would be chronically activated or would the T cells that would be required for costimulatory help allowing the B cells to class ...


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The short answer is that antibodies are proteins like any other, and if an antibody from a foreign source is injected it can result in an immune response. This is, of course, exploited in the production of secondary antibodies for use in research (e.g. goat anti-rabbit IgG is a goat antibody that recognises rabbit IgG). Because of this, therapeutic ...



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