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For the generation of Fab-fragments antibodies, (possibly genetically modified) which can be made in large quantities by cells or animals, are used. Antibodies as a whole are not synthesized. The Fab fragment is obtained from antibodies using the enzyme papain, which cleaves the antibody over the disulfide bonds in the hinge region. This results in two Fab ...


FAbs are genetically engineered and not organically synthesized as such. This PNAS article nicely describes the construction of a phage (virus) library to generate a large amount of different FAbs and screen for FAbs with the wanted epitope (antigen) affinity: http://www.pnas.org/content/95/11/6157.short

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