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I'm a pharmacy student and I can try to explain how they do to find new drugs. In fact, there are many diferent ways to do so, it depends of what kind of new drug you are searching for. Sometimes you're working on a disease which the pathological molecular mechanism of action are well-known and where many drugs are already effective. In this case, the ...


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An assay may be thought of as a trial or test that is designed to quantitatively determine the amount of a substance in a sample. Thus a biochemist might use a protein assay to quantitatively determine the amount of protein in a sample, or a pharmacologist might assay a sample to quantitatively determine the amount of drug present, or an enzymologist ...


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In my experience, reuse of NADPH stock solutions maintained at pH 7.0 or above is perfectly acceptable. Taking an absorbance spectrum every half-hour or so will probably show that definitively. However, NADPH is VERY unstable in acid, leading to a 'bleaching' of the absorbance at 340nm. Try measuring a rate at pH 6 or below, 340nm, with 100 micromolar ...


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Using different wavelength for reference give some advantages. You can measure a baseline of each well. All well might not have the same baseline because each well might not be the exactly same as others. In addition, you could make scratches on it, you might touch the bottom, or you get some dirt from your bench. And this can be corrected by A540 in your ...


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So, your blank is everything but the enzyme? That is, substrate and DNSA with 100 uL water? If yes, measure, as negative controls, substrate alone and DNSA alone. If one of them is also highly absorbing light, it may be contaminated, and a new vial should be ordered. If both are highly absorbing, it may be the water you are using, or the spectrophotometer. ...


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Background: There are a lot of recipes to extract protein from human tissues out there, but all of them boil down to one thing; preserving the tissue proteins as much as possible while obtaining a reasonable yield for downstream applications, using extraction buffer, extra techniques, and by adding protease inhibitors ELISA is one of the those ...


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It's hypothesized that planar cell polarity is many cases functionally associated with asymmetry of (primary) cilia (Axelrod (2008)). So, the phenomenon of primary cilium migration could be not that infrequent. One example is the development of ependymal cells in brain ventricules: They are multiciliated and the cilia are motile, but ciliogenesis starts from ...


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Perhaps you're looking for these hanging inserts, for example: "Millicells"


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Apparently poly(ethylene oxide) is a biologically interesting polymer, with minimal protein interactions. The apparently means that I haven't tried it myself, but my polymer teacher told us so. http://www.sigmaaldrich.com/materials-science/material-science-products.html?TablePage=20204110 http://en.wikipedia.org/wiki/Polyethylene_glycol Nomenclature ...



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