New answers tagged assay-development
In my experience, reuse of NADPH stock solutions maintained at pH 7.0 or above is perfectly acceptable. Taking an absorbance spectrum every half-hour or so will probably show that definitively. However, NADPH is VERY unstable in acid, leading to a 'bleaching' of the absorbance at 340nm. Try measuring a rate at pH 6 or below, 340nm, with 100 micromolar ...
Top 50 recent answers are included