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Transformation underpins a lot of lab modern projects. This will be a very unsatisfying answer, and this wikipedia entry explains why. The time, and success, of bacterial transformation of competent cells varies greatly depending on a few things mainly: the length of nucleic acid sequence for insertion (or more accurately, the plasmid) the species of ...


3

Note: in the following I'm going to focus on E. coli, which is the best understood system for bacterial flagellar movement. It would be interesting to compare uniflagellate bacteria and bacteria that have a fixed number of flagella. However, I know about E. coli and I think there is something interesting to be learned just from them, so I'll continue. ...


7

Interesting question and it will only be possible to do some estimation. The problem is to know the exact bacterial composition as well as the mass of the bacteria. To start, it is necessary to know the number of bacteria in a defined amount of yoghurt. There are scientific publications on this topic, but the numbers in fresh produced yoghurt are in the area ...


4

According to this website, it is a mnemonic for "lambda excision". I have also found this usage in scientific literature (Harami et al., 2013). However, neither of these sources reference anything and I cannot find any defining paper. Almost all papers simply refer to it as lexA. As far as I can find (it's difficult to dig up these old papers), this is how ...


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I'd go with Lambda Excision A. Terms like lex or rec often stand for what'd be termed a mnemonic, where for example rec may stand for recombination, or umu for UV mutator. The naming conventions can be difficult. Edit: A 1981 study by Roger Brent and Mark Ptashne notes some data from initial studies that showed the lexA repressor downregulated a number of ...


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Pseudomonas bacteria are generally not difficult to grow: Rugged and opportunistic, Pseudomonas use a wide range of nutritional sources, even very simple nutritional environments without any organic compounds. - Sigma For specific species, you'll want to use a selection medium. Sigma-Aldrich has a number of commercial products for selection and I'm ...


3

Insulin is processed from proinsulin by folding the peptide and forming 3 disulfide bonds before cleaving the peptide in 2 positions, leaving 2 peptide chains held in place by the disulfide bonds. This process is summarized in this image: This only works because the peptide is packaged in a secretory vesicle during synthesis. This protects the peptide ...



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