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The answer to this is fairly complex, and it depends on whether you are talking about short-term or long-term changes to the microbiota. When talking about short-term changes, the answer is almost certainly a resounding yes. Any ingested nutrient will lead to an increase in the growth rates of bacteria capable of metabolising that nutrient, and will ...


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Use agar stubs: pour some Nutrient agar (or any suitable agar medium) in a test tube (~10% of the tube volume) and punch your inoculum halfway into it using a pipette tip or inoculation loop. Store it in 4⁰C. Glycerol stocks can be made and stored in -20⁰C also.



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