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12

Yes, plants! Plants are autotrophs. While Animals and fungi are heterotrophs. Have a look to the wikipedia articles. In short, autotrophs organisms are organisms that synthesize their own compounds from inorganic compounds. Heterotrophs organisms are organisms that synthesize their own compounds from organic compounds. Therefore, any multicellular plants ...


7

See this paper. They have studied RBP-protected sites in the entire human transcriptome by RNA-protein crosslinking followed by RNAse digestion and sequencing: PIPseq. Figure 1 of the paper shows distribution of protein protected sites in RNAs. They also correlate it with different regions of mRNA and its expression. They show number of protein protected ...


6

I've reproduced the diagram that you linked to. It shows the oxidation of a pair of thiols to create a disulphide. What is missing from this scheme is the accompanying oxidising agent. So for example this could be carried out without catalysis in a reaction with molecular oxygen, in which case hydrogen peroxide would be formed. So the electrons and protons ...


4

Your cells produce thymidine by converting uridine using an enzyme called Thymidylate Synthase. 5-Fluorouracil irreversibly inhibits this enzyme. Here is the structure of 5-Fluorouracil: Here is the mechanism the enzyme uses to make thymidine: It looks like the enzyme forms a bond to the uridine ring through a sulfur. 5-Fluorouracil probably inhibits ...


4

In principle, Meiosis is only the process in which the haploid egg or sperm are generated. Have a look at this figure, which shows Meiosis I and II (from the Wikipedia): During Meiosis I homologous recombination between homologous chromosomes can happen, the chromosomes are then distributed normally among the daughter cells. In Meiosis II the cells split ...


3

Embalming solutions for cadaver study differ from institution to institution (and are reportedly somewhat different from embalming for funerals, but I'm not sure of those details). Typically embalming solutions for cadavers meant to be stored indefinitely consist mostly of water with lesser but roughly equal amounts alcohol (usually methanol), gylcerin, ...


3

Yes, this is the effect of metabolizing ethanol and also the reason why excess amount of ethanol can lead to hypoglycemia. Ethanol is oxidized by the alcohol dehydrogenase to acete aldehyde. This reaction needs consumes one molecule of NAD+ per molecule ethanol oxidized: Ethanol + NAD+ <=> Acetaldehyde + NADH + H+ Acetaldehyde is oxidized by the ...


3

Fats include free fatty acids and their compounds with glycerol: mono-, di- and triglycerides. In a cooking context, fats refer to solid fats, and oils to fats that are liquid at room temperature. Most of them contain triglycerides. Fats belong to a broader group--lipids--, which are substances that are usually not soluble in water and usually soluble in ...


3

After some research, I could primarily find two tests for testing the protein content in dog urine. 1) The Urinalysis dipstick test (reference) which has a chance of giving false positive results (reference). Here is the link to a lab test done using the dipstick method and the results that the researchers got. A sample Dipstick (reference) 2) The ...


3

Anti-oxidants affect human metabolism by altering the redox states of the cell and redox-regulated functions and signaling mechanisms. The following quotes are from The Redox Stress Hypothesis of Aging (Free Radic Biol Med. Feb 2012) More recently, in a major conceptual shift, ROS have been found to be physiologically vital for signal transduction, ...


3

Really the question how does protein folding work? But let me answer your questions... 1) Very few proteins have disulfide bonds (usually secreted proteins) or really any covalent bond stabilizing the amino acid chain beyond the bonds that make up the polypeptide itself. Denaturation is only reversible in relatively few cases in fact. A few proteins, ...


3

coconut oil is composed mainly of medium-chain triglycerides, 1)it is smaller than other long-chain triglycerides so that it can be digested easily, 2)the digestion of coconut oil does not involve in the Pancreatic digestive enzyme system, 3) liver prefer use medium-chain triglycerides as the fuel sources 4) medium-chain triglycerides will convert to ...


2

Are you taking in an in vitro context for preventing protein denaturation after protein isolation from for example E. coli or are you more worried about proteins in the context of the whole cell? I'm no expert in the protein folding/conformation studies but from laboratory based prospective if you want to achieve denaturation for experimental purposes, you ...


2

DNA polymerase must catalyse the addition of 4 different nucleotides to the growing strand. This means that it cannot directly determine which base to incorporate at a specific point (how would it 'know' which base to incorporate and how it would it change its specificity for different bases). This means that the specificity for which base pair to ...


2

High-fidelity DNA polymerases have several safeguards to protect against both making and propagating mistakes while copying DNA. Such enzymes have a significant binding preference for the correct versus the incorrect nucleoside triphosphate during polymerization. If an incorrect nucleotide does bind in the polymerase active site, incorporation is slowed ...


2

Afaik you can describe the potentials between the two sides of the cell membrane using the Nernst-equation. Yepp wikipedia writes the same here. Membrane potential is a very important thing, because human cells require stable internal concentrations of Na+, K+, Ca2+, etc... in order to work properly. These concentration can depend on species, for example ...


2

The answer here lies in epidemiology and the pathogenic nature of the virus. Humans infected with Ebola have a range of recovery rates of 5-75%, meaning that most of those infected will not survive infection. Given the combination of preparedness factors at first recognizing a true outbreak in Ebola and viral load which had already been spread by the point ...


2

This is a draft. How do the pharmacodynamics of the NSAIDs differ - -? Merck's manual ch. 36 to start little bit at an introductory level: The anti-inflammatory activity of the NSAIDs is mediated chiefly through inhibition of biosynthesis of prostaglandins (Figure 36–2). Various NSAIDs have additional possible mechanisms of action, including ...


2

From doing a bit of research, I quote The various analytes have different stability in serum. Serum TLI and PLI are stable for up to 2 weeks at room temperature. However, serum folate shows limited stability at room temperature. Therefore, serum samples for folate analysis should be kept at 4ºC for not longer than 48 hours, but freezing is ...


1

The fundamental principle behind this is a "sensor" or the excitation-contraction coupling, regardless where you are. Let's consider the striated muscle and the cardiac muscle. The function of excitation-contraction coupling in skeletal muscle is as voltage sensor to tell the SR, "We have got an action potential; release Ca2+ for contraction". This is a ...


1

The sRNA mediated regulation in bacteria operates via diverse mechanisms. This case of sRNA competing with a mRNA for a protein is a passive kind of regulation. This might be good for finetuning but may not be very efficient. It is much better to actively regulate a mRNA by direct binding. Also, it will work only if the concerned protein is in limiting ...


1

This is an important topic in immunology, especially for vaccine development. MHC or HLA is a molecule expressed by some cells of the immune system which acts like a "catcher's mitt" and "presents" short snippets of protein to other immune cells. Other molecules act alongside MHC to provide co-signals which promote or suppress immune attack against the ...


1

Patch clamping Or Ultracentrifugation to get soluble fractions. If you get enough of it and u have validated its purity you could runs conductivity probe into your fraction


1

I would say theoretically yes, if you know lots of other information, but practically no, because of all the unknowns. According to this paper cysteine content and therefore sulfur content of proteins vary depending on what kind of organism your organic material is coming from. Animals have more, bacteria tend to have less. The sulfur content as a fraction ...



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