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Single channel refers to the system used to detect the labelled probes. A single channel array, as shown in this slidedeck (slides 6-8) uses one type of molecule for detection. In the example, (slide 8) the optical label is phycoerythrin. Contrast that to a double channel array (e.g slide 7) that uses a "green" or "red" molecule used to label probes. ...


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What is difference between plastid, chloroplast and mitochondira? Plastid is a general term for an organelle which consists of Chloroplasts, Chromoplasts, Leukoplasts/Amyloplasts and Apicoplasts. Chloroplasts (Chloro = green) are involved in photosynthesis; they express Chlorophylls and have the thylakoid structures which are involved in the electron ...


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Edit: C# Functions used in our system, slightly paired down for to simplfy using Microsoft.VisualBasic; using System; using System.Collections; using System.Collections.Generic; using System.Data; using System.Diagnostics; using System.Web; using System.Net; using System.IO; using System.Text.RegularExpressions; public class KEGGCaller { public object ...


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I don't have a definitive answer, but I can perhaps offer some insight. Given the necessary function of rpoA, I would be willing to bet that SigA is the factor responsible for its transcription, so I will focus my discussion there. Predicting promoters without experimentation can be very challenging given their immense variability. The idealized core ...


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This kind of thing is normally calculated using the $K_{a}/K_{s}$ ratio, the ratio of synonymous to non-synonymous substitutions. It is not enough to count mutations for coding sequences, you should also take into account whether or not that mutation will actually change the resulting product. There are various online tools that can help you do this, for ...


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I don't know if this works out for glycans but this tool converts IUPAC name to SMILES format. SMILES is a text based structure notation which can be converted to graphical structure. You can also check these out: http://www.openmolecules.org/name2structure http://www.iupac.org/home/publications/e-resources/inchi.html I am still not sure what you are ...


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Like Liam said, this sort of analysis is dependent on the database you use, which may not be adequate for what you are trying to accomplish. And as far as I know, these databases are at the gene, not isoform, level. So if a change in two conditions is a result of a change in which isoform is expressed, rather than the amount of expression, you will not ...


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blast2go is on the verge of getting commercialized (as they have started selling PRO versions) and my previous experience was not so good with it. I used IntrProScan to associate GO terms with the transcripts, it ran long but it reported all the possible sequence features. To use these custom annotation was tricky for visualization, but thanks to BiNGO, I ...



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