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10

Some that just come to mind, in random order: One cannot skip reading: Richard Dawkins - The selfish gene And, obviously: Charles Darwin - The Origin of Species And, for those interested in the evolution of the brain (and its quirks): David J Linden - The Accidental Mind Oliver Sacks - The Man Who Mistook His Wife for a Hat Not very ...


7

ATryn is a human antithrombin produced in the milk of transgenic goats by GTC Biotherapeutics. It has FDA approval and I believe that it is available for prescription in the USA. Added later, after the emphasis of the question changed somewhat. Proteins produced in a mammalian system are more likely to have post-translational modifications that are much ...


7

A good recollection of the early days of micro and molecular biology is "The Eighth day of Creation" It covers the early use of e. coli, the discovery of phage, transcriptional elements and the impact that DNA structure had. It's very comprehensive and really useful if you are doing molecular biology today.


6

The rationale for the choice of higher organisms as the producing source is based on costs and biological activity. Biological activity. In their active forms, various proteins have post-translational modifications (i.e. glycosylation) which are difficult to reproduce in bacteria. Alan's answer is already exhaustive. Costs. Mammalian cell lines are easier ...


6

You'd think if such devices were used in humans, they wouldn't require a change of lifestyle. They don't say the power always decreases over time: Katz’s snails, for example, produced up to 7.45 microwatts, but after 45 minutes, that power had decreased by 80%. To draw continuous power, Katz’s team had to ramp down the power they extracted to 0.16 ...


5

It doesn't have very many reviews, but The Epic History of Biology sounds like it's perfect. Flipping through the first chapter in the preview, it doesn't seem overly technical in any way, so secondary school-level knowledge is probably enough. If your associates have absolutely no biology experience, perhaps a run through a popular press book would ...


4

Yes, bacteria will produce human (or any organism's) proteins if you introduce their genetic material but there are a few things to consider. First, the introns must be removed from the human genetic sequence. Bacteria do not have the machinery to splice out introns after transcription. This is typically done by using a viral protein to reverse transcribe ...


4

A fantastic book that covers the evolution of modern science since the Renaissance (including a great deal of biology) is The Scientists by John Gribbin. I found that by focusing on the people doing the science in the context of the society in which they lived, I got a much better understanding for why early scientists thought the way they did and researched ...


4

Would this application in humans require that a patient eats more and "breathes more" than average? From the cellular standview, each glucose and O2 molecule that you substract to the cell for use in a second futile cycle, should be replaced. However, I doubt that the amount of glucose/O2 required per hour will be so important to require additional ...


3

By far the best book I've read on the history of biology is A Guinea Pig's History of Biology, by Jim Endersby. It tells the history of the field by focusing on experimental organisms and the contributions which were made by studying them. It has an engaging narrative style and the idea of focussing on organisms' stories is an excellent and original one. ...


3

Total solids (TS): The part of the sludge that remain after drying at 105C for 20 hours:((weight; dried at 105C)/(weight; wet))*100 = TS (%) Volatile solids (VS): The part of the sludge that is combusted at 550C after 2 hours: ((weight; dried at 105C - weight; dried at 550C)/(weight; dried at 105C))*100 = VS (% of TS)


3

I just came across Understanding Biotechnology. There is one very positive and one very negative review. I haven't read the book myself, but it looks that it is exactly what I was looking for: the table of content includes topics like small history overview, genetic engineering, gene therapy, pharmacogenomics, etc. It might be even useful for people with ...


2

I don't know very many books that might be referred to as the Grand History of Biology or anything like that. That's...a big topic. Really big. How about some suggestions for good Biology/Medical History books accessible to lay people: And the Band Played On, by Randy Shilts, an account of the beginning of the AIDS epidemic in the U.S. The Great Influenza, ...


2

My two favorite books are Molecular Biology made simple and fun and Biotechnology for Beginners. Both are well written and fun to read. As their names suggest, the former covers the basics of biology and the latter covers the basics of biotechnology.


2

This book, although a little dated, has given me an incredible appreciation of biology that I never gained in school: What is Life? by Erwin Shrodinger I am not a biologist, but I occasionally work on mathematical-biology and have training in physics and theoretical computer science. This book was much more accessible to me that other books on biology. ...


2

In theory it's possible to have an approximation, but not to know with certainty. Identical twins have the same genomes and look very much alike. Whether it can be done in practice depends on how well we can model the relationship between genes and looks and on how much information is necessary for a judge to permit arresting and questioning a suspect. ...


2

For the definition, see Pelle's answer. For the reasons, both are easy and cheap to measure and can tell you a lot about your material if you have similiar mterial to compare. TS is often used to asses howa material can be handled (pumpable, stackable) In agricultural biogas applications for example, a sometimes used assumption is 1g COD = 1,6 g VS In AD, ...


2

Reverse transcriptase, as the name suggests, uses an RNA template to create a DNA transcript (i.e., complement). Once the DNA complement has been made, DNA polymerase is used because it uses a DNA template to produce a DNA complementary strand. In your specific example, HIV contains a positive-stranded, RNA genome. The HIV RT can use either RNA or DNA ...


2

Although the genetic code is almost conserved among organisms there are some issues to take in account, among which: Every organism has its own codon usage, i.e. the differences in frequency of occurrence in synonymous codons in coding DNA (that reflect the composition of tRNA abundances). So, for example, the codons UUU and UUC both encode for ...


2

DNAse-seq is the most obvious choice. It uses the same idea as DNAse footprinting but measures output using high-throughput sequencing, so it can be done on a whole genome in one shot.


2

DNA sequencing, RFLP, IHC, In Situ Hybridization, Southern Blotting. I think the technique you are looking for (what you are talking about), is an Electro Mobility Shift Assay or EMSA


1

NCBI BioSystems help file contains a list of their sources: http://www.ncbi.nlm.nih.gov/Structure/biosystems/docs/biosystems_help.html#SourceDatabases Please specify what you need as stated in the comments as it is almost impossible to give you more (relevant) information then this.


1

Triacylglycerol synthesis in plants is probably highly conserved. The metabolic pathway is here. The Jatropha genome sequence is also available. Given this some effort has been made to figure out how these genes are regulated. This is a microarray study of Jatropha focusing on fatty acid and TAG biosynthesis. This gives some idea of how TAG genes ...


1

The library is 'created' during a ligation reaction: plasmid vector + insert (e.g. cDNA). That DNA is used to transform E. coli, and, after plating out the transformation mixture, single bacterial colonies are obtained. Each colony contains, to a first approximation, a single plasmid species (i.e with a single insert). So each colony represents a clone, in ...


1

After cloning, the recipient cell is converted into embryonic stem cell. When it is a stem cell, it can produce enzyme telomerase itself which a naturally occurring enzyme that maintains telomeres and prevents them from shortening during cell division in cells. So i think there are no problem on shortening of telomeres.


1

Yes, it is. Dolly died of cancer in early years. Length of the telomeres depends on the donor tissue: "In Dolly's study, the animal produced from fetal tissue appeared to have telomere lengths non-distinguishable from normal controls, while the other two, including Dolly, which originated from adult cells, were found to have shorter telomeres." (Xu & ...


1

I haven't read through all of it, but from the second edition, which I found available online here, it seems to me that it might be a good introduction, but (as expected from the publishing date) it is really missing out on all the next generation sequencing stuff. Which is important, not only because it is my favourite field. I am not sure what you want to ...


1

I found this glossary of Biotechnology and Genetic Engineering off Wikipedia, what do you think? Or The Facts On File DICTIONARY of BIOTECHNOLOGY and GENETIC ENGINEERING What concepts are you necessarily hoping to include? I'm hoping someone actually experienced in this field can advise.


1

I've done experiments with yeast, and they can easily ferment glucose at the rate of 4g/L/d. Probably faster, if one were to optimize it. So your numbers don't seem crazy.


1

Try this. cadnano simplifies and enhances the process of designing three-dimensional DNA origami nanostructures. Through its user-friendly 2D and 3D interfaces it accelerates the creation of arbitrary designs. The embedded rules within cadnano paired with the finite element analysis performed by cando, provide relative certainty of the stability of the ...



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