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There is at least one known case where both the DNA and RNA versions of the same aptamer sequence bind the same target (Lauhon and Szostak, 1995). But you can't generalize this, there are two differences in structure between RNA and DNA, the Uracil/Thymine change and the missing 2'-OH in DNA. Those differences can affect the binding and overall structure of ...


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Aptamers are single-stranded sequences that bind to proteins or other molecular partners. Obviously, ssDNA and ssRNA have very different (in general) binding partners. E.g. DNA is interacting with transcription factors, RNA polymerase, histones etc, while RNA is interacting with ribosomes, reverse transcriptase and others. So, shortly, it is more likely ...


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Sure that will work, or gel purify the ss cDNA, or treat the rxn with alkali to degrade RNA. The real questions in my mind are: What will you use as a primer for the RT? What are you going to do with this pure ss cDNA? If you already have the target cloned (how else would you transcribe it in vitro?), then why not just do a linear PCR where you cut the ...


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I think one of the orginal reasons for using biolistic on plant cells was that it seemed to be the only method which proved to be effective. This is due to some reasons: A wide range of highly successful methodes for prokaryotic cells (calcium chlorid method, electroporation) The plant cell has in comparison to animal cells a additional layer (= cell ...



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