Tag Info

New answers tagged

2

As @canadianer pointed out in the comments section flanking sequence is a sequence that is immediately near the target on either side. Since chimps and humans are closely related and the sequence different is minimal (according to this paper its approx 4% for the entire genome) so using human primers is totally fine. Now if take a look at the picture of ...


4

Yeast can produce up to 16-17 v/v% ethanol without dying according to this article. Yeast is a primary industrial ethanol producer, it produces ethanol even under aerobic conditions, in contrast to bacteria that usually ferment sugar to ethanol under anaerobic conditions. Bacteria can also withstand up to several percent v/v concentration ethanol in their ...


4

This article gives an excellent review on yogurt manufacturing, but to summarize: -Raw milk goes through centrifugation to remove somatic cells and other solid impurities. -Thermalization is conducted at "60–69 °C for 20–30 s, aiming at the killing of many vegetative microorganisms and the partial inactivation of some enzymes." After this point, the milk ...


-1

As when air tight seal is opened there is a exposure to air and Lactic acid in yogurt in the presence of oxygen converted into Acetic Acid which is vinegar and produce sour taste.


0

Commercial yoghurt usually contains alive Lactobacillus, as exemplified by the fact that you can use them to "seed" your yoghurt at home. I actually don't think they add anything. In France at least it is just pasteurized milk and lactobacillus (ex from Auchan yogurt list of ingredients: Semi-skimmed milk, lactose and milk protein, lactic ferments). Some ...


2

Oligos from well-known companies are pretty good to my knowledge. Problems begin after 100-300bp oligos/dsDNA fragments. What I suggest is PCRing your vector like that: Primer F: ---------------------__________ vector: ======|v site for 36 bp insert v|==========... Upstream Downstream ______----------------- ...



Top 50 recent answers are included