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http://www.bbc.com/news/health-35459054?SThisFB%3FSThisFB Take a look at this article above. It had come out in BBC sometime early this year. Gene editing is a crucial part of cloning. Cloning is a way of artificially creating cells in a laboratory. CRISPR-Cas9 is a currently very new, but a trending technology for editing the genome in modern healthcare. ...


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As you already wrote there are two basic ideas: Synthesis of the desired gene sequence and use of existing DNA. DNA Synthesis is getting cheaper so it is becoming the method of choice for generating DNA-samples up to about 5000 bp (for example see the IDT-page for commercial gene synthesis). If this is too short for your desired sequence there is the ...


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I'd like to vouch for adoptive cell transfer therapy, ACT, using tumor-infiltrating lymphocytes, TIL (Lee & Margolin, 2012). While the therapy is currently geared largely for metastatic melanoma, there are investigative studies involving TIL ACT in other cancers such as head/neck, cervical, ovarian, breast, etc. I want to note immediately that this is ...


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There is no general therapy for cancer - as there is no general cancer. Cancer is the umbrella term for a disease where you have body cells growing without control, leading eventually to death. But since there is a wide variety of cells in the body, there is also a very wide variety of different cancers. To treat these, surgery is often the first line of ...


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Both TALEN and ZFN gene editing technologies use a pair of single-strand endonucleases that ultimately lead to a double strand break. Cas9 in CRISPR-Cas technology, however, cuts both the DNA strands (there are some Cas enzymes in certain bacterial species that cut only a single strand [ref]).       Image courtesy: ...



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