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Injecting into a fresh organ seems like a needless complication if you're just validating your antibody and not interested in gathering data about the interaction between the cells and the organ. (In fact, if you're using anything more complex than a flatworm I'd hope your IRB would have problems with needlessly sacrificing animals to get those organs.) It ...


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I don't think that there is a one-size-fits-all answer to this question. There are different types of arrest and there might be different triggers depending on the epithelial cell type. Even in the cited Hammond et al paper, note that the effect of serum is not an immediate arrest. Human mammary epithelial cells (HMEC) could already go through 3 to 4 ...


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Work with vectors expressing oncogenes or knocking down tumor suppressor genes (particularly if vectors are based on lentivirus) is often done under "BSL-2+" conditions. This essentially means that personal protective measures required for BSL-3 are used, but the specialized ventilation systems and so forth of BSL-3 physical laboratory space are not ...


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Maybe, cells should be contained under BSL-2 and agents (viral particles etc) under stricter BSL-3? That is, after immortalization you can keep cells under lower security. Biosafety Level 2 builds upon BSL-1. BSL-2 is suitable for work involving agents that pose moderate hazards to personnel and the environment. Biosafety Level 3 is applicable to ...



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