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Supply of cells growing in adherent culture is unproblematic, as long as you don't fill too much media in the petri dish/cell culture flask. Oxygen supply here work by diffusion which is getting more problematic if the layer of media covering the cells gets to thick. I usually follow the recommendations of Corning for the amount of media to be used in the ...


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In most cases, it's not a good idea to replace glucose with glycerol in animal cell media. Animals do possess the ability to metabolize glycerol, via a pathway starting with the enzyme glycerol kinase. However, glycerol kinase is only expressed in certain cell types, such as liver cells and kidney cells. References: ...



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