New answers tagged cell-culture
I realize this is an older topic, but I'd like to clarify some misconceptions: 1) DAPI is usually referred to as a semi membrane-permanent dye because its penetration through viable cell membranes is concentration dependant. At lower concentrations it is mostly excluded from viable cells, while at higher concentrations it stains live and dead cells ...
I concur with @Bez but wish to elaborate on the meaning of 'conserved'. It is generally used in the context of evolution. A conserved characteristic or gene or protein means that it has 'survived' a long time without being altered. As @Bez mentions, certain parts of the protein machinery in eukaryotes is very different from prokaryotes, the latter being ...
OD = Optical Density As you grow cells in broth, the broth becomes cloudy. The more cells there are, the cloudier it is. After you grow your cells for a while, you can measure their progress by shining a light through your sample. The more light your cell culture absorbs (higher absorbance is equivalent to higher OD) the more cells there are in it.
You should check the composition of the coating by using trypsin, e.g. poly-L-lysine or poly-D-lysine are usually applied: Polymers of both D- and L-lysine are used to coat solid surfaces. Poly-L-lysine has been reported to improve the protein coating of ELISA plates. 6,7 However, in culture applications, certain cells can digest poly-L-lysine. In ...
Generally the coating is of poly D-Lysine. Proteases act only on L-amino acids. So, I guess nothing should happen to the usual coated flasks. I have reused the flask even after 2 rounds of trypsinization, and the cells seemed to be fine. In your case, the matrix is made of proteins isolated from animal sources. They are susceptible to proteolysis. You are ...
Perhaps you're looking for these hanging inserts, for example: "Millicells"
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