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OD = Optical Density As you grow cells in broth, the broth becomes cloudy. The more cells there are, the cloudier it is. After you grow your cells for a while, you can measure their progress by shining a light through your sample. The more light your cell culture absorbs (higher absorbance is equivalent to higher OD) the more cells there are in it.


You should check the composition of the coating by using trypsin, e.g. poly-L-lysine or poly-D-lysine are usually applied: Polymers of both D- and L-lysine are used to coat solid surfaces. Poly-L-lysine has been reported to improve the protein coating of ELISA plates. 6,7 However, in culture applications, certain cells can digest poly-L-lysine. In ...


Generally the coating is of poly D-Lysine. Proteases act only on L-amino acids. So, I guess nothing should happen to the usual coated flasks. I have reused the flask even after 2 rounds of trypsinization, and the cells seemed to be fine. In your case, the matrix is made of proteins isolated from animal sources. They are susceptible to proteolysis. You are ...


Perhaps you're looking for these hanging inserts, for example: "Millicells"


Shear stress $\tau$ in this small sizes is usually measured in dyne/cm2 or N/m2 = Pa. The equations betweeen them: $1dyn/cm^2 = 10^{-5}N/cm^2 = 0.1N/m^2 = 0.1Pa$. What kind of damages zygotes can suffer by pipetting? Using scanning electron microscopy, we found open holes on the surface of lysed eggs, indicating failure of the plasma membrane to ...


Clones are genetically identical daughter cells from one parent cell. So when you start you culture from one cell, all new cells are clones of this cell. The first three cells (red, green and blue) undergo clonal expansion. If you multiply cells in cell culture you usually start with thousands of cells, as this can be seen in the second part of the ...

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