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1

I can't tell if you're asking about glassware or work surfaces (hoods, benches etc), but... We use regular old dawn dish soap in my lab because what's more important than the soap you use to wash you're glassware is the water you use to rinse it. We teach our undergrads the 3-rinse-rule. After soaping, every item gets 3 rinses with normal tapwater (or ...


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Bacteria or other microorganisms cannot really grow on anhydrous (totally dry) glucose because they need water. However, they can remain there and cause contamination. Even if you haven't actually touched the glucose sample, there are many bacteria suspended in the air and they may settle down in your glucose packet. When you take the glucose out for ...


2

Supply of cells growing in adherent culture is unproblematic, as long as you don't fill too much media in the petri dish/cell culture flask. Oxygen supply here work by diffusion which is getting more problematic if the layer of media covering the cells gets to thick. I usually follow the recommendations of Corning for the amount of media to be used in the ...


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In most cases, it's not a good idea to replace glucose with glycerol in animal cell media. Animals do possess the ability to metabolize glycerol, via a pathway starting with the enzyme glycerol kinase. However, glycerol kinase is only expressed in certain cell types, such as liver cells and kidney cells. References: ...



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