Tag Info

New answers tagged

0

You're both correct. Turbidity is causes by excessive light reflection events from all of the particles, and is actually how it is measured in a sample. As to cells, they both both reflect light that is incident on a membrane, but they will also refract light that does manage to enter the cell because of the difference in refractive indices. A portion of the ...


3

For assaying effect of alcohol on cell growth:     Prokaryotic Cells Take ~5ml medium (LB for E.coli) in test-tubes/plastic tubes and add appropriate concentration of alcohol(s) in these. Inoculate 1% bacteria from a starter culture (OD~0.6) After different time intervals or a fixed time point take some culture, dilute and spread ...


0

I am not sure about what they used in 1958 (perhaps Sorvall). However you may look at recent papers for ECM protein isolation (if that was your objective). Have a look at this article. From materials and methods: ECM protein isolation. Fibroblasts or A431 cells were removed from the surface of the culture plate with 2 mM EDTA in PBS for 3–5 min ...


4

Based on T Abraham's answer, a hemocytometer would work. However, a hemocytometer requires a microscope, but if you are in a cell lab, you probably have access to a microscope. I would recommend using mammalian cells instead of bacteria, they're larger and easier to see, and more relevant to human health. If you're interested in liver toxicity in particular, ...


4

Great idea! It seems that this research has been done before and your hypothesis is correct, but testing a wide variety of alcohols and testing your hypothesis for each one of them would be a great project. Now, to do this, I would expect you would need to check the growth of the cells after certain periods of time. How would you do this? Well, the best ...


2

This company sells a vacuum manifold for 24, 48 and 96 well plate formats.


9

The cells never died in the sense that they kept replicating, individual cells still died. They were safely cultured in petri dishes before Henrietta Lacks died. The cells came from a tumor that developed from her cervix. The cervical cancer cells had developed high telomerase activity. Telomerase builds telomeres on the ends of DNA, protecting the ...



Top 50 recent answers are included