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MitoSOX looks pretty cool. I've never used it myself, but I'm sure you could see some cool mitochondrial dynamics going on. Its a bit pricey though, but all dyes are going to be. Even at only 8 hours you should probably be able to see a few divisions, which would be cool with hoechst.


4

Some thoughts on this. First of all, the positive-inside rule, proposed by Gunnar von Heijne, is an empirical rule based upon observations, not one derived from theoretical considerations, so any explanation is simply an attempt at a rationalisation. Having said that, here are three of those rationalisations: the membrane potential is usually negative ...



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