New answers tagged

1

Supply of cells growing in adherent culture is unproblematic, as long as you don't fill too much media in the petri dish/cell culture flask. Oxygen supply here work by diffusion which is getting more problematic if the layer of media covering the cells gets to thick. I usually follow the recommendations of Corning for the amount of media to be used in the ...


1

The solid precipitate does not contribute as much to the pressure inside the vial as the gas does. Intuitively, the gaseous molecule contributes to the pressure by collisions that exert force on the vessel (which would push the water out). By contrast, the solid precipitate only contributes to the pressure insofar as it decreases the volume of the vessel, ...


1

This is an interesting question (I really mean this — see below), for which a straight answer is remarkably difficult to find on the web. When I googled for it I got pages with statements that obligate anaerobic bacteria still had the electron transport chain (ETC) and ATP synthase as there were different electron acceptors other than oxygen. Yes, we know ...


2

The good ol'electron transport chain (ETC). Before beginning let us begin by looking at the structure of a mitochondrion and the purpose of the ETC. Take of note four things: The intermembrane space The matrix The electron transport chain proteins and ATP Synthase Now lets talk about the purpose of the ETC, essentially its job is to create a H+ ...


0

In addition to the long and complete answer above, I want to point out something extra that is missing in the explanation: The proton gradient works both ways (like all gradients). Lowering the external pH is not the only way to create a gradient, increasing the internal pH also works perfectly fine.


3

Diffusion, is by definition (Ficks Law) describing movement along or against some gradient (here its concentration) even if the mechanism of transport differ: active, passive, facilitated. The setup of the electrochemical gradient across membrane, like any thermodynamic process is not perfect, there are always entropy losses. In this case, this manifests as ...



Top 50 recent answers are included