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You are right in thinking mitochondria are needed to use oxygen (for respiration) — but only in eukaryotes. They are not needed in bacteria because… bacteria are mitochondria! Well that’s not true — it’s mitochondria that are bacteria. And that’s not true either — mitochondria were bacteria. Apologies for the theatricality, but all this is to emphasize the ...


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Supply of cells growing in adherent culture is unproblematic, as long as you don't fill too much media in the petri dish/cell culture flask. Oxygen supply here work by diffusion which is getting more problematic if the layer of media covering the cells gets to thick. I usually follow the recommendations of Corning for the amount of media to be used in the ...



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