New answers tagged cloning
check out the new gibson resource on the Addgene.com website https://www.addgene.org/plasmid_protocols/gibson_assembly/ Addgene has a lot of technical resources and adds more every day May also already have the plasmid you need.
To learn about the process of how cloning works, you need to appreciate the underlying principles. A general bio textbook should suffice (most popular one is Alberts, where you should focus on chapter 21, but maybe read other chapters for background) Nature Scitable is also a good source. I'd discourage reading research papers at this stage, primary ...
Here is a good overview of primer design considerations when using Gibson assembly. http://j5.jbei.org/j5manual/pages/22.html
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