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Yes. In principle you will have a vastly increased ability to customize the boundaries of each protein domain with a primer and PCR based approach. It is not obvious to me that the Gateway Cloning System is required, or necessarily the best choice for this approach, but that is likely subjective. Are you familiar with, or aware of, the efforts of the SGC ...


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Human female cells contain most of the genetic information required to make a male, but they do not contain a critical component: The Y chromosome. This is a relatively small chromosome. Wikipedia claims we have identified around 200 genes on it to date, compared to estimates of 20,000 - 25,000 genes overall in the human genome. Importantly for your ...



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