New answers tagged cloning
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check out the new gibson resource on the Addgene.com website
https://www.addgene.org/plasmid_protocols/gibson_assembly/
Addgene has a lot of technical resources and adds more every day May also already have the plasmid you need.
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To learn about the process of how cloning works, you need to appreciate the underlying principles. A general bio textbook should suffice (most popular one is Alberts, where you should focus on chapter 21, but maybe read other chapters for background)
Nature Scitable is also a good source.
I'd discourage reading research papers at this stage, primary ...
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Here is a good overview of primer design considerations when using Gibson assembly.
http://j5.jbei.org/j5manual/pages/22.html
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