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usually the growth of bacteria is slowed down as they are in freezing temperature and they almost carry out no metabolic reaction at this stage.but another problem occurs that is ice crystals formation which disrupts or pierce the bacterial cell wall causing the death of bacterial cells,this problem can be solved by adding glycerol or any other ...


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Yes, they do. For a look at survival of Lactobacillus and other bacterial species after multiple freeze/thaw cycles, check out Harrison 1955 (below). The awesome hand-drawn graphs show that many bacteria survive after being frozen for 11 weeks. There's also a figure on the following page showing that many bacteria also survive after multiple freeze-thaw ...



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