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We had to do this frequently. What I would first recognize is that all of your current methods only work for at the small scale and that for you to purify mg quantities of DNA, you need to work with mg scale equipment. First of all, you should examine your "upstream" process. Are you doing your PCR at scale? Typically, we have scaled out by going with ...


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The sodium ions will bind to and neutralize the (-) charge on the phospho backbone of DNA. This reduces hydrogen bonding sites for water, which then results in less solubility. Edited for clarity and because I didn't answer the question the first time. Sorry about that. The extraction of DNA is from the interphase layer, which is a very small fraction of ...



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