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As pointed out by aandreev, the greater the size of the insert relative to the flanks lower will be the recombination rate. This is because the donor DNA essentially becomes non-homologous. See the figures below:                 Figure1: Effect of insert size on recombination ...


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In my view there is direct link between probability of recombination and size of the insert. Smaller inserts with larger flanks get incorporated easier. That I understand from reviewing studies based on Cas9/CRISPRs system. Same should stand for HR-mutagenesis in yeast. So, short answer will be: shorter inserts are more efficient. However, I couldn't find ...



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