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7

Note: In your PCR program you always set extension time. Case: Product length = 500bp PCR extension time = 50sec Assuming that polymerase adds 1000 nt/min Cycle 1: Strand that binds FP: extends ~800nt to the right (as per the polymerization rate): 300 bp ahead of RP complementary site. This product is lets say P1 Strand that binds RP: extends ~800nt ...


5

So in mitosis, the cell has to split itself into two cells; each daughter cell has a functional genome that may again split into more daughter cells. The cell replicates the DNA before dividing, so the error in replicating 3x or 4x is that upon division, the daughter cells will have more DNA than the initial cell, and every generation will have more DNA than ...


4

Summary: In bacteria or organisms with only one well defined replication origin and a circular chromosome, yes for a given DNA region the same strand is replicated discontinuously. In high order animals, which replicate chromosomes using several origins of replication (ori), this is less clear as the way ori are recognized is still not fully understood but ...


4

I'm not completely clear when you say "what makes the replication terminate when the polymerase reaches the primer at the other end" since when you perform a PCR you go through three phases. The denaturation, whereby the two DNA strands become single stranded, then the annealing, which is when primers attach to their appropriate matching site (but the ...


3

TCT AAC TGA TTA GC T CTA ACT GAT TAG C TC TAA CTG ATT AGC AGA TTG ACT AAT CG <<< this is the ORF A GAT TGA CTA ATC G AG ATT GAC TAA TCG If the sequence comes from the middle of a gene we assume it should encode an open reading frame. For this sequence only 1/6 frames does not include a stop codon (shown above in italics). So in standard ...


2

For DNA replication and transcription you need NTPs. In a dsDNA purine content will be same as pyrimidine content. I am considering that all nucleotides are synthesized de novo which would consume more ATP than getting nucleotides from the salvage pathway. ...


2

On the sequence alone, you can answer neither of these questions because: from the sequence alone you don't know anything about the gene or the promoter. the same is true for the orientation and the codons, since you don't know if the code is in frame or not. If one base is cut-off from the original sequence, your codons shift, and don't show the original ...


1

The immune system recognizes patterns - incase of innate immunity and shapes - incase of active immunity. I am making a few assumptions: The Shrinking would result in a smaller version of "the person" - meaning he/she is a bacteria sized person capable of executing all actions that a human can do. The shrunk person is placed inside the blood vessel or ...


1

This is a very interesting question. As others have alluded to, the body generally recognises self and non-self. However, as with any biology it's not that simple. Even leaving aside autoimmune disorders, the body doesn't always attack non-self and doesn't always leave self alone. Think of a pregnancy - the foetus is non-self, and yet the body doesn't reject ...



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