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Note: In your PCR program you always set extension time. Case: Product length = 500bp PCR extension time = 50sec Assuming that polymerase adds 1000 nt/min Cycle 1: Strand that binds FP: extends ~800nt to the right (as per the polymerization rate): 300 bp ahead of RP complementary site. This product is lets say P1 Strand that binds RP: extends ~800nt ...


4

I'm not completely clear when you say "what makes the replication terminate when the polymerase reaches the primer at the other end" since when you perform a PCR you go through three phases. The denaturation, whereby the two DNA strands become single stranded, then the annealing, which is when primers attach to their appropriate matching site (but the ...


3

TCT AAC TGA TTA GC T CTA ACT GAT TAG C TC TAA CTG ATT AGC AGA TTG ACT AAT CG <<< this is the ORF A GAT TGA CTA ATC G AG ATT GAC TAA TCG If the sequence comes from the middle of a gene we assume it should encode an open reading frame. For this sequence only 1/6 frames does not include a stop codon (shown above in italics). So in standard ...


2

On the sequence alone, you can answer neither of these questions because: from the sequence alone you don't know anything about the gene or the promoter. the same is true for the orientation and the codons, since you don't know if the code is in frame or not. If one base is cut-off from the original sequence, your codons shift, and don't show the original ...



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