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TCT AAC TGA TTA GC T CTA ACT GAT TAG C TC TAA CTG ATT AGC AGA TTG ACT AAT CG <<< this is the ORF A GAT TGA CTA ATC G AG ATT GAC TAA TCG If the sequence comes from the middle of a gene we assume it should encode an open reading frame. For this sequence only 1/6 frames does not include a stop codon (shown above in italics). So in standard ...


On the sequence alone, you can answer neither of these questions because: from the sequence alone you don't know anything about the gene or the promoter. the same is true for the orientation and the codons, since you don't know if the code is in frame or not. If one base is cut-off from the original sequence, your codons shift, and don't show the original ...

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