New answers tagged dna-replication
I think the following should happen: There would be no amplification but we will get n copies of single stranded DNA after n cycles and the only double stranded DNA with started with but with a new complementary strand.
Using 1 primer will results in amplifying the original sequence in each cycle in a linear fashion: the primer will guide a single run of the polymerase. In opposed to the "chain reaction" in PCR which is exponential. Therefore, with one primer you linear amplification of say x40 times the original number of molecules you started with, whie with two primers ...
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