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Small RNA sequencing is good for a preliminary screen because as with most high throughput experiments, the sample size is less and therefore inter-sample comparisons may not be accurate. For miRNA expression calculation, I use mirdeep2's quantifier script with a slight modification. The script basically aligns the reads to known pre-miRNAs and finds if ...


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I would be very careful in making such a claim from sequencing data. There are well known biases in sequencing due to things like GC composition and hexamer composition. Because of this, it's possible to compare relative levels of some species across treatments but tricky to do so within a given sample or samples. One way around this is to perform qPCR using ...


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First of all: Honey is not a byproduct of bees - it's their main and most important product which stores energy for the bee hive for the time when no flowers are available. Honey itself consists (according to the USDA database) to about 99.5% of water and sugars (82.5% sugars, 17% water). The sugars are mostly fructose and glucose, but also some other mono- ...


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Enter it in BLASTX. This will give you any protein sequence matches as well as likely homologues given a nucleotide sequence.


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You might want to try searching against NR/NT database using BLAST. This way you will get to know what this sequence might be similar too. The length of the sequence is too short to code for any meaningful protein.



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