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The largest contributor to sequencing coverage (on a Hiseq, I assume) is how many reads you get, which mostly depends on what percentage of a flow cell you give it. Obviously the rest that you mentioned contributes, and purity might be a big issue if you are looking for a subset of cells you were trying to isolate out of a larger pool, but the easiest way ...


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Sequencing does not seem necessary for this... most plasmids contain an antibiotic resistance gene (ex. kanamycin-resistance) that will allow cells that have been appropriately transformed to survive in the presence of kanamycin. If it's just a PCR product, Sanger sequencing seems the best way to go. It is quite specific and not prone to algorithmic errors ...


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We will be needing some additional information to answer your question more completely: What sequencing platform are you using? Illumina HiSeq/MySeq; Ion proton What type of sequencing (as noted by @CMosychuk): Exome or Whole Genome? What type of variant caller (as noted by @CMosychuk): Unified Genotyper; Haplotype Caller; TVC The QUAL metric is heavily ...


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I'll take a stab at this question though some of the comments might have already answered what you're looking for. A human reference genome was created to create a framework through which we can study the human genome. Every human will have differences from this reference and something you'll even find difference within the same person between cells. Using ...


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I think there are two concepts that you need to understand to understand what "sequence the human genome" mean; within vs between species variation and genome consensus Within vs between species variation There is indeed variation in the human population. We are not clones of each other. One can for example consider the average number of pairwise ...



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