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So, your blank is everything but the enzyme? That is, substrate and DNSA with 100 uL water? If yes, measure, as negative controls, substrate alone and DNSA alone. If one of them is also highly absorbing light, it may be contaminated, and a new vial should be ordered. If both are highly absorbing, it may be the water you are using, or the spectrophotometer. ...


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According to this instruction it can be done for 1 hour but this is for large plasmids. I agree with Chris that it should not be of a problem. Alternatively you can setup your 37°C DpnI incubation step in a PCR machine and after however long that is recommended, you can set the PCR machine to cool to 4°C automatically, that way you can leave it there for as ...


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This might be a good article to read: http://www.ncbi.nlm.nih.gov/pubmed/24497635 Robert Kerns's lab has been doing a lot of work on fluoroquinolines, so look into his other work as an entry to the literature. And apparently X-ray crystals have been done for at least parts of this complex. There is some evidence for fluoroquinolines binding to DNA, then ...



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