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If you did enough replications in your experiment, you could harvest one or more plants and measure fresh weight, and then gently oven dry for about 48 hours at like 160 degrees F and then take a dry weight as a measure of biomass. I don't know a non-destructive way to do it. A lesser alternative would be to measure plant height and number of leaves, though ...


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Not sure if this question is still valid, but it was marked as unanswered and frequently viewed, so here's a go :) These days most polymerases (certainly for Real Time PCR) are so-called Hot Start enzymes (ask your manufacturer, because they save you a whole lot of trouble!). They are modified with a protein that prevents them from working until activated ...



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