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Necrotic cells could be trypan blue positive. WST-1 Assay could reflect cell number by measuring mitochondrial dehydrogenase, therefore, you could not know how many cells die in dishes. When you define apoptosis in your experiment as caspase dependent cell death, measuring caspase activity is the best. PS Trypan Blue Staining actually is a method to see ...


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It seems you are confused with some other gene instead of caspase 9, but if KO of some gene causes development of breast cancer, and you want to compare something between none-cancerous and cancerous states, you could take several ways: See if you could have littermates carrying and not carrying cancer. You could compare them. Sometimes, phenotype do not ...


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The short answer is that it is likely a bad experiment; being that it would be expensive and is not likely to provide useful new information. The long answer (i.e. why) is identifying an interesting target gene (e.g. caspase-9 in breast cancer) is only the first step, and considerable study of what is already known is required before planning an experiment ...



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