New answers tagged experimental-design
1
Here is a good overview of primer design considerations when using Gibson assembly.
http://j5.jbei.org/j5manual/pages/22.html
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Disclaimer: I haven't ever done a Gibson assembly, but here is my theoretical understanding of how to design your primers. You need four 40mers each consisting of 20 bp segments derived from the vector and the insert and corresponding to the junctions that you are trying to create. In the diagram below the dotted lines represent the junctions between the two ...
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Supplementary information from the Comments from the first answer:
I have perhaps found @terdon's reference? Not sure if this is it, but by measuring transcription events in individual cells, Taniguchi et al. certainly make the case that there is no correlation between protein and mRNA levels.
However microarray data (and most rnaSeq and qPCR ...
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The technique described here is called microarray. Your question has given me an opportunity to put forth one of my opinions about certain problems of gene expression studies.
Gene expression is a measure of the activity of any gene. If the gene performs its activity in the form of a protein, then its expression should be a measure of the protein. If a gene ...
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