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Sounds like you should look into Bioconductor's flow suite. I actually really like doing flow analysis in R, but it takes some practice and getting used to. The biggest problem you will run into (in my opinion) is gating. It's not easy to setup a GUI point and click gate. Rather, you'll be more forced and inclined to set limits with algorithms or hard ...


2

I realize this is an older topic, but I'd like to clarify some misconceptions: 1) DAPI is usually referred to as a semi membrane-permanent dye because its penetration through viable cell membranes is concentration dependant. At lower concentrations it is mostly excluded from viable cells, while at higher concentrations it stains live and dead cells ...



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