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If you can see sample DNA, but not your DNA weight marker than the problem is in the marker. If it has been used a few times it may well be contaminated and degraded. I would suggest to check your marker and run a gel with your old marker and with a new freshly opened/prepared batch of marker DNA. Take care not to cross-contaminate the vials. If the new ...


Possibly You don't mix ladder containing microtube by Sampler. You should slowly Fill and emptying Sampler for several times when pick it for use, mix it well, but do it without creating a bubble.one more thing, check your ladder by "NanoDrop"


As you can see from the button of the gel, the brightness of the bands have reduced even for the DNA ladder/marker. The main reason for this is that when you are running an agarose gel containing EtBr, due to the positive charge of the EtBr, the stain runs to the negative chamber, against the direction of the DNA migration as @Chris has pointed out! Although ...

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