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A few years ago plant scientists introduced a protein (afa3)in tomato from Winter Flounder, a type of fish surviving freezing conditions. The idea was to make a frost resistant tomato but I believe they did not have much success. Anti-GMO activists had a field day however with baseless claims of making tomato's smell like fish and other nonesense.


Introductory textbooks will not get into the details of the lac operon. Basically, the operon is expressed constitutively at a low level that means that Beta Galactosidase and Lactose Permease are expressed at low levels by the bacterium. This is because it takes a little bit of time to build up the concentration of LacI in the cell before it can start to ...


One example that I am aware of where an animal transgene was put into a plant was to produce the ZMapp, a chimeric, monoclonal antibody against the Ebola virus. This is a wikipedia article on ZMapp, that gives you an idea of the process. Other than this, I am not all that familiar with plant transgenics, other than Round Up resistance genes that were ...


DNA mutations arise from either replication errors or the effect of mutagens. Cell division can go into what is known as the G0 phase where division is put on hold. This state is also referred to as a quiescent state (see the related genes here), which is distinct from senescent states where damaged cells are prevented from replicating by, for example, the ...


You are trying to express the plasmid in C. elegans, so it should be a worm expression vector and promoter. Otherwise, it will be expressed in the host bacteria, but not in the worm, so you won't get the phenotype you're looking for. One thing you can do is add a bacterial origin of replication to the vector (if one isn't already present) so the bacteria ...


Following the link you provided confirms that the C. elegans expression vector will express your insert of choice in the transgenic animals' pharyngeal muscle cells (driven by the myo-3 promoter). From examining the two images of the plasmid's restriction map on the page you linked to, I infer that a SacI x XbaI double digest should excise the mCherry ...


"pLT63 contains 0.8 kb of the fem-1 gene inserted into the L4440 vector" (1). The gene fem-1 is involved in determining the male sexual trait in C. elegans (you can verify this on uniprot). It may be best, however, to stick to a "normal" plasmid like L4440 that'll just have your tph-1 insert ligated in.

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