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It's a probe to detect external 'Alien' RNA standard, a synthetic mRNA commercialized by Stratagene/Agilent. The Alien RNA transcript is a ~500-nt, polyadenylated RNA molecule that is synthesized by in vitro transcription. The Alien RNA transcript is nonhomologous to all known nucleic acid sequences currently in public databases, as determined ...


2

Supplementary information from the Comments from the first answer: I have perhaps found @terdon's reference? Not sure if this is it, but by measuring transcription events in individual cells, Taniguchi et al. certainly make the case that there is no correlation between protein and mRNA levels. However microarray data (and most rnaSeq and qPCR ...


6

The technique described here is called microarray. Your question has given me an opportunity to put forth one of my opinions about certain problems of gene expression studies. Gene expression is a measure of the activity of any gene. If the gene performs its activity in the form of a protein, then its expression should be a measure of the protein. If a gene ...


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Wikipedia gives a very good explanation of this, on the page for the suprachiasmatic nucleus. For example, in the fruitfly Drosophila, the cellular circadian rhythm in neurons is controlled by two interlocked feedback loops. In the first loop, the bHLH transcription factors clock (CLK) and cycle (CYC) drive the transcription of their own ...



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