New answers tagged

1

I think the presence of a single terminator is related to the high processivity of T7 RNA polymerase. T7 RNA polymerase will transcribe a circular plasmid repeatedly before dissociating. The T7 terminator after ORF2 ensures that the transcript terminates efficiently. Based on your schematic, these are the potential mRNA transcripts from that construct: ...


1

I think a clear way to rephrase this question would be "How beneficial a mutation needs to be to behave really differently from neutral mutations?". I am answering to this question. Neutral mutations and nearly neutral mutations The probability of fixation of a new neutral mutation is $P_{neutral}=\frac{1}{2N}$, where $N$ is the population size. An ...


2

The primary product of protein coding genes are mRNAs. When we talk about measuring gene expression we want to assay the steady-state levels of a specific mRNA within a cell. This is usually accomplished by starting with a large number of cells and harvesting all of the mRNAs from all of the cells. One way to measure the expression level of just one ...


0

According to next generation data: I was never working directly with mRNA but what I got from the bioinformatician was something like this: you extract your mRNA, sequence it, filter it according to the quality & length, assemble it and what you then have is something like "transcripts". Those you are counting: xy of transcript1 & yx of ...


0

My experience is with Affymetrix probes for Drosophila, not H.sapiens, and only with one version. Nevertheless I'll describe the situation I encountered in case it is relevant to yours. Apologies if it is a red herring. What I did with the Affymetrix data sheet was use it to construct my own SQL relational database containing probesetIDs and geneIDs (as ...



Top 50 recent answers are included