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Single-stranded RNA can easily form secondary structures, a very important example of this are tRNAs. From a quick look at the article you read it seems that these Alu repeats work similar to riboswitches in the 5'-UTR. They can form secondary structures that block translation initiation, riboswitches commonly do this by hiding the Shine-Dalgarno sequence ...


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It's because E.coli BL21(DE3) are competent cells. The competent is the key here as the cells were chemically treated so the transfection can be performed by heat-shock with high efficiency. This means these bacteria are quite fragile, due to the chemical treatment, and therefore are very sensitive to both mechanical and thermal shocks. Pre-heating the ...


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Probably, there are narrow and wide definitions for imprinting. I am guessing the history of researches about imprinting would be like the followings. Initially, maternal or paternal phenotype transmissions were recognized and some of such phenotype seemed because the maternal or paternal responsible gene does not work. As you know this is clearly ...



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