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If you're expressing the gene in E. coli, then you're almost certain to be using a plasmid or other ectopic gene vector. There are a wide variety of plasmids available, with many different promoters. With a commercial plasmid, you're not adding in the promoter sequence, but the gene sequence. You pick the promoter you want and then clone your gene sequence ...


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There are several mechanisms by which the expression of a gene can be completely turned off. Certain network architectures can ensure foolproof repression (for e.g. by using multiple repressors parallely or additional epigenetic silencing mechanisms). Bistable switches can also ensure robustness of expression in a way that small fluctuations in the ...


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aI used to work at Affymetrix when most of these arrays were designed. I was not on the design team itself, but I can maybe talk about this a bit more. RNA Array designs were built to cover anything that might possibly be real transcript in the mix of EST collections, cDNA, in silico gene detections and miscellaneous entries in public databases. There ...


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You are correct in saying that Crick, in his Wobble Hypothesis, proposed that “the base on the third position of the codon and that on the anticodon need not be complementary”, but the “need not be” in your statement is a paraphrase of the “some” in Crick’s original statement: “It is suggested that while the standard base pairs may be used rather ...



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