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In the absence of molecular sequencing data the most accurate estimates are based on reassociation kinetics, more commonly known as Cot curves. Here is a classic paper from 1974 that describes the basic approach. While pulsed field electrophoresis can be used to resolve the chromosomes of species with relatively short chromosomes (like baker's yeast), there ...


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Gel electrophoresis is a basic lab method to get size info about nucleotide fragments, although one might need to chop up larger DNA to be able to apply this method. The wikipedia page covers the topic well, thus I'd suggest reading it. I'll just give a short outline: Nucleotide fragments are loaded into a gel (agarose or acrylamide). The gel is put into ...


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I guess you could estimate the length various ways. Pulse field electrophoresis could show DNA length, but you need a standard. When you combine Karyogram and FISH, you would could make a map. By genetical approaches, you could estimate distance on the map by centimorgan. Then you could know how many centimorgan chromosomes are. One centimorgan would be 1 ...


9

There is both an upper and lower limit which are species specific. The upper limit is caused by incomplete segregation of sister chromatids and subsequent trimming of the long arm(s). The reason for the lower limit is, as far as I can tell, unknown. Upper Limit Schubert I, Oud JL. 1997. There is an Upper Limit of Chromosome Size for Normal Development of ...


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Let's try and answer all three parts of your question. Sequencing The general method is the same. Sequencing is just sequencing. But as for every single sequencing, there are factors to consider and protocols to be selected. One important thing is, that you might want comparably long reads to cope with the repeats and the general large size of plant ...



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