Hot answers tagged growth-media
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(Super Optimal broth with Catabolite Repression) SOC media has glucose where as Luria Broth (LB) doesn't. SOB also has a more delicate salt balance.
Naturally the glucose will help the cells recover and grow faster after the heat shock. Your transformation efficiency should improve since less cells will die.
On the other hand, the reason why SOC media ...
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Not sure that I have ever come across trace metals in recipes (I assume that you aren't referring to the Ca/Mg salts component of M9), but it is certainly the case that many K12 strains (e.g. C600, DH5α) require thiamine because they are thi mutants. This doesn't apply to BL21 since it is from a different lineage (it is a B strain). In a lab where ...
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I have used terrific broth to grow up transformed E. coli so they grow faster. Often people will conduct a transformation, screen the cells, and grow them up in broth for plasmid extraction. This growth would be for 12-16 hours. Instead a person could use terrific broth to grow up the cells and it would take may 6-8 hours for adequate cell density for ...
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