Tag Info

To be honest I know very few about enzymes and absolutely nothing about Michaelis–Menten. However, when I "took the Michaelis-Menten equation, replaced v with 0.4Vmax, canceled the Vmaxes (one on each side), and solved for [S]", my result is positive: 0.4 * Vmax = S * Vmax / ( Km + S ) 0.4 = S / ( Km + S ) S = 0.4 * Km + 0.4 * S 0.6 * S = 0.4 * Km S = Km * ...

Have you heard of something known as "Occam's Razor" ? It says when you have multiple possible explanations/hypotheses then select the one which is simplest (i.e least number of assumptions) Same with mathematical models. Chemical kinetics models usually assume first order unless there is some evidence against it. Similarly, for enzyme kinetics, as long ...

This expands my comment on the question to an answer. If an enzyme exhibits Michaelis-Menten kinetics, then it is valid to define a KM and this equates to the substrate concentration when reaction velocity is 0.5 * Vmax. However, many enzymes do not exhibit Michaelis-Menten kinetics. One example is when the enzyme shows a co-operative response to ...

As Arthur Kornberg said: "Don't waste clean thinking on dirty enzymes." Discovering an assay for a biological event in a cell-free extract opens the way to its molecular resolution and reconstitution (Commandment I). Trying to devise a mechanism with a crude extract, even with ingenious experiments, is generally a waste of effort. An extract is too ...

The answer is no really, but some variants might allow you to study inhibition. Michaelis Menten kinetics are experiments which try to characterize the catalysis characteristics of a reaction but to do so the numbers are obtained at concentrations of substrate that you dont find in a cell. Vmax is defined as the maximum rate at which the reaction is run ...