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Yes, fixation of almost any kind can have effects on morphology. When you take a free flowing, protein spiked fat-blob (ie cell membrane), and make it rigid, you are going to get some differences. A fun visualization of this can be done by wrapping cellophane/shrink wrap around a serological pipette and dipping it in a dry ice and methanol bath. It's ...


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Sample preparation for protein gels is not a complex task. Simply mix the appropriate amount of sample buffer with your sample and load it. For a 2x sample buffer use equal amounts of sample and buffer, for 5x sample buffer use 4 parts of sample and 1 part of buffer (for examle 40µl + 10µl). Heat the mixed samples for 5 minutes at 95°C, cool them immediately ...


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                                                    Image is of an 18% Tris-tricine small-peptide ...



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