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3

I have never had problem with tubes leaking. However, if you are worried about them getting somehow popped open in transit, you might consider wrapping the lid in parafilm. Alternately, there are lid locks that you could use like these. @Chris has a good point about screw-cap tubes, they could also work, and are probably a lot sturdier, in case of actual ...


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You have to remove the salts, remove the dye, digest the protein, extract the digested peptides from the gel etc. For details, see this protocol.


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2-mercaptoethanol (2ME) reduces the disulphide bonds in proteins. If disulphide bonds are connecting two polypeptide chains ("intermolecular") then 2ME would cause them to separate and therefore instead of a higher molecular weight (MW) band you would get one or more lower MW bands. However, if there are disulphide bonds in the same polypeptide chain, they ...


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OD600 is usually used in molecular biology as a measurement of the density of bacteria in a sample. That frequency of light typically has little other scattering and the bacterial bodies The interpretation of a specific absorbance is by Beer's law: A = abc a = specific absorbance of the light at that frequency by the specimin b = the concentration of ...


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I have had this problem before... this is what I ended up using: https://www.amazon.com/Invisible-Blacklight-Marker-Blue-Yellow/dp/B004C89M9Q You may have to write on a tape or something so that the ink doesn't come off. (Ink may come off if you incubate plates)


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Short Answer - If the same antibody works both for IHC and SDS-PAGE it either means it is monoclonal against a surface linear epitope or a polyclonal antibody. Long Answer - Starting off, there are broadly two kinds of antibodies based on the type of the epitopes. The epitope can either be linear or conformational. Here's a couple of pictures that should ...


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Many but not all B cell epitopes will be destroyed by denaturing. Those that are conformational will be destroyed, but there are also many B cell epitopes that are linear, based on amino acid sequence only without strong conformation dependence. Also, depending on your IHC protocol, there's often a fixation step that involves a certain amount of ...


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Neither of the current answers fully answer the question. The amount of liquid underneath a coverslip should be just sufficient to hold the coverslip and sample onto the slide. If there is too much water, the surface tension would become reduced, allowing the coverslip to fall off the slide. Additionally, minimising the amount of water between the ...


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Well, from personal experience, whenever there was excess solution on the glass slide, our teacher always commented that our slides were messy. So I guess the entire idea is to keep the slide neat, clean and to make it look presentable for observation. Also, in case the excess solution flows out to the entire surface of the glass slide, the stage clips may ...


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If I understood the question, it's just because otherwise the solution would flow out and stain the microscope.


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I suggest you to try Recursive Directional Ligation. It is meant to do exactly what you are aiming for, i.e. to "polymerize" short DNAs into a longer one. You can find the protocol here http://pubs.acs.org/doi/abs/10.1021/bm015630n


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I have been working with BCI since some time and would recommend you to try these ones. They all have been widely used in MS/PhD research and their results are more or less accepted everywhere: Emotiv NeuroSky (Starts from 99$) g.tec (Most accurate/Expensive one - not recommended for startups) P.S: Last, but not least, feel free to have a look at BCI ...


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I can't tell if you're asking about glassware or work surfaces (hoods, benches etc), but... We use regular old dawn dish soap in my lab because what's more important than the soap you use to wash you're glassware is the water you use to rinse it. We teach our undergrads the 3-rinse-rule. After soaping, every item gets 3 rinses with normal tapwater (or ...



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