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1

Well, you already mentioned it. Random mutagenesis (with UV or chemical mutagens) targets the entire genome and you select the organism for certain traits. In a directed evolution experiment, you are just targeting a gene or a group of genes. With the progress of synthetic biology, it might be possible to synthesize small genomes (already shown by Craig ...


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Since these are fluorescent dyes, the most likely method of use is with some sort of fluorescent (likely confocal) microscope. The various types of dyes have different hydrophobic tails, so they target different types of membranes (plasma membrane, mitochondrial membranes, etc.). An excitatory light source (typically a laser, with a single wavelength) is ...


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As far as I know you can either adjust the ampere or the voltage as both is dependent of the resistance from the buffer. Which means that I would suggest you to let the voltage as it is. As the ampere = voltage / resistance and voltage = resistance * ampere and as you can see that the voltage has to get higher if the ampere is increased and the ampere has ...



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