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2

If they are available, talk to the people who analysed your data. Using their knowledge is intelligent. I will assume two critical steps are completed. First that your results have been searched correctly. I will also assume that the peptide and protein identifications have been filtered to provide a false discovery rate of 1:100 at each level. You can ...


1

For most laboratory spectrophotometers the path length in cuvette that holds your sample is 1 cm, and unless another path length for the light is specified you can always assume that the Beer-Lambert Law for absorption of light is using 1 cm. When growing a bacterial liquid culture in an incubator, the OD (wavelength is usually 550 nm or 600 nm) is a ...


9

1x is the final working concentration of the solution (it could be anything depending on the type of the solution). Stock solutions are made at a higher concentration; if it is 10 times more concentrated than the working solution then it is 10x. Why 50x TAE but 10x TBE? The salts in TBE precipitate at higher concentrations. In other words, the salts ...


4

I used to work for a company well-known for its modification state-specific antibodies, including phospho-specific ones, and they actually performed extensive in-house testing of PBST vs. TBST in Western blotting. Part of the reason the company chose to recommend the use of Tris-buffered saline over phosphate-buffered saline based buffers was the clearly-...


0

Formaldehyde forms adducts with RNA and can also make it more susceptible to degradation. It can also cause crosslinks to form between RNA and proteins (or possibly other RNAs). For all these reasons extraction of high quality RNA from formaldehyde fixed samples is very difficult and the yields are low (Howat and Wilson, 2014). Have a look at this article ...


1

You could be measuring outside the dynamic range of the assay (you can only add so much / so little BSA to a certain amount of reagent). You could be outside the linear range of your spectrophotometer, usually under 1 is fine, although some old/crappy ones might not even be reliable over 0.5. If you're fast you're measuring your different concentrations ...



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