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RNAeasy kit will not usually give phenolic contamination. Your poor 260/230 is perhaps because of low yield; check the absolute absorbance values. Low 260/230 (and low yield) also happens because of improper lysis. I would advise that you do the cell lysis using more effective methods. I would not advise another phenol-chloroform or alcohol precipitation ...


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Just finished an experiment (yesterday) where I measured lifetime reproductive success (LRS) of a few hundred flies, with measures taken from 24 hours of production every 7 days over the entire life of the fly. From this I can now answer the question. Data will hopefully be published (as part of a larger project) in the near future. Correlations of time ...



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