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In addition to biochemical methods, a lot of this was figured out using radioactive tracers. Cells would be fed a radioactive substrate and as they convert the substrate to other molecules through metabolic reactions, the radioactivity can be detected in the products and intermediates. Calvin's 1940s-50s experiments to establish carbon flow during ...


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The short answer is no, you do not study metabolism under the microscope. (Modern high resolution microscopes can visualize macromolecular processes like transcription and translation, but that is not how they were studied originally.) In brief, biochemical pathways were studied in broken cells by studying individual reactions or in whole tissues or ...


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There are optimal temperature, pressure and substrate concentration for each biochemical process. The optimal temperature and pressure for two or more (but not all) processes may overlap. So there is no SINGLE temperature or pressur where ALL of them will be at their maximum. Please note, by mentioning "cellular processes" I meant all the biochemical(e.g ...


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Coupling process by which two or more chemical reactions depend on each other through energy once one is exothermic another is endothermic, one produce product or intermediate which is used by the another Examples glycolysis and citric acid cycle , phosphorylation and dephosphorylation in steps of glycolysis and many other


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The answer is in special situations, where a trade-off has to be done between thermogenesis and energy saving. This applies to hibernation of animals as well as to critical illness in humans. This kind of adaptation is referred to as TACITUS syndrome (thyroid allostasis in critical illness, tumours, uraemia and starvation), NTIS (non-thyroidal illness ...


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According to David H. Vroon and Zafar Israili aminotransferases (transaminases) are widely distributed among tissues, and are found in both cytoplasm and mitochondria, although this may vary between different aminotransferases: Aminotransferases catalyze the redistribution of nitrogen between amino acids and corresponding oxoacids participating in both ...


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The oxidative deamination takes place inside the mitochondria, because the glutamate dehydrogenase is localized in the mitochondria. About the transamination, I'm not sure if it occur inside the mitochondria or in the cytoplasm. The information about the oxidative damination and the enzyme could be found at this link: http://www.bioinfo.org.cn/book/...


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These enzymes, while useful markers of disease, are not only produced during the disease state, and are not only produced in one organ. It is therefore not surprising that there are always low levels released into the biochemical milieu that is the blood. If there are not even low levels of the enzymes then that might indicate that the organ is not doing it'...



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