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This question got me thinking about what are the metabolic enzymes that take oxygen up in E coli. I searched the metacyc database for reactions that consume molecular oxygen and there are only 3 that take in oxygen and one that produces oxygen. All three consumers of oxygen in E coli are the oxidation of ubiquinone by at two sites in cytochrome bcl or by ...


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The plasma membrane is quite permeable to oxygen and thus oxygen enters the cell simply by diffusion. Reactive oxygen species can be reduced enzymatically in aerobic organisms. Obligate anaerobes lack or don't produce sufficient quantities of these enzymes. An organism that doesn't use oxygen for metabolism but is also not relatively harmed by it can be ...


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If you are speaking especially of the stomach, it actually makes no difference because the extremely low pH of the stomach allows very few bacterial organisms to survive. Often, Helicobacter pylori can however be present. Whether dissolved oxygen makes a difference to it is, to my knowledge, unknown. However, pure water does not stay long inside the stomach ...


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Decay is a process where the body is digested by bacteria, fungi and other living things. The moon has no known biological processes and while its possible for some microorganisms to survive in space, I think its generally understood that the lunar surface would not support life in a significant way, especially if the body is in vacuum. Other processes ...


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I actually doubt that the pectinase has such a broad pH range in which it works optimally. Searching the web I found two figures which support my doubts: The first is from an article ("Immobilization of pectinase by adsorption on an alginate-coated chitin support") which compares the activity of native and immobilized pectinase under different ...


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Answer clarified based on comment by @DurgaDatta What would it mean to say the enzyme activity was tested in vitro on the mutant? When we say the response of the a single gene knock out mutant, the cell should be intact except for lacing one gene, which would mean that the response is in vivo. The knockouts were performed inside the E. coli cells and, ...


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It is possible to conduct an in vitro experiment with cell extracts. Meaning that you break up the cells and put their pieces into a reaction glass and do your experiment. It is assumed that the cell molecules (enzyme, sugar or cofactors) will still carry out their functions even though the cell is no more. In other words, you used the mutant to produce the ...


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I am assuming in spacesuit here, on the face (lit side) of the moon. Bodily degradation involves much more than external fungi and bacteria. Cells that receive no oxygen or nutrients die. We talk of such tissue death as dry gangrene when it affects extremities, such as fingers, feet, etc. However, we also recognize gangrenous bowel, etc. which results in ...



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