Hot answers tagged microbiology
Okay, I found the last part of your question the most interesting and can't give a full answer to that, but I just noticed I can answer the rest so here goes. How it works (the simple version) It works on the principles of buoyancy. The eggs and oocysts are less dense (lighter) than the fecal flotation solution and, consequently, they float on the very ...
Why Bilayer and not a Monolayer Lipid monolayer vesicles are possible as you mentioned (for example micelles). However, you have to understand that the cellular interior i.e. the cytoplasm, is aqueous and therefore a monolayer vesicle like micelles would not work. In micelles, the two compartments - interior and exterior, have to be of opposite nature for ...
Apparently not for lactase and β-galactosidase, as one might expect for this sort of hydrolytic reaction. There are also Wikipedia entries for maltase, sucrase and trehalase with references you can follow up to check yourself.
It seems that beta-glucosidases do not require co-factors but some alpha-glucosidases are co-factor dependent. Some bacterial alpha-glucosidases (GH4 family) require NAD+ to function (Hall et al., 2009). Thermotoga maritama alpha-glucosidase (GH4 family) requires Mn2+, in addition to NAD+ to function (Lodge et al., 2003). Ferroplasma acidophilum alpha-...
I have had this problem before... this is what I ended up using: https://www.amazon.com/Invisible-Blacklight-Marker-Blue-Yellow/dp/B004C89M9Q You may have to write on a tape or something so that the ink doesn't come off. (Ink may come off if you incubate plates)
The optical density measurement is the quantity of living and non-living cells in a sample. The colony forming unit measures only the viable cells in a sample. An OD 600nm is equivalent to approximately 8 x 10^8 CFU/ml. However, this is only an approximation and cannot fully take into account the dead cells the OD may be reading, it also depends on the ...
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