New answers tagged microbiology
Plasmids do have the advantage that they replicate independently of the cells chromosome and that they can be transferred between different bacteria. This spreads the gene encoded by the plasmid to other bacteria, which then can also survive. Think about antibiotic resistance. Bacteria which have a plasmid which allows protection against the antibiotic will ...
Ventricaria ventricosa (previously called Valonia ventricosa) is not exactly a single cell. It has a coenocytic structure with multiple nuclei and chloroplasts. As Jasand Pruski correctly guessed the organism possesses a large central vacuole which is multilobular in structure (lobules radiating from a central spheroid region). The entire cell contains ...
The T7 promoter is encoded for by the genome of T7 bacteriophage. The T7 RNA polymerase is specific for the T7 promoter, and so in the presence of the T7 promoter the T7 RNA Polymerase will transcribe everything going 5'-3'. If your T7 promoters are flanking your gene of interest, you can expect copies of your coding portions either way the insert goes in. ...
You will definitely get satellite colonies. Ampicillin breaks down after about a day, especially exposed to light. Kan, keeps pretty well at room temp, but that does you no good :)
You can see this protocol for Wolbachia isolation: See this protocol for infecting cell lines with Wolbachia. In this study the authors have established a cell line from a Wolbachia infected tissue of Aedes albopictus. You can have a look at that too.
While I doubt that this might be possible in all cases (and I would be careful about classifications), there are indeed some great ressources, which contain a lot of images. Since there is a vast number of bacteria present on this planet, there is of course a limitation to bacteria available in photos, representing only the most common or important. These ...
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