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11

First off I'd like to reccomend the University of Dartmouth's publicly available collection located here. They have both SEM and TEM images of a wide range of organisms and cells from algae to see urchins through everything from cholera to mammalian cells. Images are high quality, fully captioned and properly attributed. I'm a little confused as to the ...


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David Orloff's The Cell – an Image Library has thousands of SEM images.


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Have a look at: XTALENT Image Gallery, more than 600 images, last updated in 2006, though. Dennis Kunkel Microscopy, Inc., "scientific stock photography library of light microscope pictures and electron microscopy images featuring science and biomedical microscopy photos". Iowa State University SEM library, includes pictures submitted by students from ...


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The word to look for is 'Image segmentation'. But also here, it very much depends on what you're looking at. Segmentation and quantification of simple fluorescence images is relatively easy and widely used. It can get very complex depending on how complex the structure is you're looking at. There are machine-learning approaches which are able to distinguish ...


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Taken right from the Wiki page you linked to: AFM only images the surface of a specimen, to a maximum depth of 10-20 µm and a maximum scan area of 150 µm x 150 µm. Compared with scanning electron microscopy, SEM has a much larger depth of penetration and scanning area (~1 order of magnitude greater). AFM is also a much slower scanning ...


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To start with, CLARITY is a whole-brain imaging technique , there is no need for sectioning which destroys connections and can cause the brain slices to warp as they are being cut. The costs for setting up CLARITY are much lower because all that is needed is chemicals to get the prep ready for imaging whereas in the Nat Methods paper you have to set up an ...


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The website you want is The Cell: An Image Library. They also have movies. A quick search of 'Homo sapiens tumor' revealed several Creative Commons videos of tumor cells moving around in vitro with attribution and no annotation or anything else. Sometimes the site can be a bit buggy, but it's a pretty awesome resource when it works.


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I think you have a good question, but if you want to get a good understanding of the issues you raise with it, then you really ought to consider spending some time reading this optical microscope primer. In my opinion, you need not bother with taking an undergraduate course at a physical university. As an intro to biology (which is not really necessary if ...


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When testing an antibody for an imaging application, it is almost always a good idea to test it in another application like ELISA or Western blotting to see if it binds the target of interest. For example, try to find high- and low-expressing cell lines, load equal amounts, and see if there is a difference in signal at the expected molecular weight. Check ...


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Nature Methods seems to publish quite a few methods that facilitate this kind of analysis. E.g. http://www.nature.com/nmeth/journal/v8/n3/full/nmeth.1558.html And an editorial from them: http://www.nature.com/nmeth/journal/v9/n7/full/nmeth.2102.html As part of a special issue on BioImage Informatics with some nice examples. ...


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There have been experiments with Live-cell dSTORM with SNAP-tag fusion proteins in Markus Sauer's lab in Würzburg. If you are using the physiology definition of in-vivo, I'm pretty sure some of my co-workers have measured on live organisms. However, this is work in progress and not fully published; if you are interested, you should ask Markus Sauer directly. ...


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Any superresolution techniques relying on stochastic sampling (e.g. PALM, STORM) is extremely difficult to accomplish in vivo, as the tissue would be moving (e.g. due to respiration) and this would hinder a lot the reconstruction of the image. Apparently it may be possible to do it with STED but I could not find the related paper*, although the same authors ...


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I agree with Kevin F, if I was you, I'd take a bit of training before buying something or even trying. Biology teachers or staff from the university are usually happy to provide you with a microscope to test something, so just ask. If you want a resolution that high, there are a lot of adjustments to do, commonly referred to as "Köhler illumination", because ...


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Despite low resolution, I'd say those are trichomes. Jasminum is genera that produce essential oils, so it have glandular hairs and other trichomes wich help to rettain the oil layer. Source: http://cms.herbalgram.org/herbalgram/issue53/article2207.html?ts=1376643526&signature=b4349f7f3ca6f6a47823f4754237eaac The image displayed above shows glandular ...


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Most of the dyes used for visualization bind with a much higher affinity to dsDNA. This would be SybrGreen, EtBr (although this will bind RNA as well). There is a pretty comprehensive website from Life Technologies about Nucleic acid stains that is worth a look. There is as well a publication on this topic: "DNA Staining for Fluorescence and Laser Confocal ...


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Typically wet mounts are used to observe the mobility and behavior of microorganisms. A classical use for a wet mount would be to study something like pond water were you could see all the different organisms swimming around like they would in their natural environment. Oils are more viscus and would impede mobility and might impact behavior or even be toxic ...


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Well you can always use a piece of white circular paper and draw the outline of the light onto it since it should be visible. This is a quick and dirty method that can be improved by taking a photo of the light with a camera if you have one and then comparing the distance of the edge of the paper measured with a ruler to that of the circle of light.


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I found this paper. Zhang GJ et al. (2009) In vivo optical imaging of LacZ expression using lacZ transgenic mice. Assay Drug Dev Technol.7:391-9. doi: 10.1089/adt.2009.0195. Abstract beta-Galactosidase (beta-gal) (encoded by the lacZ gene) has been widely used as a transgene reporter enzyme. The ability to image lacZ expression in living ...


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Higher magnification is definitely NOT always better. Indeed, the microscope you are considering is designed for maximum magnification of 1,000x. The 2,000x is achieved by adding 20x eyepieces BUT it creates what is known as 'false magnification'. 2,000x is beyond the resolving power of a light microscope so what happens is that the image is magnified 2,000x ...


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This is sort of technical answer, but I hope its helpful I hope. All these bands in the sarcomere are anisotropic. Isotropy would imply that the structure/properties of something is equally ordered or coherent in all 3 directions. The sarcomere has definite structure along its longer axis so the H band is anisotropic. Since the H band is a small ...


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You will get a lot of false-positives using the following method, and a real transfection of a fluorescent protein is always the way to go, because then you will prove that the transfection was really successful. That said, you could try to use DAPI, followed by fluorescence microscopy or flow cytometry. DAPI is a very bright stain for DNA and cannot pass ...


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The lateral and axial resolution depends on the numerical aperture of your objective (lateral = lambda/2NA; axial = 2lambda/NA^2). You can improvee the axial resolution with FLI by using a confocal approach or with 2-photon microscopy (2PM). In 2PM you need absorption of two infrared photons to get emission. The probability of 2 infrared absorption depends ...



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