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First off I'd like to reccomend the University of Dartmouth's publicly available collection located here. They have both SEM and TEM images of a wide range of organisms and cells from algae to see urchins through everything from cholera to mammalian cells. Images are high quality, fully captioned and properly attributed. I'm a little confused as to the ...

David Orloff's The Cell – an Image Library has thousands of SEM images.

Have a look at: XTALENT Image Gallery, more than 600 images, last updated in 2006, though. Dennis Kunkel Microscopy, Inc., "scientific stock photography library of light microscope pictures and electron microscopy images featuring science and biomedical microscopy photos". Iowa State University SEM library, includes pictures submitted by students from ...

The word to look for is 'Image segmentation'. But also here, it very much depends on what you're looking at. Segmentation and quantification of simple fluorescence images is relatively easy and widely used. It can get very complex depending on how complex the structure is you're looking at. There are machine-learning approaches which are able to distinguish ...

Taken right from the Wiki page you linked to: AFM only images the surface of a specimen, to a maximum depth of 10-20 µm and a maximum scan area of 150 µm x 150 µm. Compared with scanning electron microscopy, SEM has a much larger depth of penetration and scanning area (~1 order of magnitude greater). AFM is also a much slower scanning ...

I think you have a good question, but if you want to get a good understanding of the issues you raise with it, then you really ought to consider spending some time reading this optical microscope primer. In my opinion, you need not bother with taking an undergraduate course at a physical university. As an intro to biology (which is not really necessary if ...

When testing an antibody for an imaging application, it is almost always a good idea to test it in another application like ELISA or Western blotting to see if it binds the target of interest. For example, try to find high- and low-expressing cell lines, load equal amounts, and see if there is a difference in signal at the expected molecular weight. Check ...

Nature Methods seems to publish quite a few methods that facilitate this kind of analysis. E.g. http://www.nature.com/nmeth/journal/v8/n3/full/nmeth.1558.html And an editorial from them: http://www.nature.com/nmeth/journal/v9/n7/full/nmeth.2102.html As part of a special issue on BioImage Informatics with some nice examples. ...

There have been experiments with Live-cell dSTORM with SNAP-tag fusion proteins in Markus Sauer's lab in Würzburg. If you are using the physiology definition of in-vivo, I'm pretty sure some of my co-workers have measured on live organisms. However, this is work in progress and not fully published; if you are interested, you should ask Markus Sauer directly. ...

Any superresolution techniques relying on stochastic sampling (e.g. PALM, STORM) is extremely difficult to accomplish in vivo, as the tissue would be moving (e.g. due to respiration) and this would hinder a lot the reconstruction of the image. Apparently it may be possible to do it with STED but I could not find the related paper*, although the same authors ...

I agree with Kevin F, if I was you, I'd take a bit of training before buying something or even trying. Biology teachers or staff from the university are usually happy to provide you with a microscope to test something, so just ask. If you want a resolution that high, there are a lot of adjustments to do, commonly referred to as "Köhler illumination", because ...

You will get a lot of false-positives using the following method, and a real transfection of a fluorescent protein is always the way to go, because then you will prove that the transfection was really successful. That said, you could try to use DAPI, followed by fluorescence microscopy or flow cytometry. DAPI is a very bright stain for DNA and cannot pass ...