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ImageJ is a multi platform piece of software that has a cell counter module that might be of some use, and hey its free! Its easy to use and so ludicrously crude that it is very versatile. I remember using it in undergrad to count cells under the microscope automatically after a few image contrast tweaks. I don't see why this couldn't be reapplied to count ...


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The book "Basic Histology" by Junqueira and Carneiro acknowledge the problem in histologic fixation of ground substance. They recommend freeze drying in liquid nitrogen and then removing water by high vacuum at a temperature of -30 celcius, which will cause sublimation (ice-->vapor). Then fixing the sample with a nonpolar solvent. Afterwards you can use ...


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Different components of the ECM can be stained differently but since you asked about hyaluronic acid (HA) I'll limit my answer to its staining. See this paper. They use Hyaluran Binding Protein (HABP) as a specific probe for HA. Because HA has a very simple, conserved composition and is ubiquitously expressed in all animals that have a developed ...



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