New answers tagged microscopy
You may also want to look at acridine orange. It binds both single stranded and double stranded DNA, but fluoresces green when bound to double sided DNA, and orange when bound to single stranded DNA/RNA, making it very easy to see both in the same image.
There are two common ways to generate a scale bar: Measure it. You can take a picture of an object with known spacing such as a grid target and then measure the size of each pixel. Compute it. If you know the magnification of your objective, and the magnification of any relay optics after the object but before your camera, you can compute the total ...
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