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Some things to consider. If you spinning too fast and too long, ti's going to pack the pellet more. You can spin longer at a slower speed and you will notice your pellet is not as packed. I always do 2000 rpm, 20 minutes, 4C if I'm doing a midi or maxi sized prep and harvesting bacteria in 50ml conical's. If I'm doing a miniprep I will take the 1.5 micro ...

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