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For assaying effect of alcohol on cell growth:     Prokaryotic Cells Take ~5ml medium (LB for E.coli) in test-tubes/plastic tubes and add appropriate concentration of alcohol(s) in these. Inoculate 1% bacteria from a starter culture (OD~0.6) After different time intervals or a fixed time point take some culture, dilute and spread ...


4

Based on T Abraham's answer, a hemocytometer would work. However, a hemocytometer requires a microscope, but if you are in a cell lab, you probably have access to a microscope. I would recommend using mammalian cells instead of bacteria, they're larger and easier to see, and more relevant to human health. If you're interested in liver toxicity in particular, ...


4

Great idea! It seems that this research has been done before and your hypothesis is correct, but testing a wide variety of alcohols and testing your hypothesis for each one of them would be a great project. Now, to do this, I would expect you would need to check the growth of the cells after certain periods of time. How would you do this? Well, the best ...


2

This specific experiment has not been done. Fusing cells is difficult and it also leads to polyploidy. Cells are immortalized by overexpressing oncogenes (or viral replication genes); for example HEK293 cell line was established by transforming embryonic kidney cells with adenovirus. Now transforming a neuron would immortalize it but would also make it ...



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