New answers tagged molecular-genetics
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First we must remember what a nucleosome is: a segment of DNA packed with proteins called histones. This is the initial step to turn the DNA a more compact structure. Citing the advantages of an extremely compact but flexible structure like chromosomes would make this answer a little longer (if you want, I can add them later), and to answer your question ...
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This is controversial, but most groups in the field have concluded that RNAi does not work in the nucleus despite the presence of Ago. When lncRNA have been KD'd with RNAi, they are usually shown to be present in both the nucleus and cytoplasm, and the KD is thought to occur in the cytoplasm, not nucleus. Of course as always in biology it is possible that ...
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Knockdown of lncRNA in mammals is not done via RNAi. Instead, one transfers antisense DNA oligos which bind to the RNA. This triggers the action of the RNase H enzyme, which degrades RNA-DNA duplexes. It degrades the lncRNA.
UPDATE: For reference, I learned about this from a seminar, and it is not very well documented, but after some literature search I ...
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Nuclear RNAi happens.. check these articles:
http://www.nature.com/nrg/journal/v14/n2/execsumm/nrg3355.html
http://nar.oxfordjournals.org/content/early/2011/11/07/nar.gkr891.full
Also there is some evidence that Ago2 binds to lncRNAs.
However, you can employ other techniques to knock down lncRNAs for e.g. ribozymes.
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This stage is after the adsorption of the adapter-ligated fragments on the flow cells.
The fragments are isothermally amplified to generate clusters [1]. The exact nature of the enzyme mix that catalyses this step is not disclosed by Illumina. I assume it must be similar to Helicase mediated isothermal amplification, which uses helicase and single ...
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The v short answer is that in shotgun sequencing, the sequencer is fed a set of random sequences from the target. This can be done for instance by mechanically shearing DNA and then building a set of shotgun sequencing jobs which are then compiled back together by a genome assembler (i.e. in whole genome sequencing projects) into a full sequence.
In ...
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