New answers tagged mutations
A retransformation should get you a pure plasmid. Transform the plasmid mix into empty E.coli (don't use too much DNA), plate on Kan / Amp depending on what you need. The probability that one cell took up both plasmids is extremely low, and you could check with colony PCR just to be sure. Otherwise: ask around, there must be (old) stocks of the single ...
As 'c.' stands for 'coding' sequence (instead of 'g.' for 'genomic'), according the HGVS guidelines it denotes the A to T substitution at nucleotide -35 of an intron (in the coding DNA positioned between nucleotides 1842 and 1843).
Answers Possibly, but with such a low frequency as to be unimportant and undetectable. (Monkeys with typewriters producing Shakespeare’s Hamlet comes to mind.) No. Because it would be extremely difficult to detect, it would seem to be of no importance if it did occur at a very low frequency (you make no suggestion of why it would be of interest) and ...
So the term allele is a broad one, and simply refers to the different versions of any piece of DNA in circulation in the gene pool - it doesn't need to refer to a gene. I can talk about the alleles at a random place in the genome. But if we proceed with your question and ask - 'do nonsense mutations within coding genes also lead to the creation of different ...
Allele is just a variant form of gene: independent of the final product of protein, so nonsense will also lead to new allele. I will quote Nature Scitable here: Alleles can also refer to minor DNA sequence variations between alleles that do not necessarily influence the gene's phenotype.
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