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First of all, if this is a homework question, add that tag to it. And show what work you have done so far. Secondly, primers for amplifications should lie on opposite strands. Primers are typed in 5'-to-3' direction (aka left-to-right on leading strand). Appropriate primers will be: primer 4=GTG... and primer 5=GAA.... Note how those primers are always in ...


0

In any PCR reaction ( including the assembly PCR ) the substrate of the reaction is a mix of mono nucleotides. Specific oligos serve as primer by annealing on the template and that are extended by a DNA polymerase. It is a polymerization reaction. In the LCR there are no mono nucleotides instead you have many different oligos that anneal one after the other ...


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In the paper by K. Lily Therese, U. Jayanthi & H.N. Madhavan traditional PCR wasn't sensitive enough to allow for an accurate reading on what they were trying to study. By using nPCR they were able to amplify around the gene they were interested in and then amplify just the gene they were interested in for a more accurate and sensitive study. The ...



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