New answers tagged pcr
If it was detectable in a microarray, the odds are very good for RT-rtPCR. If you are designing your own primers, make sure they span an exon junction, or if the gene is intronless, then span the UTR to exon junction to avoid amplifying DNA.
There are two different ways to detect PCR product in real-time PCR: dyes which bind to DNA in general, such as SYBR Green I, or probes specific to your PCR product. There's a summary of the benefits of each here, with the following table: You can buy either online; they'll be fairly expensive but I can't think of an obvious way to get them cheaper if you'...
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