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2

PCR efficiency is not usually expressed as percentage (or per-unit). Its bounds are [0 2] i.e the maximum efficiency is 2 and the minimum is 0. (Ruijter et al. 2009; also see this post for calculation of efficiency.) However, I just noticed that some people do express efficiency as percentage or per-unit. In that case your formula would be correct. I ...


3

The Taq polymerase is a rather fast enzyme (the synthesis rate is around 1 kb/min), but the backdraw is that it has no 3'- 5'exonuclease activity which would enable to proofread the newly synthesized DNA. This leads to the introduction of errors and the chance of getting these is higher the longer the DNA you want to synthesize is. Polymerases which are ...



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