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When we say that something is a genetic marker we mean that we can establish its linkage to a chromosome AND that we have some way of discerning, or detecting, how the marker has segregated after meiotic recombination (this definition is only valid for diploid species that undergo sexual reproduction). So, for example, in the fruit fly, Drosphila ...


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Short answer is that higher temperature favors annealing of longer sequences. There are number of ways to calculate melting temperature, but all of them produce similar results: longer polymers require more thermal energy to melt. Hence, quick cooling from higher (say, from 95C thermocycler can cool in 10-12 sec) to RT/4C will favor re-annealing of circular ...


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I think you can follow this method: Test the statistical significance of a single experiment by comparing technical replicates (lets say Tests: T1, T2, T3 and Controls: C1, C2, C3). This will tell you if the instrument can reliably detect difference or not. Note that you should compare the relative expression (i.e. wrt your reference gene) and not the CT ...



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